Search Results - "Nguyen, Tam T. T. N"

Investigating surrogate cerebrospinal fluid matrix compositions for use in quantitative LC-MS analysis of therapeutic antibodies in the cerebrospinal fluid by Fogh, Jens Rose, Jacobsen, Anne-Marie, Nguyen, Tam T. T. N., Rand, Kasper D., Olsen, Line Rørbæk

“…As quantitative analysis of biotherapeutics in cerebrospinal fluid (CSF) with LC-MS becomes increasingly widespread, there is a need for method developments…”
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Cellular pathways of recombinant adeno-associated virus production for gene therapy by Sha, Sha, Maloney, Andrew J., Katsikis, Georgios, Nguyen, Tam N.T., Neufeld, Caleb, Wolfrum, Jacqueline, Barone, Paul W., Springs, Stacy L., Manalis, Scott R., Sinskey, Anthony J., Braatz, Richard D.

“…Recombinant adeno-associated viruses (rAAVs) are among the most important vectors for in vivo gene therapies. With the rapid development of gene therapy,…”
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method for studies on interactions between a gold-based drug and plasma proteins based on capillary electrophoresis with inductively coupled plasma mass spectrometry detection by Nguyen, Tam T. T. N, Østergaard, Jesper, Gammelgaard, Bente

“…An analytical method based on capillary electrophoresis (CE) and inductively coupled plasma mass spectrometry (ICP-MS) detection was developed for studies on…”
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Expression, Purification and Characterization of GMZ2’.10C, a Complex Disulphide-Bonded Fusion Protein Vaccine Candidate against the Asexual and Sexual Life-Stages of the Malaria-Causing Plasmodium falciparum Parasite by Mistarz, Ulrik H., Singh, Susheel K., Nguyen, Tam T. T. N., Roeffen, Will, Yang, Fen, Lissau, Casper, Madsen, Søren M., Vrang, Astrid, Tiendrebeogo, Régis W., Kana, Ikhlaq H., Sauerwein, Robert W., Theisen, Michael, Rand, Kasper D.

“…Purpose Production and characterization of a chimeric fusion protein (GMZ2’.10C) which combines epitopes of key malaria parasite antigens: glutamate-rich…”
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Selective analysis of human serum albumin based on SEC-ICP-MS after labelling with iophenoxic acid by Dersch, Julie Maria, Nguyen, Tam T. T. N., Østergaard, Jesper, Stürup, Stefan, Gammelgaard, Bente

“…Human serum albumin (HSA) is the most abundant protein in the human plasma. HSA has several physiological roles in the human body, including storage and…”
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Circularized and solubility‐enhanced MSPs facilitate simple and high‐yield production of stable nanodiscs for studies of membrane proteins in solution by Johansen, Nicolai Tidemand, Tidemand, Frederik Grønbæk, Nguyen, Tam T. T. N., Rand, Kasper Dyrberg, Pedersen, Martin Cramer, Arleth, Lise

“…Recently, an enzymatic reaction was utilized to covalently link the N and C termini of membrane scaffold proteins to produce circularized nanodiscs that were…”
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Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses by Jönsson, Alexander, Svejdal, Rasmus R, Bøgelund, Nanna, Nguyen, Tam T. T. N, Flindt, Henrik, Kutter, Jörg P, Rand, Kasper D, Lafleur, Josiane P

“…To improve the sample handling, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical research, we here investigate…”
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Molecular architecture of the Jumonji C family histone demethylase KDM5B by Dorosz, Jerzy, Kristensen, Line Hyltoft, Aduri, Nanda G., Mirza, Osman, Lousen, Rikke, Bucciarelli, Saskia, Mehta, Ved, Sellés-Baiget, Selene, Solbak, Sara Marie Øie, Bach, Anders, Mesa, Pablo, Hernandez, Pablo Alcon, Montoya, Guillermo, Nguyen, Tam T. T. N., Rand, Kasper D., Boesen, Thomas, Gajhede, Michael

“…The full length human histone 3 lysine 4 demethylase KDM5B (PLU-1/Jarid1B) has been studied using Hydrogen/Deuterium exchange mass spectrometry, homology…”
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Metallomics in drug development: characterization of a liposomal cisplatin drug formulation in human plasma by CE–ICP–MS by Nguyen, Tam T. T. N., Østergaard, Jesper, Stürup, Stefan, Gammelgaard, Bente

“…A capillary electrophoresis inductively coupled plasma mass spectrometry method for separation of free cisplatin from liposome-encapsulated cisplatin and…”
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Characterization of a liposome-based formulation of oxaliplatin using capillary electrophoresis: Encapsulation and leakage by Franzen, Ulrik, Nguyen, Tam T.T.N., Vermehren, Charlotte, Gammelgaard, Bente, Østergaard, Jesper

“…A capillary electrophoresis-based method to characterize a PEGylated liposomal drug formulation of the anti-cancer agent oxaliplatin was developed…”
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Analytical methods for process and product characterization of recombinant adeno-associated virus-based gene therapies by Gimpel, Andreas L., Katsikis, Georgios, Sha, Sha, Maloney, Andrew John, Hong, Moo Sun, Nguyen, Tam N.T., Wolfrum, Jacqueline, Springs, Stacy L., Sinskey, Anthony J., Manalis, Scott R., Barone, Paul W., Braatz, Richard D.

