Search Results - "Newmyer, S L"

Horseradish peroxidase-catalyzed two-electron oxidations. Oxidation of iodide, thioanisoles, and phenols at distinct sites by HARRIS, R. Z, NEWMYER, S. L, ORTIZ DE MONTELLANO, P. R

“…The atypical two-electron oxidation of thioanisole and its p-methyl, p-methoxy, and p-nitro analogues by horseradish peroxidase, contrary to earlier reports,…”
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Dominant-Interfering Hsc70 Mutants Disrupt Multiple Stages of the Clathrin-Coated Vesicle Cycle in vivo by Newmyer, Sherri L., Schmid, Sandra L.

“…Within the clathrin-coated vesicle (CCV) cycle, coat assembly drives the internalization of receptors from the cell surface and disassembly allows for the…”
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Hsc70 Is Required for Endocytosis and Clathrin Function in Drosophila by Chang, Henry C., Newmyer, Sherri L., Hull, Michael J., Ebersold, Melanie, Schmid, Sandra L., Mellman, Ira

“…By screening for Drosophila mutants exhibiting aberrant bride of sevenless (Boss) staining patterns on eye imaginal disc epithelia, we have recovered a point…”
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Auxilin-Dynamin Interactions Link the Uncoating ATPase Chaperone Machinery with Vesicle Formation by Newmyer, Sherri L, Christensen, Arne, Sever, Sanja

“…The large GTPase dynamin is required for budding of clathrin-coated vesicles from the plasma membrane, after which the clathrin coat is removed by the…”
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Horseradish peroxidase His-42 --> Ala, His-42 --> Val, and Phe-41 --> Ala mutants. Histidine catalysis and control of substrate access to the heme iron by Newmyer, S L, Ortiz de Montellano, P R

“…Polyhistidine-tagged horseradish peroxidase (hHRP) and its F41A, H42A, and H42V mutants have expressed in an insect cell system. Kinetic studies show that the…”
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Structural determinants of the catalytic activities of peroxidases by Ortiz de Montellano, P R, Ozaki, S I, Newmyer, S L, Miller, V P, Harmann, C

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Uncoating ATPase Hsc70 is Recruited by Invariant Chain and Controls the Size of Endocytic Compartments by Lagaudriére-Gesbert, Cécile, Newmyer, Sherri L., Gregers, Tone F., Bakke, Oddmund, Ploegh, Hidde L.

“…Targeting of class II major histocompatibility complex molecules to endocytic compartments is mediated by their association with the invariant chain (li)…”
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ATP- and cytosol-dependent release of adaptor proteins from clathrin-coated vesicles: A dual role for Hsc70 by Hannan, L A, Newmyer, S L, Schmid, S L

“…Clathrin-coated vesicles (CCV) mediate protein sorting and vesicular trafficking from the plasma membrane and the trans-Golgi network. Before delivery of the…”
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Rescue of the Catalytic Activity of an H42A Mutant of Horseradish Peroxidase by Exogenous Imidazoles by Newmyer, S L, de Montellano, P R

“…His-42 plays a critical role in the H 2 O 2 -dependent catalytic turnover of horseradish peroxidase (HRP). This is clearly illustrated by the finding that an…”
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Rescue of His-42 --> Ala horseradish peroxidase by a Phe-41 --> His mutation. Engineering of a surrogate catalytic histidine by Savenkova, M I, Newmyer, S L, Montellano, P R

“…Formation of the ferryl (FeIV=O) porphyrin radical cation known as Compound I in the reaction of horseradish peroxidase (HRP) with H2O2 is catalyzed by His-42,…”
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Rescue of the Horseradish Peroxidase His-170 → Ala Mutant Activity by Imidazole:  Importance of Proximal Ligand Tethering by Newmyer, Sherri L, Sun, Jie, Loehr, Thomas M, Ortiz de Montellano, Paul R

“…The proximal iron ligand in horseradish peroxidase (HRP) is His-170. The H170A mutant of polyhistidine-tagged HRP (hHRP) has been expressed in a baculovirus…”
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Labeling antibodies with copper radionuclides using N-4-nitrobenzyl-5-(4-carboxyphenyl)-10,15,20-tris(4-sulfophenyl) porphine by Roberts, J C, Newmyer, S L, Mercer-Smith, J A, Schreyer, S A, Lavallee, D K

“…Antibody conjugates labeled with copper-64 and -67 (64Cu and 67Cu) were prepared using the porphyrin chelator…”
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Horseradish peroxidase His-42 arrow right Ala, His-42 arrow right Val, and Phe-41 arrow right Ala mutants: Histidine catalysis and control of substrate access to the heme iron by Newmyer, S L, De Montellano, PRO

“…Polyhistidine-tagged horseradish peroxidase (hHRP) and its F41A, H42A, and H42V mutants have been expressed in an insect cell system. Kinetic studies show that…”
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Solution 1H NMR of the Molecular and Electronic Structure of the Heme Cavity and Substrate Binding Pocket of High-Spin Ferric Horseradish Peroxidase:  Effect of His42Ala Mutation by Asokan, Anbanandam, de Ropp, Jeffrey S, Newmyer, Sherri L, Ortiz de Montellano, Paul R, La Mar, Gerd N

“…Solution 1H NMR has been used to assign a major portion of the heme environment and the substrate-binding pocket of resting state horseradish peroxidase, HRP,…”
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Rescue of His-42 arrow right Ala horseradish peroxidase by a Phe-41 arrow right His mutation: Engineering of a surrogate catalytic histidine by Savenkova, MI, Newmyer, S L, Ortiz de Montellano, PR

“…Formation of the ferryl (Fe super(IV)=O) porphyrin radical cation known as Compound I in the reaction of horseradish peroxidase (HRP) with H sub(2)O sub(2) is…”
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Assays and functional properties of auxilin-dynamin interactions by Sever, Sanja, Skoch, Jesse, Bacskai, Brian J, Newmyer, Sherri L

“…The large GTPase dynamin is required for budding of clathrin-coated vesicles from the plasma membrane, but its mechanism of action is still not understood…”
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