Affibody-Indocyanine Green Based Contrast Agent for Photoacoustic and Fluorescence Molecular Imaging of B7–H3 Expression in Breast Cancer

Affibody-Indocyanine Green Based Contrast Agent for Photoacoustic and Fluorescence Molecular Imaging of B7–H3 Expression in Breast Cancer

Spectroscopic photoacoustic (sPA) molecular imaging has high potential for identification of exogenous contrast agents targeted to specific markers. Antibody–dye conjugates have recently been used extensively for preclinical sPA and other optical imaging modalities for highly specific molecular imag...

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Bibliographic Details
Published in:Bioconjugate chemistry Vol. 30; no. 6; pp. 1677 - 1689
Main Authors: Bam, Rakesh, Laffey, Makenna, Nottberg, Katharine, Lown, Patrick S, Hackel, Benjamin J, Wilson, Katheryne E
Format: Journal Article
Language:English
Published: United States American Chemical Society 19-06-2019
Subjects:
Animals
Antibodies
B7 antigen
B7 Antigens - analysis
Breast cancer
Breast Neoplasms - diagnostic imaging
Contrast agents
Contrast Media - chemistry
Dyes
Female
Fluorescence
Fluorescent Dyes - chemistry
Glands
Imaging
Immunoconjugates - chemistry
Indocyanine Green - chemistry
Invasiveness
Kidney stones
Mice
Optical Imaging - methods
Photoacoustic Techniques - methods
Scaffolds
Tumors
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Description
Summary:Spectroscopic photoacoustic (sPA) molecular imaging has high potential for identification of exogenous contrast agents targeted to specific markers. Antibody–dye conjugates have recently been used extensively for preclinical sPA and other optical imaging modalities for highly specific molecular imaging of breast cancer. However, antibody-based agents suffer from long circulation times that limit image specificity. Here, the efficacy of a small protein scaffold, the affibody (ABY), conjugated to indocyanine green (ICG), a near-infrared fluorescence dye, as a targeted molecular imaging probe is demonstrated. In particular, B7–H3 (CD276), a cellular receptor expressed in breast cancer, was imaged via sPA and fluorescence molecular imaging to differentiate invasive tumors from normal glands in mice. Administration of ICG conjugated to an ABY specific to B7–H3 (ABYB7–H3-ICG) showed significantly higher signal in mammary tumors compared to normal glands of mice. ABYB7–H3-ICG is a compelling scaffold for molecular sPA imaging for breast cancer detection.
Bibliography:Author Contributions
R.B. conducted all the experiments, analyzed the data, and wrote the manuscript. M.L. and K.N. produced the contrast agent, assisted in the experiments, and analyzed the data. P.L. and B.H. identified the protein sequence used in the contrast agent. K.E.W. designed and conceptualized the study and aided in conducting the experiments and analyzing data. All authors read and revised the manuscript.
ISSN:1043-1802
1520-4812
DOI:10.1021/acs.bioconjchem.9b00239