A comparison of dopaminergic cells from the human NTera2/D1 cell line transplanted into the hemiparkinsonian rat

Human NT cells derived from the NTera2/D1 cell line express a dopaminergic phenotype making them an attractive vehicle to supply dopamine to the depleted striatum of the Parkinsonian patient. In vitro, hNT neurons express tyrosine hydroxylase (TH), depending on the length of time they are exposed to...

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Bibliographic Details
Published in:	Life sciences (1973) Vol. 81; no. 6; pp. 441 - 448
Main Authors:	Newcomb, J.D., Janowski, M., Zigova, T., Saporta, S., McGrogan, M.P., Sanberg, P.R., Willing, A.E.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Inc 19-07-2007
Subjects:	Animals Apomorphine Apomorphine - toxicity Behavior, Animal - drug effects Cell Line Cell Transplantation - physiology Dopamine - physiology Dopamine Agonists - toxicity Graft Survival hNT Humans Image Processing, Computer-Assisted Immunohistochemistry Oxidopamine Parkinson Disease, Secondary - chemically induced Parkinson Disease, Secondary - physiopathology Parkinson's Disease Rats Rats, Sprague-Dawley Receptors, Dopamine D1 - genetics Receptors, Dopamine D1 - physiology Retinoic acid Stereotyped Behavior - drug effects Sympatholytics Transplantation Tyrosine 3-Monooxygenase - biosynthesis Tyrosine 3-Monooxygenase - genetics Transplantation Parkinson's Disease Retinoic acid Apomorphine hNT
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Summary:	Human NT cells derived from the NTera2/D1 cell line express a dopaminergic phenotype making them an attractive vehicle to supply dopamine to the depleted striatum of the Parkinsonian patient. In vitro, hNT neurons express tyrosine hydroxylase (TH), depending on the length of time they are exposed to retinoic acid. This study compared two populations of hNT neurons that exhibit a high yield of TH+ cells, MI-hNT and DA-hNT. The MI-hNT and DA-hNT neurons were intrastriatally transplanted into the 6-OHDA hemiparkinsonian rat. Amelioration in rotational behavior was measured and immunohistochemistry was performed to identify surviving hNT and TH+ hNT neurons. Results indicated that both MI-hNT and DA-hNT neurons can survive in the striatum, however, neither maintained their dopaminergic phenotype in vivo. Other strategies used in conjunction with hNT cell replacement are likely needed to enhance and maintain the dopamine expression in the grafted cells.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0024-3205 1879-0631
DOI:	10.1016/j.lfs.2007.05.025