Search Results - "Norman, Thomas M"

Stochastic Switching of Cell Fate in Microbes by Norman, Thomas M, Lord, Nathan D, Paulsson, Johan, Losick, Richard

“…Microbes transiently differentiate into distinct, specialized cell types to generate functional diversity and cope with changing environmental conditions…”
Get full text

Exploring genetic interaction manifolds constructed from rich single-cell phenotypes by Norman, Thomas M, Horlbeck, Max A, Replogle, Joseph M, Ge, Alex Y, Xu, Albert, Jost, Marco, Gilbert, Luke A, Weissman, Jonathan S

“…How cellular and organismal complexity emerges from combinatorial expression of genes is a central question in biology. High-content phenotyping approaches…”
Get full text

A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response by Adamson, Britt, Norman, Thomas M., Jost, Marco, Cho, Min Y., Nuñez, James K., Chen, Yuwen, Villalta, Jacqueline E., Gilbert, Luke A., Horlbeck, Max A., Hein, Marco Y., Pak, Ryan A., Gray, Andrew N., Gross, Carol A., Dixit, Atray, Parnas, Oren, Regev, Aviv, Weissman, Jonathan S.

“…Functional genomics efforts face tradeoffs between number of perturbations examined and complexity of phenotypes measured. We bridge this gap with Perturb-seq,…”
Get full text

Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing by Replogle, Joseph M., Norman, Thomas M., Xu, Albert, Hussmann, Jeffrey A., Chen, Jin, Cogan, J. Zachery, Meer, Elliott J., Terry, Jessica M., Riordan, Daniel P., Srinivas, Niranjan, Fiddes, Ian T., Arthur, Joseph G., Alvarado, Luigi J., Pfeiffer, Katherine A., Mikkelsen, Tarjei S., Weissman, Jonathan S., Adamson, Britt

“…Single-cell CRISPR screens enable the exploration of mammalian gene function and genetic regulatory networks. However, use of this technology has been limited…”
Get full text

Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs by Jost, Marco, Santos, Daniel A., Saunders, Reuben A., Horlbeck, Max A., Hawkins, John S., Scaria, Sonia M., Norman, Thomas M., Hussmann, Jeffrey A., Liem, Christina R., Gross, Carol A., Weissman, Jonathan S.

“…A lack of tools to precisely control gene expression has limited our ability to evaluate relationships between expression levels and phenotypes. Here, we…”
Get full text

Stochastic antagonism between two proteins governs a bacterial cell fate switch by Lord, Nathan D, Norman, Thomas M, Yuan, Ruoshi, Bakshi, Somenath, Losick, Richard, Paulsson, Johan

“…Cell fate decision circuits must be variable enough for genetically identical cells to adopt a multitude of fates, yet ensure that these states are distinct,…”
Get full text

Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors by Replogle, Joseph M, Bonnar, Jessica L, Pogson, Angela N, Liem, Christina R, Maier, Nolan K, Ding, Yufang, Russell, Baylee J, Wang, Xingren, Leng, Kun, Guna, Alina, Norman, Thomas M, Pak, Ryan A, Ramos, Daniel M, Ward, Michael E, Gilbert, Luke A, Kampmann, Martin, Weissman, Jonathan S, Jost, Marco

“…CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial…”
Get full text

Broadly heterogeneous activation of the master regulator for sporulation in Bacillus subtilis by Chastanet, Arnaud, Vitkup, Dennis, Yuan, Guo-Cheng, Norman, Thomas M, Liu, Jun S, Losick, Richard M

“…A model system for investigating how developmental regulatory networks determine cell fate is spore formation in Bacillus subtilis. The master regulator for…”
Get full text

Exploiting Natural Fluctuations to Identify Kinetic Mechanisms in Sparsely Characterized Systems by Hilfinger, Andreas, Norman, Thomas M, Paulsson, Johan

“…From biochemistry to ecology, many biological systems are stochastic, complex, and sparsely characterized. In such systems, each component may respond to…”
Get more information

Memory and modularity in cell-fate decision making by Norman, Thomas M., Lord, Nathan D., Paulsson, Johan, Losick, Richard

“…Genetically identical cells sharing an environment can display markedly different phenotypes. It is often unclear how much of this variation derives from…”
Get full text

Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens by Dixit, Atray, Parnas, Oren, Li, Biyu, Chen, Jenny, Fulco, Charles P., Jerby-Arnon, Livnat, Marjanovic, Nemanja D., Dionne, Danielle, Burks, Tyler, Raychowdhury, Raktima, Adamson, Britt, Norman, Thomas M., Lander, Eric S., Weissman, Jonathan S., Friedman, Nir, Regev, Aviv

“…Genetic screens help infer gene function in mammalian cells, but it has remained difficult to assay complex phenotypes—such as transcriptional profiles—at…”
Get full text

Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq by Replogle, Joseph M, Saunders, Reuben A, Pogson, Angela N, Hussmann, Jeffrey A, Lenail, Alexander, Guna, Alina, Mascibroda, Lauren, Wagner, Eric J, Adelman, Karen, Lithwick-Yanai, Gila, Iremadze, Nika, Oberstrass, Florian, Lipson, Doron, Bonnar, Jessica L, Jost, Marco, Norman, Thomas M, Weissman, Jonathan S

“…A central goal of genetics is to define the relationships between genotypes and phenotypes. High-content phenotypic screens such as Perturb-seq (CRISPR-based…”
Get full text

Molecular recording of mammalian embryogenesis by Chan, Michelle M., Smith, Zachary D., Grosswendt, Stefanie, Kretzmer, Helene, Norman, Thomas M., Adamson, Britt, Jost, Marco, Quinn, Jeffrey J., Yang, Dian, Jones, Matthew G., Khodaverdian, Alex, Yosef, Nir, Meissner, Alexander, Weissman, Jonathan S.

“…Ontogeny describes the emergence of complex multicellular organisms from single totipotent cells. This field is particularly challenging in mammals, owing to…”
Get full text

Constraints on Fluctuations in Sparsely Characterized Biological Systems by Hilfinger, Andreas, Norman, Thomas M, Vinnicombe, Glenn, Paulsson, Johan

“…Biochemical processes are inherently stochastic, creating molecular fluctuations in otherwise identical cells. Such "noise" is widespread but has proven…”
Get full text

Drivers of heterogeneity in synovial fibroblasts in rheumatoid arthritis by Smith, Melanie H., Gao, Vianne R., Periyakoil, Preethi K., Kochen, Alejandro, DiCarlo, Edward F., Goodman, Susan M., Norman, Thomas M., Donlin, Laura T., Leslie, Christina S., Rudensky, Alexander Y.

“…Inflammation of non-barrier immunologically quiescent tissues is associated with a massive influx of blood-borne innate and adaptive immune cells. Cues from…”
Get full text

EMG AND TMD by Jenkins, Dan, DDS, Thomas, Norman, BDS, MB, BSc, PhD

Get full text