“…The optimization of upstream and downstream processes for production of recombinant adeno-associated virus (rAAV) with consistent quality depends on the…”
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Multidose transient transfection of human embryonic kidney 293 cells modulates recombinant adeno-associated virus2/5 Rep protein expression and influences the enrichment fraction of filled capsids by Srinivasan, Prasanna, Canova, Christopher T, Sha, Sha, Nguyen, Tam N T, Joseph, John, Sangerman, Jose, Maloney, Andrew J, Katsikis, Georgios, Ou, Rui Wen, Hong, Moo Sun, Ng, Jaclyn, Yuan, Arella, Antov, Daniel, Song, Sally, Chen, Wenyu, Neufeld, Caleb, Wolfrum, Jacqueline M, Barone, Paul W, Sinskey, Anthony J, Springs, Stacy L, Braatz, Richard D

“…Recombinant adeno-associated virus (rAAV) is a commonly used in vivo gene therapy vector because of its nonpathogenicity, long-term transgene expression, broad…”
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Mechanistic model for production of recombinant adeno-associated virus via triple transfection of HEK293 cells by Nguyen, Tam N.T., Sha, Sha, Hong, Moo Sun, Maloney, Andrew J., Barone, Paul W., Neufeld, Caleb, Wolfrum, Jacqueline, Springs, Stacy L., Sinskey, Anthony J., Braatz, Richard D.

“…Manufacturing of recombinant adeno-associated virus (rAAV) viral vectors remains challenging, with low yields and low full:empty capsid ratios in the harvest…”
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Sodium dodecyl sulfate polyacrylamide gel electrophoresis for direct quantitation of protein adsorption by Lee, Man Chung Gilbert, Wu, Kinny Sheung Yang, Nguyen, Tam N.T., Sun, Bingyun

“…A simple method, sodium dodecyl sulfate polyacrylamide gel electrophoresis coupled with direct protein adsorption analysis (SDS–PAGE/DPA), is presented here…”
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A simple sheathless CE-MS interface with a sub-micrometer electrical contact fracture for sensitive analysis of peptide and protein samples by Nguyen, Tam T.T.N., Petersen, Nickolaj J., Rand, Kasper D.

“…Online coupling of capillary electrophoresis (CE) to electrospray ionization mass spectrometry (MS) has shown considerable potential, however, technical…”
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Determination of platinum drug release and liposome stability in human plasma by CE-ICP-MS by Nguyen, Tam T.T.N., Østergaard, Jesper, Stürup, Stefan, Gammelgaard, Bente

“…An in vitro method for simultaneous assessment of platinum release and liposome stability of liposomal formulations in human plasma is demonstrated. The…”
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Molecular Basis of the Mechanisms Controlling MASTL by Hermida, Dario, Mortuza, Gulnahar B., Pedersen, Anna-Kathrine, Pozdnyakova, Irina, Nguyen, Tam T.T.N., Maroto, Maria, Williamson, Michael, Ebersole, Tasja, Cazzamali, Giuseppe, Rand, Kasper, Olsen, Jesper V., Malumbres, Marcos, Montoya, Guillermo

“…MASTL is a unique kinase because of a non-conserved insertion of 550 residues within its activation loop. Using different constructs of MASTL, we show that the…”
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The extraordinary thermal stability of EstA from S. islandicus is independent of post translational modifications by Stiefler‐Jensen, Daniel, Schwarz‐Linnet, Troels, de Lichtenberg, Casper, Nguyen, Tam T. T. N., Rand, Kasper D., Huang, Li, She, Qunxin, Teilum, Kaare

“…Enzymes from thermophilic and hyper‐thermophilic organisms have an intrinsic high stability. Understanding the mechanisms behind their high stability will be…”
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Investigating the utility of minimized sample preparation and high-resolution mass spectrometry for quantification of monoclonal antibody drugs by Nguyen, Tam T.T.N., Mistarz, Ulrik H., Costa, Narciso, Herbet, Amaury, Boquet, Didier, Becher, François, Rand, Kasper D.

“…•Three different LC–MS/MS methods using QqQ, Q-TOF and Q-Orbitrap were developed and compared for quantitation of a model mAb in biological fluids.•The…”
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Investigation of a liposomal oxaliplatin drug formulation by capillary electrophoresis hyphenated to inductively coupled plasma mass spectrometry (CE-ICP-MS) by Nguyen, Tam T. T. N., Østergaard, Jesper, Stürup, Stefan, Gammelgaard, Bente

“…A capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS) method was developed for separation of the free oxaliplatin drug substance…”
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