Flow cytometry of human reticulocytes based on RNA fluorescence

A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting. Histograms of fluorescence values of mature red blood cells and reticulocytes obtained by flow cytometry of stained blood sample...

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Bibliographic Details
Published in:	Cytometry (New York, N.Y.) Vol. 1; no. 5; pp. 313 - 320
Main Authors:	Tanke, H. J., Nieuwenhuis, I. A. B., Koper, G. J. M., Slats, J. C. M., Ploem, J. S.
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-03-1981
Subjects:	Anemia - blood Blood Cell Count - methods Flow Cytometry Fluorescent Dyes fluorescent RNA staining Humans pyronin Y Pyronine Reticulocytes Reticulocytes - analysis RNA - blood Spectrometry, Fluorescence Staining and Labeling
Online Access:	Get full text
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Summary:	A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting. Histograms of fluorescence values of mature red blood cells and reticulocytes obtained by flow cytometry of stained blood samples, contain more information than offered by conventional methods for counting reticulocytes. Analysis of the fluorescence histograms provides new descriptive parameters of the reticulocyte population. The potential importance of this method in experimental and routine hematology is illustrated for a number of clinical cases.
Bibliography:	Presented at Automated Cytology VII, Asilomar, California, November 25–30, 1979. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0196-4763 1097-0320
DOI:	10.1002/cyto.990010503