Lipoprotein lipase hydrolysis products induce pro-inflammatory cytokine expression in triple-negative breast cancer cells

Lipoprotein lipase hydrolysis products induce pro-inflammatory cytokine expression in triple-negative breast cancer cells

Breast cancer cell growth and proliferation requires lipids for energy production, cell membrane synthesis, or as signaling molecules. Lipids can be delivered to cells by lipoprotein lipase (LPL), an extracellular lipase that hydrolyzes triacylglycerols and phospholipids from lipoproteins, that is e...

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Bibliographic Details
Published in:BMC research notes Vol. 14; no. 1; pp. 1 - 315
Main Authors: Tobin, Alexandria J, Noel, Nicholas P, Christian, Sherri L, Brown, Robert J
Format: Journal Article
Language:English
Published: London BioMed Central Ltd 17-08-2021
BioMed Central
BMC
Subjects:
Adipose tissue
Angiogenesis
Antibody arrays
Arrays
Breast cancer
Cell membranes
Cell proliferation
Cell viability
Cytokines
Development and progression
Diagnosis
Endothelial cells
Gene expression
Health aspects
Hydrolysis
Inflammation
Interleukin 4
Interleukin 6
Lipase
Lipids
Lipoprotein lipase
Lipoproteins
Medical prognosis
Metabolic activity
Metabolism
Metastasis
Phospholipids
Research Note
Tumor cell lines
Tumor microenvironment
Tumor necrosis factor-TNF
Tumor necrosis factor-α
Canada
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Summary:Breast cancer cell growth and proliferation requires lipids for energy production, cell membrane synthesis, or as signaling molecules. Lipids can be delivered to cells by lipoprotein lipase (LPL), an extracellular lipase that hydrolyzes triacylglycerols and phospholipids from lipoproteins, that is expressed by adipose tissue and some breast cancer cell lines. Studies have shown that lipoprotein hydrolysis products induce pro-inflammatory cytokine secretion by endothelial cells. Thus, our objective was to determine if hydrolysis products generated by LPL from total lipoproteins can also promote pro-inflammatory cytokine secretion from breast cancer cells. Using cytokine arrays, we found that MDA-MB-231 cells increased secretion of seven cytokines in response to treatment with lipoprotein hydrolysis products. In contrast, MCF-7 cells showed decreased secretion of two cytokines. Expanding the analysis to additional cell lines by ELISA, we found increased secretion of TNF-[alpha] and IL-6 by MDA-MB-468 cells, and increased secretion of IL-4 by MDA-MB-468 and SKBR3 cells. The changes to cytokine secretion profiles of the breast cancer cell types examined, including the non-cancerous MCF-10a breast cells, were independent of increased cell metabolic activity. These results provide information on how lipoprotein hydrolysis products within the tumor microenvironment might affect breast cancer cell viability and progression.
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ISSN:1756-0500
1756-0500
DOI:10.1186/s13104-021-05728-z