Angiopoietin 1, PDGF-B, and TGF-β gene regulation in endothelial cell and smooth muscle cell interaction

The vascular wall is mainly composed of endothelial cells (ECs) and smooth muscle cells (SMCs). The crosstalking between these two cell types is critical in the vascular maturation process. Genetic studies suggest that the Tie2/angiopoietin 1 (Ang1) pathway regulates vascular remodeling. However, th...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of cellular biochemistry Vol. 91; no. 3; pp. 584 - 593
Main Authors:	Nishishita, Toshishita, Lin, Pengnian Charles
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 15-02-2004
Subjects:	angiopoietin Angiopoietin-1 - genetics Angiopoietin-1 - metabolism Angiopoietin-1 - pharmacology Blotting, Northern Blotting, Western Cell Communication - physiology Cell Differentiation - physiology Cell Line Down-Regulation Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Enzyme Inhibitors - pharmacology Gene Expression Regulation Humans Intercellular Signaling Peptides and Proteins - genetics Intercellular Signaling Peptides and Proteins - metabolism Intercellular Signaling Peptides and Proteins - pharmacology Mitogen-Activated Protein Kinase Kinases - antagonists & inhibitors Mitogen-Activated Protein Kinase Kinases - metabolism Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism PDGF Pericytes - physiology Phosphatidylinositol 3-Kinases - antagonists & inhibitors Phosphatidylinositol 3-Kinases - metabolism Plasminogen Activator Inhibitor 1 - metabolism Platelet-Derived Growth Factor - genetics Platelet-Derived Growth Factor - metabolism Platelet-Derived Growth Factor - pharmacology Protein Kinase C - antagonists & inhibitors Protein Kinase C - metabolism Proto-Oncogene Proteins c-sis Receptor, TIE-2 - metabolism RNA, Messenger - analysis RNA, Messenger - metabolism Signal Transduction - physiology TGF-β Tie2 Transforming Growth Factor beta - genetics Transforming Growth Factor beta - metabolism Transforming Growth Factor beta - pharmacology Umbilical Veins - cytology Up-Regulation vascular maturation
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The vascular wall is mainly composed of endothelial cells (ECs) and smooth muscle cells (SMCs). The crosstalking between these two cell types is critical in the vascular maturation process. Genetic studies suggest that the Tie2/angiopoietin 1 (Ang1) pathway regulates vascular remodeling. However, the molecular mechanism is unclear. PDGF is a potent chemoattractant for SMCs, and TGF‐β regulates SMC differentiation. Here, we examined gene regulation. PDGF‐B stimulation upregulated Ang1 expression in SMCs through the PI3K and PKC pathways. PDGF‐B stimulation also produced an acute induction of TGF‐β expression in SMCs through the MAPK/ERK pathway. Interestingly, TGF‐β negatively regulated Ang1 expression induced by the PDGF‐B stimulation in SMCs. Reciprocally, we observed that stimulation of ECs with either Ang1 or TGF‐β slightly downregulated PDGF expression. A combination of both TGF‐β with Ang1 produced much stronger downregulation of PDGF. Our data showed complex gene regulations that include both positive and negative regulations between ECs and SMCs to maintain vascular homeostasis. © 2003 Wiley‐Liss, Inc.
Bibliography:	istex:B76DAA56DE084E717E60C6340EEE5AE798D07F08 Vanderbilt-Ingram Cancer Center - No. CA68485 Vanderbilt In Vivo Imaging Center - No. CA 86283 ark:/67375/WNG-LT611D4N-K National Cancer Institute (to PCL) - No. CA87756 Vanderbilt Diabetes Center - No. DK20593 ArticleID:JCB10718 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0730-2312 1097-4644
DOI:	10.1002/jcb.10718