Search Results - "Nguyen, Giang K. T"

Butelase 1 is an Asx-specific ligase enabling peptide macrocyclization and synthesis by Nguyen, Giang K T, Wang, Shujing, Qiu, Yibo, Hemu, Xinya, Lian, Yilong, Tam, James P

“…Peptide macrocycles are attracting increasing attention as tools for research and as potential therapeutics. The highly efficient butelase 1—homologous to…”
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Total Synthesis of Circular Bacteriocins by Butelase 1 by Hemu, Xinya, Qiu, Yibo, Nguyen, Giang K. T, Tam, James P

“…Circular bacteriocins, ranging from 35 to 70 amino acids, are the largest cyclic peptides produced by lactic acid bacteria to suppress growth of other…”
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Characterization and application of natural and recombinant butelase-1 to improve industrial enzymes by end-to-end circularization by Hemu, Xinya, Zhang, Xiaohong, Nguyen, Giang K. T, To, Janet, Serra, Aida, Loo, Shining, Sze, Siu Kwan, Liu, Chuan-Fa, Tam, James P

“…Butelase-1, an asparaginyl endopeptidase or legumain, is the prototypical and fastest known Asn/Asp-specific peptide ligase. It is highly useful for…”
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Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation by Cao, Yuan, Nguyen, Giang K T, Tam, James P, Liu, Chuan-Fa

“…Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it…”
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Engineering a Catalytically Efficient Recombinant Protein Ligase by Yang, Renliang, Wong, Yee Hwa, Nguyen, Giang K T, Tam, James P, Lescar, Julien, Wu, Bin

“…Breaking and forming peptidyl bonds are fundamental biochemical reactions in protein chemistry. Unlike proteases that are abundantly available, fast-acting…”
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Butelase-mediated cyclization and ligation of peptides and proteins by Nguyen, Giang K T, Qiu, Yibo, Cao, Yuan, Hemu, Xinya, Liu, Chuan-Fa, Tam, James P

“…This protocol from Nguyen et al . describes the use of the plant cyclase butelase 1 for the efficient cyclization and ligation of peptides and proteins. After…”
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Butelase-Mediated Macrocyclization of d-Amino-Acid-Containing Peptides by Nguyen, Giang K. T., Hemu, Xinya, Quek, Jun-Ping, Tam, James P.

“…Macrocyclic compounds have received increasing attention in recent years. With their large surface area, they hold promise for inhibiting protein–protein…”
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Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity by Ngo, Khac Huy, Yang, Renliang, Das, Poulomi, Nguyen, Giang K T, Lim, Kah Wai, Tam, James P, Wu, Bin, Phan, Anh Tuân

“…G-quadruplexes (G4) are non-canonical nucleic acid structures with important implications in biology. Based on an α-helical fragment of the RHAU helicase that…”
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Butelase-Mediated Ligation as an Efficient Bioconjugation Method for the Synthesis of Peptide Dendrimers by Cao, Yuan, Nguyen, Giang K. T, Chuah, Samuel, Tam, James P, Liu, Chuan-Fa

“…Herein we report a novel enzymatic bioconjugation method to prepare peptide dendrimers. Under the catalysis of a newly discovered peptide ligase, butelase 1,…”
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Butelase 1: A Versatile Ligase for Peptide and Protein Macrocyclization by Nguyen, Giang K. T, Kam, Antony, Loo, Shining, Jansson, Anna E, Pan, Lucy X, Tam, James P

“…Macrocyclization is a valuable tool for drug design and protein engineering. Although various methods have been developed to prepare macrocycles, a general and…”
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Allotides: Proline-Rich Cystine Knot α‑Amylase Inhibitors from Allamanda cathartica by Nguyen, Phuong Q. T, Luu, Thuy T, Bai, Yang, Nguyen, Giang K. T, Pervushin, Konstantin, Tam, James P

“…Cystine knot α-amylase inhibitors belong to a knottin family of peptidyl inhibitors of 30–32 residues and contain two to four prolines. Thus far, only four…”
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Enzymatic Engineering of Live Bacterial Cell Surfaces Using Butelase 1 by Bi, Xiaobao, Yin, Juan, Nguyen, Giang K. T., Rao, Chang, Halim, Nurashikin Bte Abdul, Hemu, Xinya, Tam, James P., Liu, Chuan‐Fa

“…Butelase‐mediated ligation (BML) can be used to modify live bacterial cell surfaces with diverse cargo molecules. Surface‐displayed butelase recognition motif…”
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Site-Specific N-Terminal Labeling of Peptides and Proteins using Butelase 1 and Thiodepsipeptide by Nguyen, Giang K. T., Cao, Yuan, Wang, Wei, Liu, Chuan Fa, Tam, James P.

“…An efficient ligase with exquisite site‐specificity is highly desirable for protein modification. Recently, we discovered the fastest known ligase called…”
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Orally Active Peptidic Bradykinin B1 Receptor Antagonists Engineered from a Cyclotide Scaffold for Inflammatory Pain Treatment by Wong, Clarence T. T., Rowlands, Dewi K., Wong, Chi-Hang, Lo, Theodore W. C., Nguyen, Giang K. T., Li, Hoi-Yeung, Tam, James P.

“…Edible: By grafting natural peptide antagonists onto the cyclotide kalata B1, orally active peptides were engineered, which are potentially useful therapeutics…”
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Ginsentides: Cysteine and Glycine-rich Peptides from the Ginseng Family with Unusual Disulfide Connectivity by Tam, James P., Nguyen, Giang K. T., Loo, Shining, Wang, Shujing, Yang, Daiwen, Kam, Antony

“…Ginseng, a popular and valuable traditional medicine, has been used for centuries to maintain health and treat disease. Here we report the discovery and…”
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Butelase-Mediated Macrocyclization of d-Amino-Acid-Containing Peptides by Nguyen, Giang K. T., Hemu, Xinya, Quek, Jun-Ping, Tam, James P.

“…Macrocyclic compounds have received increasing attention in recent years. With their large surface area, they hold promise for inhibiting protein–protein…”
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Identification and Characterization of Roseltide, a Knottin-type Neutrophil Elastase Inhibitor Derived from Hibiscus sabdariffa by Loo, Shining, Kam, Antony, Xiao, Tianshu, Nguyen, Giang K. T., Liu, Chuan Fa, Tam, James P.

“…Plant knottins are of therapeutic interest due to their high metabolic stability and inhibitory activity against proteinases involved in human diseases. The…”
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A high-throughput peptidomic strategy to decipher the molecular diversity of cyclic cysteine-rich peptides by Serra, Aida, Hemu, Xinya, Nguyen, Giang K. T., Nguyen, Ngan T. K., Sze, Siu Kwan, Tam, James P.

“…Cyclotides are plant cyclic cysteine-rich peptides (CRPs). The cyclic nature is reported to be gene-determined with a precursor containing a…”
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Site-Specific N-Terminal Labeling of Peptides and Proteins using Butelase 1 and Thiodepsipeptide by Nguyen, Giang K. T., Cao, Yuan, Wang, Wei, Liu, Chuan Fa, Tam, James P.

“…An efficient ligase with exquisite site‐specificity is highly desirable for protein modification. Recently, we discovered the fastest known ligase called…”
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Site-Specific N-Terminal Labeling of Peptides and Proteins using Butelase1 and Thiodepsipeptide by Nguyen, Giang K T, Cao, Yuan, Wang, Wei, Liu, Chuan Fa, Tam, James P

“…An efficient ligase with exquisite site-specificity is highly desirable for protein modification. Recently, we discovered the fastest known ligase called…”
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