HPTLC Method for the Simultaneous Estimation of Emtricitabine and Tenofovir in Tablet Dosage Form

A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of emtricitabine and tenofovir simultaneously in combined dosage form. The stationary phase used was precoated silica gel 60F 254. The mobile phase used was a...

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Bibliographic Details
Published in:	Indian journal of pharmaceutical sciences Vol. 71; no. 1; pp. 95 - 97
Main Authors:	Joshi, Maithilee, Nikalje, A P, Shahed, M, Dehghan, M
Format:	Journal Article
Language:	English
Published:	India Medknow Publications and Media Pvt. Ltd 01-01-2009 Medknow Publications & Media Pvt. Ltd Medknow Publications
Subjects:	Chromatography Emtricitabine Methods Pharmaceutical chemistry Pharmaceuticals Pharmacology Properties Short Communication Tablets (Medicine) Tenofovir Thin layer chromatography India HPTLC analysis emtricitabine tenofovir
Online Access:	Get full text
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Summary:	A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of emtricitabine and tenofovir simultaneously in combined dosage form. The stationary phase used was precoated silica gel 60F 254. The mobile phase used was a mixture of chloroform: methanol (9:1 v/v). The detection of spots was carried out at 265 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 1000 ng with regression coefficient of 0.9995. The proposed method can be successfully used to determine the drug content of marketed tablet formulation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0250-474X 1998-3743
DOI:	10.4103/0250-474X.51951