Unfolded protein-independent IRE1 activation contributes to multifaceted developmental processes in Arabidopsis
In Arabidopsis, the and double mutant ( ) is unable to activate cytoplasmic splicing of mRNA and regulated IRE1-dependent decay under ER stress, whereas the mutant does not exhibit severe developmental defects under normal conditions. In this study, we focused on the Arabidopsis gene, whose product...
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| Published in: | Life science alliance Vol. 2; no. 5; p. e201900459 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
Life Science Alliance LLC
01-10-2019
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| Online Access: | Get full text |
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| Summary: | In Arabidopsis, the
and
double mutant (
) is unable to activate cytoplasmic splicing of
mRNA and regulated IRE1-dependent decay under ER stress, whereas the mutant does not exhibit severe developmental defects under normal conditions. In this study, we focused on the Arabidopsis
gene, whose product lacks a sensor domain. We found that the
triple mutant is lethal, and heterozygous
(
/+) mutation in the
mutants resulted in growth defects and reduction of the number of pollen grains. Genetic analysis revealed that
is required for male gametophyte development in the
mutant background. Expression of a mutant form of IRE1B that lacks the luminal sensor domain (ΔLD) complemented a developmental defect in the male gametophyte in
haplotype. In vivo, the ΔLD protein was activated by glycerol treatment that increases the composition of saturated lipid and was able to activate regulated IRE1-dependent decay but not
splicing. These observations suggest that IRE1 contributes to plant development, especially male gametogenesis, using an alternative activation mechanism that bypasses the unfolded protein-sensing luminal domain. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 2575-1077 2575-1077 |
| DOI: | 10.26508/lsa.201900459 |