Simple, effective and economical explant-surface sterilization protocol for cowpea, rice and sorghum seeds

Three different surface sterilization methods were evaluated using seeds and excised embryos of cowpea, rice and sorghum as explants: Method 1: Ethanol alone in concentrations of 95, 90, 85 and 70% at different time intervals and observed at different days. Method 2: Locally produced bleaching solut...

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Bibliographic Details
Published in:	African journal of biotechnology Vol. 8; no. 20; pp. 5395 - 5399
Main Authors:	Oyebanji, O B, Nweke, O, Odebunmi, O, Galadima, N B, Idris, MS, Nnodi, UN, Afolabi, A S, Ogbadu, G H
Format:	Journal Article
Language:	English
Published:	19-10-2009
Subjects:	Oryza sativa Sorghum
Online Access:	Get full text
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Summary:	Three different surface sterilization methods were evaluated using seeds and excised embryos of cowpea, rice and sorghum as explants: Method 1: Ethanol alone in concentrations of 95, 90, 85 and 70% at different time intervals and observed at different days. Method 2: Locally produced bleaching solution (JIK+ -Reckitt and Benckiser (Nig) Ltd) containing 3.5% Sodium hypochlorite) at different time intervals, observed at different days. Method 3 (The control): The routinely used two step sterilization procedure using 90% ethanol for 3 min followed by sodium hypochlorite 3.5% for 30 min. This is commonly used in most laboratories. However, neat concentration of the locally produced bleaching solution of JIK super(+) (-Reckitt and Benckiser (Nig) Ltd) containing 3.5% sodium hypochlorite was used instead of the pure sodium hypochlorite solution. Our results showed that Method 2 produced the highest reduction in bacterial and fungal contamination (0%) at time intervals between 20 - 45 min. The search for a simple, rapid and economical method of sterilizing explants for tissues culture, instead of the orthodox two-step -two reagent -technique, necessitated these experiments; we would, therefore recommend this technique due to its simplicity and economy.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1684-5315 1684-5315