Search Results - "Nor Hafizah Ahmad Kamarudin"

The Role of Surface Exposed Lysine in Conformational Stability and Functional Properties of Lipase from Staphylococcus Family by Ahmad, Nurul Nadirah, Ahmad Kamarudin, Nor Hafizah, Leow, Adam Thean Chor, Rahman, Raja Noor Zaliha Raja Abd

“…Surface charge residues have been recognized as one of the stability determinants in protein. In this study, we sought to compare and analyse the stability and…”
Get full text

Ion-Pair Interaction and Hydrogen Bonds as Main Features of Protein Thermostability in Mutated T1 Recombinant Lipase Originating from Geobacillus zalihae by Ishak, Siti Nor Hasmah, Kamarudin, Nor Hafizah Ahmad, Ali, Mohd Shukuri Mohamad, Leow, Adam Thean Chor, Rahman, Raja Noor Zaliha Raja Abd

“…A comparative structure analysis between space- and an Earth-grown T1 recombinant lipase from had shown changes in the formation of hydrogen bonds and ion-pair…”
Get full text

The Role of Solvent-Accessible Leu-208 of Cold-Active Pseudomonas fluorescens Strain AMS8 Lipase in Interfacial Activation, Substrate Accessibility and Low-Molecular Weight Esterification in the Presence of Toluene by Yaacob, Norhayati, Ahmad Kamarudin, Nor Hafizah, Leow, Adam Thean Chor, Salleh, Abu Bakar, Raja Abd Rahman, Raja Noor Zaliha, Mohamad Ali, Mohd Shukuri

“…The alkaline cold-active lipase from AMS8 undergoes major structural changes when reacted with hydrophobic organic solvents. In toluene, the AMS8 lipase…”
Get full text

Directed Evolution of Recombinant C-Terminal Truncated Staphylococcus epidermidis Lipase AT2 for the Enhancement of Thermostability by Veno, Jiivittha, Ahmad Kamarudin, Nor Hafizah, Mohamad Ali, Mohd Shukuri, Masomian, Malihe, Raja Abd Rahman, Raja Noor Zaliha

“…In the industrial processes, lipases are expected to operate at temperatures above 45 °C and could retain activity in organic solvents. Hence, a C-terminal…”
Get full text

Effects of Lid 1 Mutagenesis on Lid Displacement, Catalytic Performances and Thermostability of Cold-active Pseudomonas AMS8 Lipase in Toluene by Yaacob, Norhayati, Ahmad Kamarudin, Nor Hafizah, Leow, Adam Thean Chor, Salleh, Abu Bakar, Rahman, Raja Noor Zaliha Raja Abd, Ali, Mohd Shukuri Mohamad

“…Pseudomonas fluorescens AMS8 lipase lid 1 structure is rigid and holds unclear roles due to the absence of solvent-interactions. Lid 1 region was stabilized by…”
Get full text

Expression of an organic solvent stable lipase from Staphylococcus epidermidis AT2 by Rahman, Raja Noor Zaliha Raja Abd, Kamarudin, Nor Hafizah Ahmad, Yunus, Jalimah, Salleh, Abu Bakar, Basri, Mahiran

“…An organic solvent tolerant lipase gene from Staphylococcus epidermidis AT2 was successfully cloned and expressed with pTrcHis2 in E. coli TOP10. Sequence…”
Get full text

An integrated overview of bacterial carboxylesterase: Structure, function and biocatalytic applications by Johan, Ummie Umaiera Mohd, Rahman, Raja Noor Zaliha Raja Abd, Kamarudin, Nor Hafizah Ahmad, Ali, Mohd Shukuri Mohamad

“…[Display omitted] •Classification of carboxylesterase, family I-XIX.•An overview of 3D structure bacterial carboxylesterase.•Catalytic mechanism of bacterial…”
Get full text

Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues by Matinja, Adamu Idris, Kamarudin, Nor Hafizah Ahmad, Leow, Adam Thean Chor, Oslan, Siti Nurbaya, Ali, Mohd Shukuri Mohamad

“…Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth's biosphere. One of the…”
Get full text

Structure elucidation and docking analysis of 5M mutant of T1 lipase Geobacillus zalihae by Ishak, Siti Nor Hasmah, Kamarudin, Nor Hafizah Ahmad, Ali, Mohd Shukuri Mohamad, Leow, Adam Thean Chor, Shariff, Fairolniza Mohd, Rahman, Raja Noor Zaliha Raja Abd

“…5M mutant lipase was derived through cumulative mutagenesis of amino acid residues (D43E/T118N/E226D/E250L/N304E) of T1 lipase from Geobacillus zalihae. A…”
Get full text

Insight into Improved Thermostability of Cold-Adapted Staphylococcal Lipase by Glycine to Cysteine Mutation by Veno, Jiivittha, Rahman, Raja Noor Zaliha Raja Abd, Masomian, Malihe, Ali, Mohd Shukuri Mohamad, Kamarudin, Nor Hafizah Ahmad

“…Thermostability remains one of the most desirable traits in many lipases. Numerous studies have revealed promising strategies to improve thermostability and…”
Get full text

Effect of cysteine mutation at Ca2+ coordinating residues to the autolysis, folding and hydrophobicity of full length and mature Rand protease: molecular dynamics simulation and essential dynamics by Mohd Azrin, Nur Aliyah, Mohamad Ali, Mohd Shukuri, Raja Abd Rahman, Raja Noor Zaliha, Mohd Shariff, Fairolniza, Ahmad Kamarudin, Nor Hafizah, Muhd Noor, Noor Dina

