Isolation and sequencing of Orthoflavivirus ilheusense from mosquitoes collected in the Brazilian cerrado, West-Central region, Brazil

Isolation and sequencing of Orthoflavivirus ilheusense from mosquitoes collected in the Brazilian cerrado, West-Central region, Brazil

•Isolation of Orthoflavivirus ilheussense (ILHV) in Psorophora (Janthinosoma) ferox collected in cerrado biome of Brazil.•This is the first report of the circulation of ILHV in the state of Goiás, an arbovirus associated with febrile illness, which is often accompanied by encephalitis.•The strain is...

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Published in:Acta tropica Vol. 257; p. 107272
Main Authors: Reis, Lúcia Aline Moura, Nunes Neto, Joaquim Pinto, Nascimento, Bruna Laís Sena do, Araújo, Alexandre Azevedo de, Nogueira, Cristiano Gomes, Cavalcante, Karina Ribeiro Leite Jardim, Ramos, Daniel Garkauskas, Durans, Darlene de Brito Simith, Wanzeler, Ana Lucia Monteiro, Santos, Maissa Maia, Júnior, José Wilson Rosa, Gonçalves, Ercília de Jesus, Silva, Sandro Patroca da, Cruz, Ana Cecília Ribeiro, Silva, Eliana Vieira Pinto da
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-09-2024
Subjects:
Animals
Arbovirus
Brazil
Chlorocebus aethiops
Culicidae - virology
Disease vectors
Epidemiological monitoring
Flavivirus - classification
Flavivirus - genetics
Flavivirus - isolation & purification
Mosquito vectors
Mosquito Vectors - virology
Orthoflavivirus ilheusense
Phylogeny
Vero Cells
Zoonosis surveillance
Brazil
Orthoflavivirus ilheusense
Disease vectors
Zoonosis surveillance
Arbovirus
Mosquito vectors
Epidemiological monitoring
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Summary:•Isolation of Orthoflavivirus ilheussense (ILHV) in Psorophora (Janthinosoma) ferox collected in cerrado biome of Brazil.•This is the first report of the circulation of ILHV in the state of Goiás, an arbovirus associated with febrile illness, which is often accompanied by encephalitis.•The strain is closely related to ILHV isolated in Belém (PA) during the dry season.•The ILHV detection in various regions from Brazil indicates the risk of the emergence of new diseases. The Orthoflavivirus ilheusense (ILHV) is an arbovirus that was first isolated in Brazil in 1944 during an epidemiologic investigation of yellow fever. Is a member of the Flaviviridae family and it belongs to the antigenic complex of the Ntaya virus group. Psorophora ferox is the primary vector of ILHV and this study presents the isolation and phylogenetic analysis of ILHV in a pool of Ps. ferox collected in the state of Goiás in 2021. Viral isolation tests were performed on Vero cells and C6/36 clones. The indirect immunofluorescence test (IFI) was used to confirm the positivity of the sample. The positive sample underwent RT-qPCR, sequencing, and phylogenetic analysis. This is the first report of ILHV circulation in this municipality and presented close relationship between this isolate and another ILHV isolate collected in the city of Belém (PA). [Display omitted]
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ISSN:0001-706X
1873-6254
1873-6254
DOI:10.1016/j.actatropica.2024.107272