SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro

Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time rever...

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Bibliographic Details
Published in:	Memórias do Instituto Oswaldo Cruz Vol. 115; p. e200200
Main Authors:	Cipitelli, Marcio da Costa, Valentin, Elizabeth, da Cruz, Nadia Vaez Gonçalves, Nogueira, Tatiana Ls, de Melo, Elaine Cristina Amaro, da Silva, Rebeca Araujo, Serra, Marcelo M, Meriano, André L, Colares, Alberto Ml, Dornelas-Ribeiro, Marcos, Santos, Caleb Gm
Format:	Journal Article
Language:	English
Published:	Brazil Instituto Oswaldo Cruz, Ministério da Saúde 01-01-2020 Fundação Oswaldo Cruz (FIOCRUZ)
Subjects:	Betacoronavirus - genetics Betacoronavirus - isolation & purification Brazil - epidemiology coronavirus Coronavirus Infections - diagnosis Coronavirus Infections - epidemiology COVID-19 global pandemic Humans Military Facilities Pandemics PARASITOLOGY Pneumonia, Viral - diagnosis Pneumonia, Viral - epidemiology Reverse Transcriptase Polymerase Chain Reaction RT-PCR SARS-CoV-2 Short Communication TROPICAL MEDICINE Brazil COVID-19 coronavirus SARS-CoV-2 RT-PCR global pandemic
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Summary:	Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charité protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 MD-R and CGMS - Conceptualisation; MCC , NVGC, TLSN, EV, EA and CGMS - formal analysis; MD-R, CGMS, MMS, ALM and AMLC - funding acquisition; RS, EA, EV and CGMS - investigation; CGMS - methodology; MCC , NVGC, TLSN, EV, MD-R, MMS, ALM and AMLC - project administration; MMS, ALM, AMLC, EA and MD-R - resources; RS and CGMS - software; NVGC, TLSN and MD-R - supervision; MCC and CGMS - writing-original draft; MCC and CGMS -writing-review and editing. The authors declare no conflicts of interest, financial or otherwise. MD-R and CGMS contributed equally to this work.
ISSN:	0074-0276 1678-8060 1678-8060
DOI:	10.1590/0074-02760200200