DNA:DNA hybridization and chemotaxonomic studies of Thermus scotoductus

The species Thermus scotoductus was recently described as containing several non-pigmented isolates from Selfoss, Iceland, and the X-1 strain from the USA (Kristjansson et al., 1994). In this study, we performed DNA:DNA hybridizations and chemotaxonomic studies on several non-pigmented Thermus isola...

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Bibliographic Details
Published in:	Research in microbiology Vol. 146; no. 4; pp. 315 - 324
Main Authors:	Tenreiro, S, Nobre, M.F, Hoste, B, Gillis, M, Kristjansson, J.K, Da Costa, M.S
Format:	Journal Article
Language:	English
Published:	Paris Elsevier SAS 1995 Elsevier
Subjects:	ADN, Acide gras Pigment, Thermus scotoductus, Thermus Bacteriology Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Chromatography, Thin Layer Composition, DNA homology, Taxonomy, Phylogenetics, Geothermal impact Composition, Homologie, ADN, Taxonomie, Phylogénétique, Impact géothermal DNA, Bacterial - analysis DNA, Bacterial - chemistry DNA, Fatty acid, Pigment, Thermus, Thermus scotoductus Fatty Acids - chemistry Fundamental and applied biological sciences. Psychology In Vitro Techniques Isolation and description Lipids - chemistry Microbiology Mission oriented research Nucleic Acid Hybridization Systematics Thermus - chemistry Thermus - classification Thermus - genetics Thermus scotoductus ADN, Acide gras Pigment, Thermus scotoductus, Thermus Composition, Homologie, ADN, Taxonomie, Phylogénétique, Impact géothermal Composition, DNA homology, Taxonomy, Phylogenetics, Geothermal impact DNA, Fatty acid, Pigment, Thermus, Thermus scotoductus Thermus Composition Taxonomy DNA Chemotaxonomy Pigments Bacteria Genotype Lipids Phylogeny Fatty acids
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Summary:	The species Thermus scotoductus was recently described as containing several non-pigmented isolates from Selfoss, Iceland, and the X-1 strain from the USA (Kristjansson et al., 1994). In this study, we performed DNA:DNA hybridizations and chemotaxonomic studies on several non-pigmented Thermus isolates from other geographical areas to assess their relationship to the strains originally assigned to this species. The results of DNA:DNA hybridizations showed that strains NH and DI from London and strains VI-7a and VI-13 from Vizela, Portugal, belonged to T. scotoductus. T. scotoductus X-1 (ATCC 27978) was composed of two stable colony types, one of which had a major glycolipid different from the one present in the other colony type and from all other Thermus strains examined as well. The fatty acid composition of the isolates from Selfoss and London were practically identical. However, the fatty acid composition of strain X-1, the individual colony types of this strain and the Vizela strains were different from the Selfoss-London isolates and from each other. Another non-pigmented strain, designated SPS-11, belonged to a different DNA homology group. L'espèce Thermus scotoductus récemment décrite comporte diverses souches non pigmentées, isolées à Selfoss (Islande) et la souche X-1 isolée, aux USA (Kristjansson et al., 1994). Nous avons entrepris des hybridations ADR/ADN et des études chimiotaxonomiques sur plusieurs souches non pigmentées du genre Thermus, isolées d'autres zones géographiques, afin de déterminer leurs relations avec la souche originale de l'espèce T. scotoductus. Les résultats de l'hybridation ADN/ADN montrent que les souches NH et DI de Londres et VI-7a et VI-13 de Vizela (Portugal) appartiennent à cette espèce. La souche X-1 (ATCC 27978) développe deux types de colonies stables, dont l'un possède un glycolipide majeur différent de celui qui est présent dans l'autre type et chez toutes les autres souches de Thermus examinées. La composition des acides gras des souches isolées à Selfoss et à Londres est pratiquement identique. Par ailleurs, les acides gras de la souche X-1 et des souches de Vizela diffèrent de ceux des souches Selfoss-Londres. Une autre souche non-pigmentée (SPS-11) appartient à un groupe d'homologie ADN différent.
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ISSN:	0923-2508 1769-7123
DOI:	10.1016/0923-2508(96)81054-0