Search Results - "Nishikawa, Takehiro"
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Liposome display for in vitro selection and evolution of membrane proteins
Published in Nature protocols (01-07-2014)“…This article details a Protocol to achieve directed evolution of membrane proteins by displaying proteins on the surface of liposome membranes and iteratively…”
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Substrata Mechanical Stiffness Can Regulate Adhesion of Viable Bacteria
Published in Biomacromolecules (01-06-2008)“…The competing mechanisms that regulate adhesion of bacteria to surfaces and subsequent biofilm formation remain unclear, though nearly all studies have focused…”
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Construction of a Gene Screening System Using Giant Unilamellar Liposomes and a Fluorescence-Activated Cell Sorter
Published in Analytical chemistry (Washington) (05-06-2012)“…We have constructed a gene screening system composed of an in vitro transcription–translation system encapsulated within giant unilamellar liposomes and a…”
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Fabrication of Honeycomb Film of an Amphiphilic Copolymer at the Air−Water Interface
Published in Langmuir (23-07-2002)“…We report a new fabrication method of a thin polymer film with regular array of micropores (honeycomb film). A honeycomb film was fabricated by depositing a…”
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Nitric Oxide Release in Human Aortic Endothelial Cells Mediated by Delivery of Amphiphilic Polysiloxane Nanoparticles to Caveolae
Published in Biomacromolecules (10-08-2009)“…Microdomains such as lipid raft and caveolae are organized as functional compartments in plasma membrane of cells. In this study, we note the functional…”
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Micropatterns Based on Deformation of a Viscoelastic Honeycomb Mesh
Published in Langmuir (22-07-2003)“…We report that various geometric patterns can be formed upon mechanical deformation of hexagonal micro polymer mesh. The patterning of micromesh can be applied…”
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Directed Evolution of Proteins through In Vitro Protein Synthesis in Liposomes
Published in Journal of Nucleic Acids (01-01-2012)“…Directed evolution of proteins is a technique used to modify protein functions through “Darwinian selection.” In vitro compartmentalization (IVC) is an in…”
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Macromolecular Complexation between Bovine Serum Albumin and the Self-Assembled Hydrogel Nanoparticle of Hydrophobized Polysaccharides
Published in Journal of the American Chemical Society (03-07-1996)“…Macromolecular complexation between bovine serum albumin (BSA) and self-assembled hydrogel nanoparticle formed by the self-aggregation of cholesterol-bearing…”
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Microscopic Structure and Thermoresponsiveness of a Hydrogel Nanoparticle by Self-Assembly of a Hydrophobized Polysaccharide
Published in Macromolecules (24-02-1997)“…Various cholesterol-bearing pullulans (CHPs) with different molecular weights of the parent pullulan and degrees of substitution (DS) of the cholesteryl moiety…”
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Supramolecular Assembly between Nanoparticles of Hydrophobized Polysaccharide and Soluble Protein Complexation between the Self-Aggregate of Cholesterol-Bearing Pullulan and .alpha.-Chymotrypsin
Published in Macromolecules (01-12-1994)“…Complexation between alpha -chymotrypsin (Chy) and the self-aggregate of cholesterol-bearing pullulan (CHP) was studied by size exclusion column…”
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Web-Structured Films of an Amphiphilic Polymer from Water in Oil Emulsion: Fabrication and Characterization
Published in Langmuir (08-02-2000)“…Fabrication of micro surface morphology of cell culture substrates is one of the current topics in biomaterials research. Recently we found that regular…”
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Preparation of self-organized micro-patterned Polymer films having Cell Adhesive ligands
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Effect of Liposome Size on Internal RNA Replication Coupled with Replicase Translation
Published in Chembiochem : a European journal of chemical biology (01-07-2016)“…Cell membranes inhibit the diffusion of intracellular materials, and compartment size can strongly affect the intracellular biochemical reactions. To assess…”
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Self-assembly of polymer amphiphiles: thermodynamics of complexation between bovine serum albumin and self-aggregate of cholesterol-bearing pullulan
Published in Colloids and surfaces. A, Physicochemical and engineering aspects (31-07-1996)“…The interaction between bovine serum albumin (BSA) and a hydrogel nanoparticle formed by the self-aggregation of cholesterol-bearing pullulan (CHP) was…”
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Journal Article Conference Proceeding -
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Honeycomb-patterned thin films of amphiphilic polymers as cell culture substrates
Published in Materials Science & Engineering C (01-12-1999)“…Recently, we have found honeycomb patterns with sub-micron line width that form during the non-equilibrium process of cast film formation. The…”
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Mesoscopic patterning of cell adhesive substrates as novel biofunctional interfaces
Published in Materials Science & Engineering C (30-12-1999)“…In this report, cell adhesion to honeycomb-patterned films is described with respect to the dimensions of the honeycomb structure. The honeycomb-patterned…”
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Self-Assembly of Hydrophobized Polysaccharide: Structure of Hydrogel Nanoparticle and Complexation with Organic Compounds
Published in Proceedings of the Japan Academy, Series B (1995)“…Self-assembly of hydrophobized polysaccharides in water was investigated by dynamic and static light scatterings, and by fluorescence and circular dichroism…”
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Synthesis of amphiphilic polysiloxanes and their properties for formation of nano-aggregates
Published in Colloid and polymer science (01-05-2009)“…In this study, we prepared amphiphilic polysiloxanes by introduction of hydrophobic and hydrophilic parts into a water-soluble poly(3-aminopropyl)siloxane…”
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Chemoenzymatic synthesis of amylose-grafted polyacetylenes
Published in Polymer bulletin (Berlin, Germany) (01-02-2008)“…Summary This paper reports chemoenzymatic synthesis of amylose-grafted polyacetylenes according to the following reaction manners. Polymerization of a N…”
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