The emergence of plasmid-borne cfr-mediated linezolid resistant-staphylococci in Vietnam

•Linezolid resistant coagulase-negative staphylococci (LRCNS) emerged in Vietnam.•The two LRCNS carry mutations in ribosome components, as well as transferrable linezolid resistance gene, cfr (chloramphenicol/florfenicol resistance).•The cfr gene locates on plasmids closely related to pLRSA417 previ...

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Published in:	Journal of global antimicrobial resistance. Vol. 22; pp. 462 - 465
Main Authors:	Nguyen, Le Thuy Thi, Nguyen, Khanh Ngau Thi, Le, Phuc Nhi Thi Anh, Cafini, Fabio, Pascoe, Ben, Sheppard, Samuel K., Nguyen, Thanh Bao, Nguyen, Thien Phuc Hoang, Nguyen, Thuy Vy, Pham, Tram T.K., Morikawa, Kazuya, Nguyen, Dang Quan, Duong, Hoa Xo
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Ltd 01-09-2020 Elsevier
Subjects:	cfr Coagulase-negative staphylococci Linezolid resistance Vietnam Linezolid resistance Vietnam cfr Coagulase-negative staphylococci
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Summary:	•Linezolid resistant coagulase-negative staphylococci (LRCNS) emerged in Vietnam.•The two LRCNS carry mutations in ribosome components, as well as transferrable linezolid resistance gene, cfr (chloramphenicol/florfenicol resistance).•The cfr gene locates on plasmids closely related to pLRSA417 previously found in China.•Dissemination of the cfr gene among staphylococcal species is anticipated. Linezolid is one of the last resort antibiotics effectively used in the treatment of infections caused by multidrug-resistant Gram-positive bacteria. Recent outbreaks of Linezolid resistance have been the great concern worldwide, while many countries have not experienced it. In this work, we aimed to evaluate the existence of linezolid resistance and further clarify potential resistance mechanism(s) in staphylococcal isolates obtained from the hospital in Vietnam, a country in which linezolid resistance had not been previously detected. Seventy staphylococcal clinical isolates including MRSA (n=63) and methicillin-resistant coagulase-negative staphylococci (MRCNS, n=7) were collected and analyzed for linezolid resistance. Linezolid-resistant isolates were submitted for whole genome sequencing to search for the resistance determinants. We identified two coagulase-negative staphylococcal isolates that were resistant to linezolid. Whole genome sequencing revealed several alterations in the 23S rRNA and L3, L17, L22, L24, L30 ribosomal proteins. Importantly, both isolates harbour the chloramphenicol/florfenicol resistance (cfr) gene on a plasmid. The plasmid was closely identical to the pLRSA417 plasmid that was originally reported in China. To the best of our knowledge, this is the first report of cfr-mediated linezolid resistance in clinically isolated staphylococci in Vietnam. We suggest that adequate surveillance is necessary to monitor the dissemination of linezolid resistance among staphylococcal species and other important pathogens.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2213-7165 2213-7173
DOI:	10.1016/j.jgar.2020.04.008