Search Results - "Nordlund, H. R"

Genetically engineered avidins and streptavidins by Laitinen, O. H, Hytönen, V. P, Nordlund, H. R, Kulomaa, M. S

“…Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification…”
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Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli by Hytönen, Vesa P, Laitinen, Olli H, Airenne, Tomi T, Kidron, Heidi, Meltola, Niko J, Porkka, Eevaleena J, Hörhä, Jarno, Paldanius, Tiina, Määttä, Juha A E, Nordlund, Henri R, Johnson, Mark S, Salminen, Tiina A, Airenne, Kari J, Ylä-Herttuala, Seppo, Kulomaa, Markku S

“…Chicken avidin is a highly popular tool with countless applications in the life sciences. In the present study, an efficient method for producing avidin…”
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Chicken Avidin-related Protein 4/5 Shows Superior Thermal Stability when Compared with Avidin while Retaining High Affinity to Biotin by Hytönen, Vesa P., Nyholm, Thomas K.M., Pentikäinen, Olli T., Vaarno, Jonne, Porkka, Eevaleena J., Nordlund, Henri R., Johnson, Mark S., Slotte, J. Peter, Laitinen, Olli H., Kulomaa, Markku S.

“…The protein chicken avidin is a commonly used tool in various applications. The avidin gene belongs to a gene family that also includes seven other members…”
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Production of Hev b5 as a fluorescent biotin-binding tripartite fusion protein in insect cells by Nordlund, Henri R., Laitinen, Olli H., Uotila, Sanna T.H., Kulmala, Minna, Kalkkinen, Nisse, Kulomaa, Markku S.

“…The presented green fluorescent protein and streptavidin core-based tripartite fusion system provides a simple and efficient way for the production of proteins…”
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Brave new (strept)avidins in biotechnology by Laitinen, Olli H, Nordlund, Henri R, Hytönen, Vesa P, Kulomaa, Markku S

“…Avidin and streptavidin are widely used in (strept)avidin–biotin technology, which is based on their tight biotin-binding capability. These techniques are…”
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Crystal Structure of Rhizavidin: Insights into the Enigmatic High-Affinity Interaction of an Innate Biotin-Binding Protein Dimer by Meir, Amit, Helppolainen, Satu H., Podoly, Erez, Nordlund, Henri R., Hytönen, Vesa P., Määttä, Juha A., Wilchek, Meir, Bayer, Edward A., Kulomaa, Markku S., Livnah, Oded

“…Rhizavidin, from the proteobacterium Rhizobium etli, exhibits high affinity towards biotin but maintains an inherent dimeric quaternary structure and thus,…”
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Baculovirus capsid display: a novel tool for transduction imaging by Kukkonen, Sari P., Airenne, Kari J., Marjomäki, Varpu, Laitinen, Olli H., Lehtolainen, Pauliina, Kankaanpää, Pasi, Mähönen, Anssi J., Räty, Jani K., Nordlund, Henri R., Oker-Blom, Christian, Kulomaa, Markku S., Ylä-Herttuala, Seppo

“…Baculoviruses are enveloped insect viruses that can carry large quantities of foreign DNA in their genome. Baculoviruses have proved to be very promising gene…”
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Characterization of poultry egg-white avidins and their potential as a tool in pretargeting cancer treatment by Hytönen, Vesa P, Laitinen, Olli H, Grapputo, Alessandro, Kettunen, Anu, Savolainen, Janne, Kalkkinen, Nisse, Marttila, Ari T, Nordlund, Henri R, Nyholm, Thomas K M, Paganelli, Giovanni, Kulomaa, Markku S

“…Chicken avidin and bacterial streptavidin are proteins used in a wide variety of applications in the life sciences due to their strong affinity for biotin. A…”
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Design and Construction of Highly Stable, Protease-resistant Chimeric Avidins by Hytönen, Vesa P., Määttä, Juha A.E., Nyholm, Thomas K.M., Livnah, Oded, Eisenberg-Domovich, Yael, Hyre, David, Nordlund, Henri R., Hörhä, Jarno, Niskanen, Einari A., Paldanius, Tiina, Kulomaa, Tuomas, Porkka, Eevaleena J., Stayton, Patrick S., Laitinen, Olli H., Kulomaa, Markku S.

