Modulation of development, growth dynamics, wall crystallinity, and infection sites in white clover root hairs by membrane chitolipooligosaccharides from Rhizobium leguminosarum biovar trifolii

We used bright-field, time-lapse video, cross-polarized, phase-contrast, and fluorescence microscopies to examine the influence of isolated chitolipooligosaccharides (CLOSs) from wild-type Rhizobium leguminosarum bv. trifolii on development of white clover root hairs, and the role of these bioactive...

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Bibliographic Details
Published in:	Journal of Bacteriology Vol. 178; no. 12; pp. 3621 - 3627
Main Authors:	Dazzo, F B, Orgambide, G G, Philip-Hollingsworth, S, Hollingsworth, R I, Ninke, K O, Salzwedel, J L
Format:	Journal Article
Language:	English
Published:	United States American Society for Microbiology 01-06-1996
Subjects:	Bacteria Bacteriology cell differentiation cell membranes Cell Wall - chemistry cell walls crecimiento croissance diferenciacion celular differenciation cellulaire epiderme epidermis estimulo Fabaceae - microbiology Flowers & plants glicolipidos glycolipide glycolipids Glycolipids - physiology growth infeccion infection Lipopolysaccharides - chemistry Lipopolysaccharides - metabolism medicago sativa membranas celulares membrane cellulaire Membranes pared celular paroi cellulaire pelos radicales Plant growth Plants, Medicinal poil absorbant racine raices Rhizobium leguminosarum Rhizobium leguminosarum - physiology rhizobium trifolii root hairs roots Scientific imaging Space life sciences stimuli stimulus Symbiosis Trifolium repens
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Summary:	We used bright-field, time-lapse video, cross-polarized, phase-contrast, and fluorescence microscopies to examine the influence of isolated chitolipooligosaccharides (CLOSs) from wild-type Rhizobium leguminosarum bv. trifolii on development of white clover root hairs, and the role of these bioactive glycolipids in primary host infection. CLOS action caused a threefold increase in the differentiation of root epidermal cells into root hairs. At maturity, root hairs were significantly longer because of an extended period of active elongation without a change in the elongation rate itself. Time-series image analysis showed that the morphological basis of CLOS-induced root hair deformation is a redirection of tip growth displaced from the medial axis as previously predicted. Further studies showed several newly described infection-related root hair responses to CLOSs, including the localized disruption of the normal crystallinity in cell wall architecture and the induction of new infection sites. The application of CLOS also enabled a NodC- mutant of R. leguminosarum bv. trifolii to progress further in the infection process by inducing bright refractile spot modifications of the deformed root hair walls. However, CLOSs did not rescue the ability of the NodC- mutant to induce marked curlings or infection threads within root hairs. These results indicate that CLOS Nod factors elicit several host responses that modulate the growth dynamics and symbiont infectibility of white clover root hairs but that CLOSs alone are not sufficient to permit successful entry of the bacteria into root hairs during primary host infection in the Rhizobium-clover symbiosis.
Bibliography:	F61 F ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0021-9193 1098-5530 1067-8832
DOI:	10.1128/jb.178.12.3621-3627.1996