Search Results - "Nienow, Alvin W"

Reactor engineering in large scale animal cell culture by Nienow, Alvin W

“…This article mainly addresses the issues associated with the engineering of large-scale free suspension culture in agitated bioreactors >10,000 L because they…”
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Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors by Rafiq, Qasim A., Coopman, Karen, Nienow, Alvin W., Hewitt, Christopher J.

“…Production of human mesenchymal stem cells for allogeneic cell therapies requires scalable, cost‐effective manufacturing processes. Microcarriers enable the…”
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Culture of human mesenchymal stem cells on microcarriers in a 5 l stirred-tank bioreactor by Rafiq, Qasim A, Brosnan, Kathryn M, Coopman, Karen, Nienow, Alvin W, Hewitt, Christopher J

“…For the first time, fully functional human mesenchymal stem cells (hMSCs) have been cultured at the litre-scale on microcarriers in a stirred-tank 5 l…”
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The translation of cell-based therapies: clinical landscape and manufacturing challenges by Heathman, Thomas RJ, Nienow, Alvin W, McCall, Mark J, Coopman, Karen, Kara, Bo, Hewitt, Christopher J

“…Cell-based therapies have the potential to make a large contribution toward currently unmet patient need and thus effective manufacture of these products is…”
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A potentially scalable method for the harvesting of hMSCs from microcarriers by Nienow, Alvin W., Rafiq, Qasim A., Coopman, Karen, Hewitt, Christopher J.

“…•A scalable method based on agitation theory for harvesting hMSCs from microcarriers.•Harvesting involves cell detachment from microcarriers followed by…”
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Catalytic Conversion of Sodium Lignosulfonate to Vanillin: Engineering Aspects. Part 1. Effects of Processing Conditions on Vanillin Yield and Selectivity by Pacek, Andrzej W, Ding, Ping, Garrett, Mark, Sheldrake, Gary, Nienow, Alvin W

“…The commercial production of vanillin from sodium lignosulfonate under highly alkaline conditions, catalyzed by Cu2+ at elevated temperature and pressures up…”
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Characterization of human mesenchymal stem cells from multiple donors and the implications for large scale bioprocess development by Heathman, Thomas R.J., Rafiq, Qasim A., Chan, Alexander K.C., Coopman, Karen, Nienow, Alvin W., Kara, Bo, Hewitt, Christopher J.

“…•We have characterized human mesenchymal stem cell lines from multiple donors.•Variation in key cell characteristics despite conformity to ISCT minimum…”
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Expansion of human mesenchymal stem cells on microcarriers by Hewitt, Christopher J, Lee, Ken, Nienow, Alvin W, Thomas, Robert J, Smith, Mark, Thomas, Colin R

“…The effects on human mesenchymal stem cell growth of choosing either of two spinner flask impeller geometries, two microcarrier concentrations and two cell…”
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Macro- and micromixing studies in an unbaffled vessel agitated by a Rushton turbine by Assirelli, Melissa, Bujalski, Waldemar, Eaglesham, Archie, Nienow, Alvin W.

“…Macromixing characteristics, power number and visual observation of the vortex behaviour and micromixing in an unbaffled tank agitated with a Rushton turbine…”
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Expansion of bone marrow‐derived human mesenchymal stem/stromal cells (hMSCs) using a two‐phase liquid/liquid system by Hanga, Mariana P, Murasiewicz, Halina, Pacek, Andrzej W, Nienow, Alvin W, Coopman, Karen, Hewitt, Christopher J

“…BACKGROUND Human mesenchymal stem/stromal cells (hMSCs) are at the forefront of regenerative medicine applications due to their relatively easy isolation and…”
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Shannon entropy for local and global description of mixing by Lagrangian particle tracking by Guida, Antonio, Nienow, Alvin W., Barigou, Mostafa

“…If 100 dice cannot be cast simultaneously, one single die can be cast 100 times. On the basis of this simple principle, the experimental technique of positron…”
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The Impact of Fluid Dynamic Stress in Stirred Bioreactors – The Scale of the Biological Entity: A Personal View by Nienow, Alvin W.

“…From the start of industrial biotechnology, there has been a perception that biological entities are damaged by stirring, so‐called ‘shear damage'. Often, it…”
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Using positron emission particle tracking (PEPT) to study the turbulent flow in a baffled vessel agitated by a Rushton turbine: Improving data treatment and validation by Chiti, Fabio, Bakalis, Serafim, Bujalski, Waldemar, Barigou, Mostafa, Eaglesham, Archie, Nienow, Alvin W.

“…► PEPT is validated against PIV and LDA literature data for the turbulent flow from a Rushton turbine. ► Improving software to process the raw particle…”
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Generation of Hydrogen Gas during the Catalytic Oxidation of Sodium Lignosulfonate to Vanillin: Initial Results by Ding, Ping, Garrett, Mark, Loe, Øystein, Nienow, Alvin W, Pacek, Andrzej W

“…The production of vanillin from sodium lignosulfonate under highly alkaline conditions, catalyzed by Cu2+ and at elevated temperature and pressures, has been…”
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A comparison of high cell density fed-batch fermentations involving both induced and non-induced recombinant Escherichia coli under well-mixed small-scale and simulated poorly mixed large-scale conditions by Hewitt, Christopher J., Onyeaka, Helen, Lewis, Gareth, Taylor, Ian W., Nienow, Alvin W.

“…In this work, multi‐parameter flow cytometric techniques, coupled with dual colour fluorescent staining, have been used to study the metabolic consequences of…”
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Stirring and Stirred-Tank Reactors by Nienow, Alvin W.

“…Stirred‐tank reactors are used throughout the process industry. They are not only applied in the chemical industry, but also in newer industrial sectors, such…”
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Studies supporting the use of mechanical mixing in large scale beer fermentations by Nienow, Alvin W, McLeod, Georgina, Hewitt, Christopher J

“…Brewing fermentations have traditionally been undertaken without the use of mechanical agitation, with mixing being provided only by the fluid motion induced…”
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Quantitative Measurements of the Critical Impeller Speed for Solid‐Liquid Suspensions by Ye, Guichuan, Nienow, Alvin W., Alberini, Federico

“…A quantitative methodology for particle suspension assessment is presented. A new parameter, fmov/tot, the ratio of the mean number of moving particles to the…”
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Scale-up agitation criteria for Trichoderma reesei fermentation by Hardy, Nicolas, Augier, Frédéric, Nienow, Alvin W., Béal, Catherine, Ben Chaabane, Fadhel

“…•Batch fermentations of the fungus T. reesei were conducted at bench and commercial scale.•At bench scale, 4 different stirrers were used at similar tip speeds…”
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Scale‐up of an intensified bioprocess for the expansion of bovine adipose‐derived stem cells (bASCs) in stirred tank bioreactors by Hanga, Mariana Petronela, Raga, Fritz Anthony, Moutsatsou, Panagiota, Hewitt, Christopher J., Nienow, Alvin W., Wall, Ivan

“…Cultivated meat is an emerging field, aiming to establish the production of animal tissue for human consumption in an in vitro environment, eliminating the…”
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