Genomic characterization of Aeromonas spp. isolates from striped catfish with motile Aeromonas septicemia and human bloodstream infections in Vietnam
spp. are commonly found in the aquatic environment and have been responsible for motile septicemia (MAS) in striped catfish, resulting in significant economic loss. These organisms also cause a range of opportunistic infections in humans with compromised immune systems. Here, we conducted a genomic...
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| Published in: | Microbial genomics Vol. 10; no. 5 |
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| Main Authors: | , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
England
Microbiology Society
01-05-2024
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | spp. are commonly found in the aquatic environment and have been responsible for motile
septicemia (MAS) in striped catfish, resulting in significant economic loss. These organisms also cause a range of opportunistic infections in humans with compromised immune systems. Here, we conducted a genomic investigation of 87
isolates derived from diseased catfish, healthy catfish and environmental water in catfish farms affected by MAS outbreaks in eight provinces in Mekong Delta (years: 2012-2022), together with 25 isolates from humans with bloodstream infections (years: 2010-2020). Genomics-based typing method precisely delineated
species while traditional methods such as
PCR and MALDI-TOF were unable identify
was found to be more prevalent than
in both diseased catfish and human infections.
sequence type (ST) 656 followed by
ST251 were the predominant virulent species-lineages in diseased catfish (43.7 and 20.7 %, respectively), while diverse STs were found in humans with bloodstream infections. There was evidence of widespread transmission of ST656 and ST251 on striped catfish in the Mekong Delta region. ST656 and ST251 isolates carried a significantly higher number of acquired antimicrobial resistance (AMR) genes and virulence factors in comparison to other STs. They, however, exhibited several distinctions in key virulence factors (i.e. lack of type IV pili and enterotoxin
in
), AMR genes (i.e. presence of
carbapenemase in
), and accessory gene content. To uncover potential conserved proteins of
spp. for vaccine development, pangenome analysis has unveiled 2202 core genes between ST656 and ST251, of which 78 proteins were in either outer membrane or extracellular proteins. Our study represents one of the first genomic investigations of the species distribution, genetic landscape, and epidemiology of
in diseased catfish and human infections in Vietnam. The emergence of antimicrobial resistant and virulent
strains underscores the needs of enhanced genomic surveillance and strengthening vaccine research and development in preventing
diseases in catfish and humans, and the search for potential vaccine candidates could focus on
core genes encoded for membrane and secreted proteins. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work The author(s) declare that there are no conflicts of interest. Supplement: All supporting data, code and protocols have been provided within the article or through supplementary data files. Four supplementary tables are available with the online version of this article. |
| ISSN: | 2057-5858 2057-5858 |
| DOI: | 10.1099/mgen.0.001248 |