Functional CD40 ligand expressed by human blood dendritic cells is up- regulated by CD40 ligation

We examined whether human blood dendritic cells (DCs) express a functional ligand for CD40 (CD40L). Human blood DCs expressed significant amounts of cell surface CD40L identical to that expressed on activated T cells, as detected by mAb to CD40L or a chimeric CD40.Ig fusion protein (CD40.Ig). Stimul...

Full description

Saved in:

Bibliographic Details
Published in:	The Journal of immunology (1950) Vol. 157; no. 10; pp. 4363 - 4370
Main Authors:	Pinchuk, LM, Klaus, SJ, Magaletti, DM, Pinchuk, GV, Norsen, JP, Clark, EA
Format:	Journal Article
Language:	English
Published:	United States Am Assoc Immnol 15-11-1996
Subjects:	B-Lymphocytes - metabolism Burkitt Lymphoma CD40 Antigens - biosynthesis CD40 Antigens - metabolism CD40 Ligand Dendritic Cells - immunology Dendritic Cells - metabolism Flow Cytometry Humans Ligands Membrane Glycoproteins - biosynthesis Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Palatine Tonsil - cytology Polymerase Chain Reaction Protein Binding - immunology RNA, Messenger - analysis Tumor Cells, Cultured Up-Regulation - immunology
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We examined whether human blood dendritic cells (DCs) express a functional ligand for CD40 (CD40L). Human blood DCs expressed significant amounts of cell surface CD40L identical to that expressed on activated T cells, as detected by mAb to CD40L or a chimeric CD40.Ig fusion protein (CD40.Ig). Stimulation through CD40 up-regulated protein and mRNA CD40L expression in DCs, B cells, and B cell lines. CD40-mediated CD40L expression was inhibited by a protein tyrosine kinase inhibitor, herbimycin, in a dose-dependent manner, suggesting that the induction of CD40L expression via CD40 requires protein tyrosine kinase activity. CD40L surface expression correlated with constitutive or inducible levels of CD40L-specific mRNA, as determined by reverse transcribed PCR analysis (RT-PCR) using CD40L-homologous primers. Furthermore, CD40L on DCs was functional, since CD40L+ DCs, unlike CD40L- DCs, induced B cell IgG and IgA production, and this induction could be inhibited by blocking CD40L-CD40 interactions with mAb to CD40L. Thus, CD40L on DCs and CD40L induced by crosslinking CD40 may regulate B cell activation and maturation. The importance of DC CD40L expression on B cell function is discussed.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1767 1550-6606
DOI:	10.4049/jimmunol.157.10.4363