Search Results - "Nielsen, Alex T"
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Increased production of L-serine in Escherichia coli through Adaptive Laboratory Evolution
Published in Metabolic engineering (01-01-2017)“…L-serine is a promising building block biochemical with a high theoretical production yield from glucose. Toxicity of L-serine is however prohibitive for…”
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Predictable tuning of protein expression in bacteria
Published in Nature methods (01-03-2016)“…The free EMOPEC web tool enables precise tuning of bacterial protein expression on the basis of small changes to the Shine-Dalgarno sequence; oligos can be…”
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Broad-Host-Range ProUSER Vectors Enable Fast Characterization of Inducible Promoters and Optimization of p‑Coumaric Acid Production in Pseudomonas putida KT2440
Published in ACS synthetic biology (15-07-2016)“…Pseudomonas putida KT2440 has gained increasing interest as a host for the production of biochemicals. Because of the lack of a systematic characterization of…”
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A metabolic reconstruction of Lactobacillus reuteri JCM 1112 and analysis of its potential as a cell factory
Published in Microbial cell factories (29-10-2019)“…Lactobacillus reuteri is a heterofermentative Lactic Acid Bacterium (LAB) that is commonly used for food fermentations and probiotic purposes. Due to its…”
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CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae
Published in ACS synthetic biology (20-11-2015)“…Homologous recombination (HR) in Saccharomyces cerevisiae has been harnessed for both plasmid construction and chromosomal integration of foreign DNA. Still,…”
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Genome-Based Comparison of All Species of the Genus Moorella , and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica
Published in Frontiers in microbiology (17-01-2020)“…Fermentation of gases provides a promising opportunity for the production of biochemicals from renewable resources, which has resulted in a growing interest in…”
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The Vibrio cholerae Chitin Utilization Program
Published in Proceedings of the National Academy of Sciences - PNAS (24-02-2004)“…Chitin, an insoluble polymer of GlcNAc, is an abundant source of carbon, nitrogen, and energy for marine microorganisms. Microarray expression profiling and…”
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A bistable switch and anatomical site control Vibrio cholerae virulence gene expression in the intestine
Published in PLoS pathogens (01-09-2010)“…A fundamental, but unanswered question in host-pathogen interactions is the timing, localization and population distribution of virulence gene expression…”
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Characterization of a Feedback-Resistant Mevalonate Kinase from the Archaeon Methanosarcina mazei
Published in Applied and Environmental Microbiology (01-11-2011)“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Adaptive laboratory evolution of Bacillus subtilis to overcome toxicity of lignocellulosic hydrolysate derived from Distiller's dried grains with solubles (DDGS)
Published in Metabolic engineering communications (01-06-2023)“…Microbial tolerance to toxic compounds formed during biomass pretreatment is a significant challenge to produce bio-based products from lignocellulose cost…”
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Trash to treasure: production of biofuels and commodity chemicals via syngas fermenting microorganisms
Published in Current opinion in biotechnology (01-06-2014)“…Graphical abstract…”
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Combining genetic engineering and bioprocess concepts for improved phenylpropanoid production
Published in Biotechnology and bioengineering (01-03-2023)“…The group of natural aromatic compounds known as phenylpropanoids has diverse applications, but current methods of production which are largely based on…”
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Genome‐wide identification of tolerance mechanisms toward p‐coumaric acid in Pseudomonas putida
Published in Biotechnology and bioengineering (01-03-2018)“…The soil bacterium Pseudomonas putida KT2440 has gained increasing biotechnological interest due to its ability to tolerate different types of stress. Here,…”
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Genome editing of lactic acid bacteria: opportunities for food, feed, pharma and biotech
Published in FEMS microbiology letters (01-01-2019)“…Abstract This mini-review provides a perspective of traditional, emerging and future applications of lactic acid bacteria (LAB) and how genome editing tools…”
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Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress
Published in Scientific reports (08-12-2015)“…Generation of multiple genomic alterations is currently a time consuming process. Here, a method was established that enables highly efficient and simultaneous…”
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Identification of a Novel Group of Bacteria in Sludge from a Deteriorated Biological Phosphorus Removal Reactor
Published in Applied and Environmental Microbiology (01-03-1999)“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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17
A Capture-SELEX Strategy for Multiplexed Selection of RNA Aptamers Against Small Molecules
Published in Methods in molecular biology (Clifton, N.J.) (2018)“…In vitro selection of aptamers that recognize small organic molecules has proven difficult, in part due to the challenge of immobilizing small molecules on…”
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A clean in-frame knockout system for gene deletion in Acetobacterium woodii
Published in Journal of biotechnology (20-07-2022)“…Acetogenic bacteria produce acetate following the fixation of CO2 via the Wood-Ljungdahl pathway. As such, they represent excellent process organisms for the…”
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The Ssr protein (T1E_1405) from Pseudomonas putida DOT-T1E enables oligonucleotide-based recombineering in platform strain P. putida EM42
Published in Biotechnology journal (01-10-2016)“…Some strains of the soil bacterium Pseudomonas putida have become in recent years platforms of choice for hosting biotransformations of industrial interest…”
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Laboratory evolution reveals general and specific tolerance mechanisms for commodity chemicals
Published in Metabolic engineering (01-03-2023)“…Although strain tolerance to high product concentrations is a barrier to the economically viable biomanufacturing of industrial chemicals, chemical tolerance…”
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