Search Results - "Nierhaus, KH"

The weird and wonderful world of bacterial ribosome regulation by Wilson, Daniel N, Nierhaus, Knud H

“…In every organism, translation of the genetic information into functional proteins is performed on the ribosome. In Escherichia coli up to 40% of the cell's…”
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Ribosomal protection proteins and their mechanism of tetracycline resistance by CONNELL, Sean R, TRACZ, Dobryan M, NIERHAUS, Knud H, TAYLOR, Diane E

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Ribosomal proteins in the spotlight by Wilson, Daniel N, Nierhaus, Knud H

“…The assignment of specific ribosomal functions to individual ribosomal proteins is difficult due to the enormous cooperativity of the ribosome; however,…”
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Dissection of the Mechanism for the Stringent Factor RelA by Wendrich, Thomas M, Blaha, Gregor, Wilson, Daniel N, Marahiel, Mohamed A, Nierhaus, Knud H

“…During conditions of nutrient deprivation, ribosomes are blocked by uncharged tRNA at the A site. The stringent factor RelA binds to blocked ribosomes and…”
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Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process by Valle, Mikel, Sengupta, Jayati, Swami, Neil K., Grassucci, Robert A., Burkhardt, Nils, Nierhaus, Knud H., Agrawal, Rajendra K., Frank, Joachim

“…During the elongation cycle of protein biosynthesis, the specific amino acid coded for by the mRNA is delivered by a complex that is comprised of the cognate…”
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The E-site story: the importance of maintaining two tRNAs on the ribosome during protein synthesis by Wilson, D N, Nierhaus, K H

“…In the sixties James Watson suggested a twosite model for the ribosome comprising the P site for the peptidyl transfer RNA (tRNA) before peptide-bond formation…”
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Dissecting the Ribosomal Inhibition Mechanisms of Edeine and Pactamycin: The Universally Conserved Residues G693 and C795 Regulate P-Site RNA Binding by Dinos, George, Wilson, Daniel N, Teraoka, Yoshika, Szaflarski, Witold, Fucini, Paola, Kalpaxis, Dimitrios, Nierhaus, Knud H

“…The crystal structures of the universal translation-initiation inhibitors edeine and pactamycin bound to ribosomal 30S subunit have revealed that edeine…”
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New Features of the Ribosome and Ribosomal Inhibitors: Non-Enzymatic Recycling, Misreading and Back-Translocation by Szaflarski, Witold, Vesper, Oliver, Teraoka, Yoshika, Plitta, Beata, Wilson, Daniel N., Nierhaus, Knud H.

“…We describe the optimization of a poly(Phe) synthesis system, the conditions of which have been applied for efficient translation of heteropolymeric mRNAs…”
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Species-specific antibiotic-ribosome interactions: implications for drug development by Wilson, Daniel N, Harms, Jörg M, Nierhaus, Knud H, Schlünzen, Frank, Fucini, Paola

“…In the cell, the protein synthetic machinery is a highly complex apparatus that offers many potential sites for functional interference and therefore…”
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EF-G-Dependent GTPase on the Ribosome. Conformational Change and Fusidic Acid Inhibition by Seo, Hyuk-Soo, Abedin, Sameem, Kamp, Detlev, Wilson, Daniel N, Nierhaus, Knud H, Cooperman, Barry S

“…Protein synthesis studies increasingly focus on delineating the nature of conformational changes occurring as the ribosome exerts its catalytic functions…”
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Maintaining the Ribosomal Reading Frame: The Influence of the E Site during Translational Regulation of Release Factor 2 by Márquez, Viter, Wilson, Daniel N, Tate, Warren P, Triana-Alonso, Francisco, Nierhaus, Knud H

“…Maintenance of the translation reading frame is one of the most remarkable achievements of the ribosome while decoding the information of an mRNA. Loss of the…”
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Protein Expression in Liposomes by Oberholzer, Thomas, Nierhaus, Knud H., Luisi, Pier Luigi

“…Compartmentalization is one of the key steps in the evolution of cellular structures and, so far, only few attempts have been made to model this kind of…”
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The Ribosome through the Looking Glass by Wilson, Daniel N., Nierhaus, Knud H.

“…For almost 20 years crystallographers have sought to solve the structure of the ribosome, the largest and most complicated RNA–protein complex in the cell. All…”
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A map of protein-rRNA distribution in the 70 S Escherichia coli ribosome by Svergun, D I, Nierhaus, K H

“…Neutron scattering exploits the enormous scattering difference between protons and deuterons. A set of 42 x-ray and neutron solution scattering curves from…”
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Three-Dimensional Structures of Translating Ribosomes by Cryo-EM by Gilbert, Robert J.C., Fucini, Paola, Connell, Sean, Fuller, Stephen D., Nierhaus, Knud H., Robinson, Carol V., Dobson, Christopher M., Stuart, David I.

“…Cryo-electron microscopy and image reconstruction techniques have been used to obtain three-dimensional maps for E. coli ribosomes stalled following…”
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Mechanism of Tet(O)-mediated tetracycline resistance by Connell, Sean R., Trieber, Catharine A., Dinos, George P., Einfeldt, Edda, Taylor, Diane E., Nierhaus, Knud H.

“…Tet(O) is an elongation factor‐like protein which confers resistance to the protein synthesis inhibitor tetracycline by promoting the release of the drug from…”
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Cell-Free Protein Expression and Functional Assay in Nanowell Chip Format by Angenendt, Philipp, Nyarsik, Lajos, Szaflarski, Witold, Glökler, Jörn, Nierhaus, Knud H, Lehrach, Hans, Cahill, Dolores J, Lueking, Angelika

“…The expression and characterization of large protein libraries requires high-throughput tools for rapid and cost-effective expression and screening. A…”
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Detection and Selective Dissociation of Intact Ribosomes in a Mass Spectrometer by Rostom, Adam A., Fucini, Paola, Benjamin, Dennis R., Juenemann, Ralf, Nierhaus, Knud H., Hartl, F. Ulrich, Dobson, Christopher M., Robinson, Carol V.

“…Intact Escherichia coli ribosomes have been projected into the gas phase of a mass spectrometer by means of nanoflow electrospray techniques. Species with…”
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Heteronuclear NMR Investigations of Dynamic Regions of Intact Escherichia coli Ribosomes by Christodoulou, John, Larsson, Göran, Fucini, Paola, Connell, Sean R., Pertinhez, Thelma A., Hanson, Charlotte L., Redfield, Christina, Nierhaus, Knud H., Robinson, Carol V., Schleucher, Jürgen, Dobson, Christopher M., Doudna, Jennifer A.

“…15N-1H NMR spectroscopy has been used to probe the dynamic properties of uniformly15N labeled Escherichia coli ribosomes. Despite the high molecular weight of…”
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Visualization of tRNA Movements on the Escherichia coli 70S Ribosome during the Elongation Cycle by Agrawal, Rajendra K., Christian M. T. Spahn, Penczek, Pawel, Grassucci, Robert A., Nierhaus, Knud H., Frank, Joachim

“…Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-Å resolution. The positions of…”
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