Search Results - "Nielsen, Michael L"

Proteome-wide Identification of Poly(ADP-Ribosyl)ation Targets in Different Genotoxic Stress Responses by Jungmichel, Stephanie, Rosenthal, Florian, Altmeyer, Matthias, Lukas, Jiri, Hottiger, Michael O, Nielsen, Michael L

“…Poly(ADP-ribos)ylation (PARylation) is a reversible posttranslational modification found in higher eukaryotes. However, little is known about PARylation…”
Get full text

An Advanced Strategy for Comprehensive Profiling of ADP-ribosylation Sites Using Mass Spectrometry-based Proteomics by Hendriks, Ivo A., Larsen, Sara C., Nielsen, Michael L.

“…We augmented our MS-based proteomics strategy for the purification and identification of ADP-ribosylated peptides, through side-by-side comparison of…”
Get full text

Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells by Larsen, Sara C, Sylvestersen, Kathrine B, Mund, Andreas, Lyon, David, Mullari, Meeli, Madsen, Maria V, Daniel, Jeremy A, Jensen, Lars J, Nielsen, Michael L

“…The posttranslational modification of proteins by arginine methylation is functionally important, yet the breadth of this modification is not well…”
Get more information

Site-specific characterization of endogenous SUMOylation across species and organs by Hendriks, Ivo A., Lyon, David, Su, Dan, Skotte, Niels H., Daniel, Jeremy A., Jensen, Lars J., Nielsen, Michael L.

“…Small ubiquitin-like modifiers (SUMOs) are post-translational modifications that play crucial roles in most cellular processes. While methods exist to study…”
Get full text

Lysine Acetylation Targets Protein Complexes and Co-Regulates Major Cellular Functions by Choudhary, Chunaram, Kumar, Chanchal, Gnad, Florian, Nielsen, Michael L, Rehman, Michael, Walther, Tobias C, Olsen, Jesper V, Mann, Matthias

“…Lysine acetylation is a reversible posttranslational modification of proteins and plays a key role in regulating gene expression. Technological limitations…”
Get full text

A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles by Wagner, Sebastian A., Beli, Petra, Weinert, Brian T., Nielsen, Michael L., Cox, Jürgen, Mann, Matthias, Choudhary, Chunaram

“…Post-translational modification of proteins by ubiquitin is a fundamentally important regulatory mechanism. However, proteome-wide analysis of endogenous…”
Get full text

Proteome-wide identification of the endogenous ADP-ribosylome of mammalian cells and tissue by Martello, Rita, Leutert, Mario, Jungmichel, Stephanie, Bilan, Vera, Larsen, Sara C., Young, Clifford, Hottiger, Michael O., Nielsen, Michael L.

“…Although protein ADP-ribosylation is involved in diverse biological processes, it has remained a challenge to identify ADP-ribose acceptor sites. Here, we…”
Get full text

Optimized Fast and Sensitive Acquisition Methods for Shotgun Proteomics on a Quadrupole Orbitrap Mass Spectrometer by Kelstrup, Christian D, Young, Clifford, Lavallee, Richard, Nielsen, Michael L, Olsen, Jesper V

“…Advances in proteomics are continually driven by the introduction of new mass spectrometric instrumentation with improved performances. The recently introduced…”
Get full text

Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification by Tessarz, Peter, Santos-Rosa, Helena, Robson, Sam C., Sylvestersen, Kathrine B., Nelson, Christopher J., Nielsen, Michael L., Kouzarides, Tony

“…A description of a new histone modification, methylation of glutamine, on histone H2A in yeast and human cells. All-in-one protein and RNA methylation…”
Get full text

Serine-linked PARP1 auto-modification controls PARP inhibitor response by Prokhorova, Evgeniia, Zobel, Florian, Smith, Rebecca, Zentout, Siham, Gibbs-Seymour, Ian, Schützenhofer, Kira, Peters, Alessandra, Groslambert, Joséphine, Zorzini, Valentina, Agnew, Thomas, Brognard, John, Nielsen, Michael L., Ahel, Dragana, Huet, Sébastien, Suskiewicz, Marcin J., Ahel, Ivan

“…Poly(ADP-ribose) polymerase 1 (PARP1) and PARP2 are recruited and activated by DNA damage, resulting in ADP-ribosylation at numerous sites, both within PARP1…”
Get full text

Mapping Physiological ADP-Ribosylation Using Activated Ion Electron Transfer Dissociation by Buch-Larsen, Sara C., Hendriks, Ivo A., Lodge, Jean M., Rykær, Martin, Furtwängler, Benjamin, Shishkova, Evgenia, Westphall, Michael S., Coon, Joshua J., Nielsen, Michael L.

