Hepatoprotective activity of LC–ESI-MS standardized Iris spuria rhizome extract on its main bioactive constituents

The study was designed to evaluate the hepatoprotective activity of Iris spuria against paracetamol induced toxicity at two different doses 100 and 200mg/kg. The extract showed significant protective activity (p>0.01) at both the doses in dose dependent manner. Administration of the plant extract...

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Bibliographic Details
Published in:	Phytomedicine (Stuttgart) Vol. 21; no. 10; pp. 1202 - 1207
Main Authors:	Akther, Nayeema, Andrabi, Khursheed, Nissar, Albeena, Ganaie, Showkat, Chandan, B.K., Gupta, A.P., Khuswant, Manoj, Sultana, Sarwat, Shawl, Abdul S.
Format:	Journal Article
Language:	English
Published:	Germany Elsevier GmbH 15-09-2014 Urban & Fischer Verlag
Subjects:	Acetaminophen - toxicity Animals Biomarkers - metabolism Chemical and Drug Induced Liver Injury - drug therapy Chemical and Drug Induced Liver Injury - physiopathology Chromatography, Liquid Dose-Response Relationship, Drug Drug metabolism Glutathione - drug effects Glutathione - metabolism Health aspects Hepatoprotective Iris (Plant) Iris Plant - chemistry Iris spuria Isoflavonoids Lipid Peroxidation - drug effects Liver Male Materia medica, Vegetable Oxidative Stress - drug effects Paracetamol Physiological aspects Plant extracts Plant Extracts - chemistry Plant Extracts - isolation & purification Plant Extracts - pharmacology Rats Rats, Wistar Rhizome - chemistry Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Tectoridin Hepatoprotective Paracetamol Isoflavonoids Tectoridin Iris spuria
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Summary:	The study was designed to evaluate the hepatoprotective activity of Iris spuria against paracetamol induced toxicity at two different doses 100 and 200mg/kg. The extract showed significant protective activity (p>0.01) at both the doses in dose dependent manner. Administration of the plant extract restored the paracetamol induced elevated levels of serum marker and distorted hepatic tissue architecture. The lipid peroxides (LPO) and glutathione (GSH) levels were also restored towards normal in liver tissue significantly. The main chemical constituents of the extract identified by the liquid chromatography–tandem mass spectrometry (LC–ESI-MSMS) were found to be flavones and isoflavonoids. Tectoridin and iristectorigenin A were the principal compounds present in the methanolic extract of Iris spuria.
ISSN:	0944-7113 1618-095X
DOI:	10.1016/j.phymed.2014.04.007