PLGA nanoparticles optimized by Box-Behnken for efficient encapsulation of therapeutic Cymbopogon citratus essential oil

PLGA nanoparticles optimized by Box-Behnken for efficient encapsulation of therapeutic Cymbopogon citratus essential oil

[Display omitted] •Box-Behnken design allows establishing the optimal experimental conditions.•Only citral was incorporated into the optimized PLGA nanoparticle.•Citral release is dependent on its diffusion from polymeric matrix.•Nanocarrier improves physicochemical characteristics of lemongrass ess...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces Vol. 181; pp. 935 - 942
Main Authors: Almeida, Kessiane B., Ramos, Aline S., Nunes, Júlia B.B., Silva, Bianca O., Ferraz, Elisa R.A., Fernandes, Andreia S., Felzenszwalb, Israel, Amaral, Ana Claudia F., Roullin, V. Gaëlle, Falcão, Deborah Q.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-09-2019
Subjects:
Box-Behnken design
Cell Line
Cell Membrane - drug effects
Cell Survival - drug effects
Cymbopogon - chemistry
Cymbopogon citratus
Cytotoxicity
Drug Carriers - chemistry
Drug Liberation
Essential oil
Humans
Kinetics
Nanoparticles - chemistry
Nanotechnology
Oils, Volatile - chemistry
Oils, Volatile - pharmacology
Particle Size
Polyglycolic Acid - chemistry
Polymeric nanoparticles
Surface Properties
Cytotoxicity
Essential oil
Polymeric nanoparticles
Cymbopogon citratus
Box-Behnken design
Nanotechnology
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Summary:[Display omitted] •Box-Behnken design allows establishing the optimal experimental conditions.•Only citral was incorporated into the optimized PLGA nanoparticle.•Citral release is dependent on its diffusion from polymeric matrix.•Nanocarrier improves physicochemical characteristics of lemongrass essential oil.•Cytotoxicity of pure essential oil was significantly reduced by nanoencapsulation. This study aimed to optimize Cymbopogon citratus essential oil loaded into PLGA-nanoparticles by investigating the effect of processing variables (sonication time, ultrasound power, and essential oil/polymer ratio) on encapsulation efficiency and particle mean hydrodynamic diameter using Box-Behnken design. Nanoparticles were prepared by an emulsification/solvent diffusion method and physicochemically characterized by FTIR, DSC and TGA/DTA. Cytotoxicity was evaluated in human HaCat keratinocytes by WST-1 and LDH assays. The optimized formulation had a hydrodynamic mean diameter of 277 nm, a polydispersity index of 0.18, a Zeta potential of −16 mV and an encapsulation efficiency of 73%. Nanoparticle characterization showed that only citral was incorporated in nanocarriers, with some amount adsorbed on their surface, and highlighted the potential in increasing the oil thermal stability. The drug release profile demonstrated a biphasic pattern with a substantial sustained release depending on diffusion from the polymeric matrix. Toxicity effects on cell viability of pure essential oil at low concentrations were significantly eliminated when encapsulated. Results revealed the ability of PLGA-nanoparticles to improve essential oil physicochemical characteristics, by controlling release and reducing toxicity, suggesting their potential use in pharmaceutical preparations.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2019.06.010