“…Rand protease is a serine protease that shared common characteristics with members of the MEROPS S8 subtilisin family. It is thermostable, highly stable in…”
Get full text

Changes of Thermostability, Organic Solvent, and pH Stability in Geobacillus zalihae HT1 and Its Mutant by Calcium Ion by Ishak, Siti Nor Hasmah, Masomian, Malihe, Kamarudin, Nor Hafizah Ahmad, Ali, Mohd Shukuri Mohamad, Leow, Thean Chor, Rahman, Raja Noor Zaliha Raja Abd

“…Thermostable T1 lipase from has been crystallized using counter-diffusion method under space and Earth conditions. The comparison of the three-dimensional…”
Get full text

Characterization of Carboxylic Acid Reductase from Mycobacterium phlei Immobilized onto Seplite LX120 by Basri, Rose Syuhada, Rahman, Raja Noor Zaliha Raja Abd, Kamarudin, Nor Hafizah Ahmad, Latip, Wahhida, Ali, Mohd Shukuri Mohamad

“…A multi-domain oxidoreductase, carboxylic acid reductase (CAR), can catalyze the one-step reduction of carboxylic acid to aldehyde. This study aimed to…”
Get full text

Stability Enhancement of Aldehyde Dehydrogenase from Anoxybacillus geothermalis Strain D9 Immobilized onto Seplite LX120 by Wahhida Latip, Nur Ezzati Rosli, Mohd Shukuri Mohamad Ali, Nor Hafizah Ahmad Kamarudin, Raja Noor Zaliha Raja Abd Rahman

“…Enzyme stability is regarded as an important criterion for an industrial biocatalyst. Aldehyde dehydrogenase (ALDH) from A. geothermalis strain D9 was…”
Get full text

Immobilization of Hyperthermostable Carboxylesterase EstD9 from Anoxybacillus geothermalis D9 onto Polymer Material and Its Physicochemical Properties by Johan, Ummie Umaiera Mohd, Rahman, Raja Noor Zaliha Raja Abd, Kamarudin, Nor Hafizah Ahmad, Latip, Wahhida, Ali, Mohd Shukuri Mohamad

“…Carboxylesterase has much to offer in the context of environmentally friendly and sustainable alternatives. However, due to the unstable properties of the…”
Get full text

Molecular Dynamic Simulation of Space and Earth-Grown Crystal Structures of Thermostable T1 Lipase Geobacillus zalihae Revealed a Better Structure by Ishak, Siti Nor Hasmah, Aris, Sayangku Nor Ariati Mohamad, Halim, Khairul Bariyyah Abd, Ali, Mohd Shukuri Mohamad, Leow, Thean Chor, Kamarudin, Nor Hafizah Ahmad, Masomian, Malihe, Rahman, Raja Noor Zaliha Raja Abd

“…Less sedimentation and convection in a microgravity environment has become a well-suited condition for growing high quality protein crystals. Thermostable T1…”
Get full text

The Effect of N-Terminal Domain Removal towards the Biochemical and Structural Features of a Thermotolerant Lipase from an Antarctic Pseudomonas sp. Strain AMS3 by Latip, Wahhida, Raja Abd Rahman, Raja Noor Zaliha, Leow, Adam Thean Chor, Mohd Shariff, Fairolniza, Kamarudin, Nor Hafizah Ahmad, Mohamad Ali, Mohd Shukuri

“…Lipase plays an important role in industrial and biotechnological applications. Lipases have been subject to modification at the N and C terminals, allowing…”
Get full text

Structure Prediction and Characterization of Thermostable Aldehyde Dehydrogenase from Newly Isolated Anoxybacillus geothermalis Strain D9 by Rosli, Nur Ezzati, Ali, Mohd Shukuri Mohamad, Kamarudin, Nor Hafizah Ahmad, Masomian, Malihe, Latip, Wahhida, Saadon, Shazleen, Rahman, Raja Noor Zaliha Raja Abd

“…In nature, aldehyde dehydrogenase (ALDH) is widely distributed and mainly involved in the oxidation of aldehydes. Thermostability is one of the key features…”
Get full text

X-ray crystallography of mutant GDSL esterase S12A of Photobacterium marinum J15 by Rahman, Nor Najihah Abdul, Sharif, Fairolniza Mohd, Kamarudin, Nor Hafizah Ahmad, Ali, Mohd Shukuri Mohamad, Aris, Sayangku Nor Ariati Mohamad, Jonet, Mohd Anuar, Rahman, Raja Noor Zaliha Raja Abd, Sabri, Suriana, Leow, Thean Chor

“…GDSL esterase is designated as a member of Family II of lipolytic enzymes known to catalyse the synthesis and hydrolysis of ester bonds. The enzyme possesses a…”
Get full text

Unscrambling the Effect of C-Terminal Tail Deletion on the Stability of a Cold-Adapted, Organic Solvent Stable Lipase from Staphylococcus epidermidis AT2 by Kamarudin, Nor Hafizah Ahmad, Rahman, Raja Noor Zaliha Raja Abd, Ali, Mohd Shukuri Mohamad, Leow, Thean Chor, Basri, Mahiran, Salleh, Abu Bakar

“…Terminal moieties of most proteins are long known to be disordered and flexible. To unravel the functional role of these regions on the structural stability…”
Get full text