Efficacies of DNA and Protein‐Based Gamma‐glutamyl cysteine synthetase (γGCS) Vaccines from Leishmania donovani on the Cutaneous Lesions in Leishmania major infected Balb/c Mice
Gamma‐glutamyl cysteine synthetase (GCS) is an enzyme that catalyses the rate limiting step in glutathione synthesis. In this study the efficacies of the DNA and protein vaccines of GCS from L. donovani was evaluated against cutaneous leishmaniasis caused by L. major. Balb/c mice immunized with plas...
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| Published in: | The FASEB journal Vol. 24; no. S1; p. 450.1 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Federation of American Societies for Experimental Biology
01-04-2010
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| Online Access: | Get full text |
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| Summary: | Gamma‐glutamyl cysteine synthetase (GCS) is an enzyme that catalyses the rate limiting step in glutathione synthesis. In this study the efficacies of the DNA and protein vaccines of GCS from L. donovani was evaluated against cutaneous leishmaniasis caused by L. major. Balb/c mice immunized with plasmid containing the full gene sequence for GCS (pVAX‐GCS) or plasmid alone (pVAX) or the recombinant protein (rGCS) or PBS (control) were challenged with high dose of L. major promastigotes in the shaven rump and the degree of protection was evaluated. by lesion progression, histopathological outcome of lesions and immunological profile. Vaccination of mice with the recombinant protein resulted in protection as demonstrated in the delay in lesion expansion and accompanied by an improved histopathological outcome of the skin lesions with little tissue destruction compared to the control. Furthermore, immunological analysis of the antibody isotypes showed high levels of IgG2a compared to IgG1 which is suggestive of a TH1 immune response and also characterized by the induction of significantly high levels of gamma interferon production with little or no IL‐4 production. Vaccination with DNA plasmid or the plasmid alone did not confer any protection. These results demonstrate that the recombinant protein of γGCS from L. donovani induced cross immunoprotection with improved histopathological outcome against cutaneous leishmaniasis suggesting that it represents a good candidate for use as a vaccine.
Grant Funding Source: Carnegie Corperation/ABU Fellowship Award |
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| ISSN: | 0892-6638 1530-6860 |
| DOI: | 10.1096/fasebj.24.1_supplement.450.1 |