Selective optogenetic inhibition of Gα q or Gα i signaling by minimal RGS domains disrupts circuit functionality and circuit formation

Optogenetic techniques provide genetically targeted, spatially and temporally precise approaches to correlate cellular activities and physiological outcomes. In the nervous system, G protein-coupled receptors (GPCRs) have essential neuromodulatory functions through binding extracellular ligands to i...

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Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 121; no. 36; p. e2411846121
Main Authors:	Lockyer, Jayde L, Reading, Andrew, Vicenzi, Silvia, Zbela, Agnieszka, Viswanathan, Saranya, Delandre, Caroline, Newland, Jake W, McMullen, John P D, Marshall, Owen J, Gasperini, Robert, Foa, Lisa, Lin, John Y
Format:	Journal Article
Language:	English
Published:	United States National Academy of Sciences 03-09-2024
Subjects:	Animals Axon guidance Caenorhabditis elegans - metabolism Developmental stages Dorsal root ganglia Drosophila - metabolism Embryos G protein-coupled receptors Ganglia Ganglia, Spinal - cytology Ganglia, Spinal - metabolism GTP-Binding Protein alpha Subunits, Gi-Go - genetics GTP-Binding Protein alpha Subunits, Gi-Go - metabolism GTP-Binding Protein alpha Subunits, Gq-G11 - genetics GTP-Binding Protein alpha Subunits, Gq-G11 - metabolism Intracellular signalling Larvae Nervous system Neuromodulation Neurons - metabolism Optogenetics - methods Protein Domains Proteins Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - metabolism RGS Proteins - genetics RGS Proteins - metabolism Serotonin Signal Transduction Zebrafish Zebrafish - embryology GPCR trimeric G protein optogenetics RGS
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Summary:	Optogenetic techniques provide genetically targeted, spatially and temporally precise approaches to correlate cellular activities and physiological outcomes. In the nervous system, G protein-coupled receptors (GPCRs) have essential neuromodulatory functions through binding extracellular ligands to induce intracellular signaling cascades. In this work, we develop and validate an optogenetic tool that disrupts Gα signaling through membrane recruitment of a minimal regulator of G protein signaling (RGS) domain. This approach, Photo-induced Gα Modulator-Inhibition of Gα (PiGM-Iq), exhibited potent and selective inhibition of Gα signaling. Using PiGM-Iq we alter the behavior of and with outcomes consistent with GPCR-Gα disruption. PiGM-Iq changes axon guidance in cultured dorsal root ganglia neurons in response to serotonin. PiGM-Iq activation leads to developmental deficits in zebrafish embryos and larvae resulting in altered neuronal wiring and behavior. Furthermore, by altering the minimal RGS domain, we show that this approach is amenable to Gα signaling. Our unique and robust optogenetic Gα inhibiting approaches complement existing neurobiological tools and can be used to investigate the functional effects neuromodulators that signal through GPCR and trimeric G proteins.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0027-8424 1091-6490 1091-6490
DOI:	10.1073/pnas.2411846121