Insecticidal proteins from Bacillus thuringiensis protect corn from corn rootworms

Field tests of corn co-expressing two new delta-endotoxins from Bacillus thuringiensis (Bt) have demonstrated protection from root damage by western corn rootworm (Diabrotica virgifera virgifera LeConte). The level of protection exceeds that provided by chemical insecticides. In the bacterium, these...

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Bibliographic Details
Published in:	Nature biotechnology Vol. 19; no. 7; pp. 668 - 672
Main Authors:	Moellenbeck, Daniel J, Peters, Melvin L, Bing, James W, Rouse, James R, Higgins, Laura S, Sims, Lynne, Nevshemal, Tony, Marshall, Lisa, Ellis, R. Tracy, Bystrak, Paul G, Lang, Bruce A, Stewart, James L, Kouba, Kristen, Sondag, Valerie, Gustafson, Vicki, Nour, Katy, Xu, Deping, Swenson, Jan, Zhang, Jian, Czapla, Thomas, Schwab, George, Jayne, Susan, Stockhoff, Brian A, Narva, Kenneth, Schnepf, H. Ernest, Stelman, Steven J, Poutre, Candace, Koziel, Michael, Duck, Nicholas
Format:	Journal Article
Language:	English
Published:	New York, NY Nature 01-07-2001 Nature Publishing Group
Subjects:	Agricultural production Animals Bacillus thuringiensis Bacillus thuringiensis - chemistry Bacterial Proteins - pharmacology Bacterial Toxins Biological and medical sciences Biological control Biotechnology Chrysomelidae Control Crop rotation crystal proteins Crystals d-Endotoxin Diabrotica virgifera Electrophoresis, Polyacrylamide Gel Endotoxins Endotoxins - pharmacology Field tests Fundamental and applied biological sciences. Psychology Hemolysin Proteins Immunity, Innate Immunoblotting Insect Control - methods Insecticides Larva Larvae Models, Genetic Pesticides Phytopathology. Animal pests. Plant and forest protection Plants, Genetically Modified Polypeptides Proteins Protozoa. Invertebrates Publishing Roots Soybeans Toxicity Toxins Transformation, Genetic Vegetables Zea mays Zea mays - metabolism United States > US Monocotyledones Chrysomelidae Coleoptera Insecta Bacillus thuringiensis Biological control Bacillaceae Field experiment Cereal crop Diabrotica virgifera virgifera Pest Bacillales Gramineae Angiospermae Bacteria Transgenic plant Biopesticide Zea mays Gene coexpression Gene expression Toxin Arthropoda Pest resistance Spermatophyta Parasporal body Invertebrata Microbial insecticide
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Summary:	Field tests of corn co-expressing two new delta-endotoxins from Bacillus thuringiensis (Bt) have demonstrated protection from root damage by western corn rootworm (Diabrotica virgifera virgifera LeConte). The level of protection exceeds that provided by chemical insecticides. In the bacterium, these proteins form crystals during the sporulation phase of the growth cycle, are encoded by a single operon, and have molecular masses of 14 kDa and 44 kDa. Corn rootworm larvae fed on corn roots expressing the proteins showed histopathological symptoms in the midgut epithelium.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1087-0156 1546-1696
DOI:	10.1038/90282