Comparison of pancreatic enzyme abnormalities and protease-activated receptor-2-positive eosinophils in the duodenum of patients with functional dyspepsia-irritable bowel syndrome overlap with functional dyspepsia alone in Asian populations

Some patients with functional gastrointestinal disorders exhibit pancreatic dysfunctions and pancreatic enzyme abnormalities. Thus, we aimed to clarify whether significant differences in clinical characteristics, prevalence of pancreatic enzyme abnormalities, duodenal inflammation, and protease-acti...

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Published in:	Journal of gastroenterology and hepatology Vol. 38; no. 10; pp. 1778 - 1786
Main Authors:	Futagami, Seiji, Kessoku, Takaomi, Kasai, Yuki, Higurashi, Takuma, Nakajima, Atsushi, Agawa, Shuhei, Yamawaki, Hiroshi, Nakamura, Ken, Habiro, Mayu, Kawawa, Rie, Ueki, Nobue, Higashida, Sakura, Watanabe, Yoshiyuki, Yamato, Hiroshi, Yamamoto, Takatsugu, Takasaki, Yusuke, Ito, Koichi, Hojo, Mariko, Isayama, Hiroyuki, Motoda, Norio, Ohashi, Ryuji, Siah, Kewin Tien Ho, Ng, Carissa Kahmun, Gwee, Kok-Ann
Format:	Journal Article
Language:	English
Published:	Australia Wiley Subscription Services, Inc 01-10-2023
Subjects:	Duodenum Dyspepsia Elastase Enzymes Eotaxin Gastrointestinal diseases Hypersensitivity Intestine Irritable bowel syndrome Leukocytes (eosinophilic) mRNA Pancreas Phospholipase A2 Small intestine Trypsin protease activated receptor 2 (PAR2) degranulated eosinophil irritable bowel syndrome (IBS) functional dyspepsia (FD) FD-IBS overlap
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Summary:	Some patients with functional gastrointestinal disorders exhibit pancreatic dysfunctions and pancreatic enzyme abnormalities. Thus, we aimed to clarify whether significant differences in clinical characteristics, prevalence of pancreatic enzyme abnormalities, duodenal inflammation, and protease-activated receptor 2 (PAR2) expression levels related to hypersensitivity exist between functional dyspepsia (FD) alone and FD-irritable bowel syndrome (IBS) overlap group. Ninety-three patients based on the Rome IV criteria, FD alone (n = 44) and FD overlapped with IBS (n = 49) group were enrolled. The patients scored their own clinical symptoms after consuming high-fat meals. Serum trypsin, PLA2, lipase, p-amylase, and elastase-1 levels were measured. PAR2, eotaxin-3, and TRPV4 mRNA levels in duodenum were determined using real-time polymerase chain reaction methods. PRG2- and PAR2 in the duodenum were evaluated using immunostaining. FD score and global GSRS in patients with FD-IBS overlap were significantly higher than FD alone. Although the prevalence of pancreatic enzyme abnormalities in patients with FD alone was significantly (P < 0.01) higher than that in FD-IBS overlap, the ratio of aggravation of clinical symptoms following high-fat intake in patients with FD-IBS overlap was significantly higher (P = 0.007) than that in patients with FD alone. PAR2- and PRG2-double positive cells were localized in the degranulated eosinophils in the duodenum of patients with FD-IBS overlap. The number of PAR2- and PRG2-double positive cells in FD-IBS overlap was significantly (P < 0.01) higher than FD alone. Pancreatic enzyme abnormalities and PAR2 expression on degranulated eosinophils infiltrations in the duodenum may be associated with the pathophysiology of patients with FD-IBS overlap in Asian populations.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0815-9319 1440-1746
DOI:	10.1111/jgh.16250