“…ADP-ribosylation (ADPr) is a post-translational modification that plays pivotal roles in a wide range of cellular processes. Mass spectrometry (MS)-based…”
Get full text

The regulatory landscape of the human HPF1- and ARH3-dependent ADP-ribosylome by Hendriks, Ivo A., Buch-Larsen, Sara C., Prokhorova, Evgeniia, Elsborg, Jonas D., Rebak, Alexandra K.L.F.S., Zhu, Kang, Ahel, Dragana, Lukas, Claudia, Ahel, Ivan, Nielsen, Michael L.

“…Despite the involvement of Poly(ADP-ribose) polymerase-1 (PARP1) in many important biological pathways, the target residues of PARP1-mediated ADP-ribosylation…”
Get full text

Serine ADP-ribosylation in Drosophila provides insights into the evolution of reversible ADP-ribosylation signalling by Fontana, Pietro, Buch-Larsen, Sara C., Suyari, Osamu, Smith, Rebecca, Suskiewicz, Marcin J., Schützenhofer, Kira, Ariza, Antonio, Rack, Johannes Gregor Matthias, Nielsen, Michael L., Ahel, Ivan

“…In the mammalian DNA damage response, ADP-ribosylation signalling is of crucial importance to mark sites of DNA damage as well as recruit and regulate repairs…”
Get full text

Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast by de Godoy, Lyris M.F, Olsen, Jesper V, Cox, Jurgen, Nielsen, Michael L, Hubner, Nina C, Frohlich, Florian, Walther, Tobias C, Mann, Matthias

“…Mass spectrometry is a powerful technology for the analysis of large numbers of endogenous proteins. However, the analytical challenges associated with…”
Get full text

A Dual Pressure Linear Ion Trap Orbitrap Instrument with Very High Sequencing Speed by Olsen, Jesper V., Schwartz, Jae C., Griep-Raming, Jens, Nielsen, Michael L., Damoc, Eugen, Denisov, Eduard, Lange, Oliver, Remes, Philip, Taylor, Dennis, Splendore, Maurizio, Wouters, Eloy R., Senko, Michael, Makarov, Alexander, Mann, Matthias, Horning, Stevan

“…Since its introduction a few years ago, the linear ion trap Orbitrap (LTQ Orbitrap) instrument has become a powerful tool in proteomics research. For high…”
Get full text

Mass Spectrometric Analysis of Lysine Ubiquitylation Reveals Promiscuity at Site Level by Danielsen, Jannie M.R., Sylvestersen, Kathrine B., Bekker-Jensen, Simon, Szklarczyk, Damian, Poulsen, Jon W., Horn, Heiko, Jensen, Lars J., Mailand, Niels, Nielsen, Michael L.

“…The covalent attachment of ubiquitin to proteins regulates numerous processes in eukaryotic cells. Here we report the identification of 753 unique lysine…”
Get full text

Regulation of Rad52-dependent replication fork recovery through serine ADP-ribosylation of PolD3 by Richards, Frederick, Llorca-Cardenosa, Marta J., Langton, Jamie, Buch-Larsen, Sara C., Shamkhi, Noor F., Sharma, Abhishek Bharadwaj, Nielsen, Michael L., Lakin, Nicholas D.

“…Although Poly(ADP-ribose)-polymerases (PARPs) are key regulators of genome stability, how site-specific ADP-ribosylation regulates DNA repair is unclear. Here,…”
Get full text

Specificity and Commonality of the Phosphoinositide-Binding Proteome Analyzed by Quantitative Mass Spectrometry by Jungmichel, Stephanie, Sylvestersen, Kathrine B., Choudhary, Chunaram, Nguyen, Steve, Mann, Matthias, Nielsen, Michael L.

“…Phosphoinositides (PIPs) play key roles in signaling and disease. Using high-resolution quantitative mass spectrometry, we identified PIP-interacting proteins…”
Get full text

Proteomic Analysis of Arginine Methylation Sites in Human Cells Reveals Dynamic Regulation During Transcriptional Arrest by Sylvestersen, Kathrine B., Horn, Heiko, Jungmichel, Stephanie, Jensen, Lars J., Nielsen, Michael L.

“…The covalent attachment of methyl groups to the side-chain of arginine residues is known to play essential roles in regulation of transcription, protein…”
Get full text

Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation by Mooser, Clémence, Symeonidou, Ioanna-Eleni, Leimbacher, Pia-Amata, Ribeiro, Alison, Shorrocks, Ann-Marie K., Jungmichel, Stephanie, Larsen, Sara C., Knechtle, Katja, Jasrotia, Arti, Zurbriggen, Diana, Jeanrenaud, Alain, Leikauf, Colin, Fink, Daniel, Nielsen, Michael L., Blackford, Andrew N., Stucki, Manuel

“…Induction of DNA double-strand breaks (DSBs) in ribosomal DNA (rDNA) repeats is associated with ATM-dependent repression of ribosomal RNA synthesis and…”
Get full text