Evaluation of Saccharomyces cerevisiae modified via CRISPR/Cas9 as a cellulosic platform microorganism in simultaneously saccharification and fermentation processes

Evaluation of Saccharomyces cerevisiae modified via CRISPR/Cas9 as a cellulosic platform microorganism in simultaneously saccharification and fermentation processes

The nonrenewable character and deleterious effects of fossil fuels foster the need for cleaner and more inexhaustible energy sources, such as bioethanol. Especially from lignocellulosic biomasses. However, the economic viability of this product in the market depends on process optimization and cost...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering Vol. 46; no. 8; pp. 1111 - 1119
Main Authors: de Mélo, Allan H. F., Nunes, Alexia L., Carvalho, Priscila H., da Silva, Marcos F., Teixeira, Gleidson S., Goldbeck, Rosana
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-08-2023
Springer Nature B.V
Subjects:
Bagasse
Biofuels
Biotechnology
Cellulose - metabolism
Chemistry
Chemistry and Materials Science
CRISPR
CRISPR-Cas Systems
Energy sources
Environmental Engineering/Biotechnology
Ethanol
Ethanol - metabolism
Fermentation
Food Science
Fossil fuels
Fungi
Gene expression
Glucosidase
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Inoculum
Lignocellulose
Mathematical models
Optimization
Research Paper
Saccharification
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharum - metabolism
Sugarcane
Yeast
β-Glucosidase
Bagasse sugarcane
Bioprocesses
CRISPR/Cas9
SSF
Design experiment
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Summary:The nonrenewable character and deleterious effects of fossil fuels foster the need for cleaner and more inexhaustible energy sources, such as bioethanol. Especially from lignocellulosic biomasses. However, the economic viability of this product in the market depends on process optimization and cost reduction. This research applied a sequential experimental project to investigate the process of enzymatic saccharification and simultaneous fermentation to produce ethanol with sugarcane bagasse. The differential of the work was the application of the strain of Saccharomyces cerevisiae AGY001 which was improved by evolutionary engineering to become thermotolerant and by a heterologous expression based on genomic integration by CRISPR/Cas9 to produce endoglucanase and β-glucosidase (AsENDO-AsBGL). The maximum ethanol yield found was 89% of the maximum theoretical yield (released sugars), obtained at temperature concentrations, sugarcane bagasse and inoculum at 40 °C, 16.5%, and 4.0 g/L, respectively (12.5 FPU/g bagasse). The mathematical model obtained can predict approximately 83% of the data set with 95% confidence. Therefore, these findings demonstrated the potential of sugarcane bagasse and S. cerevisiae AGY001 strain (CRISPR/Cas9 modified) in bioethanol production without the need for impractical selection media on an industrial scale, in addition to providing useful insights for the development of SSF processes.
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ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-022-02765-1