“…The chicken avidin gene family consists of avidin and seven separate avidin-related genes (AVRs) 1–7. Avidin protein is a widely used biochemical tool, whereas…”
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Construction of a Dual Chain Pseudotetrameric Chicken Avidin by Combining Two Circularly Permuted Avidins by Nordlund, Henri R, Laitinen, Olli H, Hytönen, Vesa P, Uotila, Sanna T H, Porkka, Eevaleena, Kulomaa, Markku S

“…Two distinct circularly permuted forms of chicken avidin were designed with the aim of constructing a fusion avidin containing two biotin-binding sites in one…”
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Tetravalent single-chain avidin: from subunits to protein domains via circularly permuted avidins by Nordlund, Henri R, Hytönen, Vesa P, Hörhä, Jarno, Määttä, Juha A E, White, Daniel J, Halling, Katrin, Porkka, Eevaleena J, Slotte, J Peter, Laitinen, Olli H, Kulomaa, Markku S

“…scAvd (single-chain avidin, where two dcAvd are joined in a single polypeptide chain), having four biotin-binding domains, was constructed by fusion of…”
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Rational Design of an Active Avidin Monomer by Laitinen, Olli H., Nordlund, Henri R., Hytönen, Vesa P., Uotila, Sanna T.H., Marttila, Ari T., Savolainen, Janne, Airenne, Kari J., Livnah, Oded, Bayer, Edward A., Wilchek, Meir, Kulomaa, Markku S.

“…Homotetrameric chicken avidin that binds four molecules of biotin was converted to a monomeric form (monoavidin) by mutations of two interface residues:…”
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A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo by Laitinen, Olli H., Airenne, Kari J., Hytönen, Vesa P., Peltomaa, Erik, Mähönen, Anssi J., Wirth, Thomas, Lind, Miia M., Mäkelä, Kari A., Toivanen, Pyry I., Schenkwein, Diana, Heikura, Tommi, Nordlund, Henri R., Kulomaa, Markku S., Ylä-Herttuala, Seppo

“…We have constructed a novel tetra-promoter vector (pBVboostFG) system that enables screening of gene/cDNA libraries for functional genomic studies. The vector…”
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Dual-affinity avidin molecules by Hytönen, Vesa P., Nordlund, Henri R., Hörhä, Jarno, Nyholm, Thomas K. M., Hyre, David E., Kulomaa, Tuomas, Porkka, Eevaleena J., Marttila, Ari T., Stayton, Patrick S., Laitinen, Olli H., Kulomaa, Markku S.

“…A recently reported dual‐chain avidin was modified further to contain two distinct, independent types of ligand‐binding sites within a single polypeptide…”
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Introduction of histidine residues into avidin subunit interfaces allows pH-dependent regulation of quaternary structure and biotin binding by Nordlund, Henri R, Hytönen, Vesa P, Laitinen, Olli H, Uotila, Sanna T.H, Niskanen, Einari A, Savolainen, Janne, Porkka, Eevaleena, Kulomaa, Markku S

“…In order to turn the subunit association and biotin binding of avidin into pH-sensitive phenomena, we have replaced individually three amino acid residues in…”
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Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin by Laitinen, Olli H, Hytönen, Vesa P, Ahlroth, Mervi K, Pentikäinen, Olli T, Gallagher, Ciara, Nordlund, Henri R, Ovod, Vladimir, Marttila, Ari T, Porkka, Eevaleena, Heino, Sanna, Johnson, Mark S, Airenne, Kari J, Kulomaa, Markku S

“…Chicken avidin and bacterial streptavidin are proteins familiar from their use in various (strept)avidin-biotin technological applications. Avidin binds the…”
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Enhancing the thermal stability of avidin. Introduction of disulfide bridges between subunit interfaces by Nordlund, Henri R, Laitinen, Olli H, Uotila, Sanna T H, Nyholm, Thomas, Hytönen, Vesa P, Slotte, J Peter, Kulomaa, Markku S

“…In this study we showed that tetrameric chicken avidin can be stabilized by introducing intermonomeric disulfide bridges between its subunits. These covalent…”
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Novel Avidin-like Protein from a Root Nodule Symbiotic Bacterium, Bradyrhizobium japonicum by Nordlund, Henri R., Hytönen, Vesa P., Laitinen, Olli H., Kulomaa, Markku S.

“…Bradyrhizobium japonicum is an important nitrogenfixing symbiotic bacterium, which can form root nodules on soybeans. These bacteria have a gene encoding a…”
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Chicken genome analysis reveals novel genes encoding biotin-binding proteins related to avidin family by Niskanen, Einari A, Hytönen, Vesa P, Grapputo, Alessandro, Nordlund, Henri R, Kulomaa, Markku S, Laitinen, Olli H

“…A chicken egg contains several biotin-binding proteins (BBPs), whose complete DNA and amino acid sequences are not known. In order to identify and characterise…”
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