Study of biocatalyst to produce ethanol from starch: coimmobilization of glucoamylase and yeast in gel

This article presents a detailed study on the conditions for achieving a stable biocatalyst to be used in the production of ethanol from starch. Different pellets were used depending on which characteristic of the biocatalyst was being studied: (a) Saccharomyces cerevisiae entrapped in pectin or cal...

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Bibliographic Details
Published in:	Applied biochemistry and biotechnology Vol. 84; no. 1-9; pp. 643 - 654
Main Authors:	Giordano, R.L.C, Hirano, P.C, Goncalves, L.R.B, Netto, W.S
Format:	Journal Article Conference Proceeding
Language:	English
Published:	Heidelberg Springer 2000 Springer Nature B.V
Subjects:	acetates Alginates biocatalysts Biological and medical sciences Biotechnology calcium alginate calcium chloride Carbohydrates cassava Catalysis Catalysts Cells, Immobilized - metabolism Enzymes, Immobilized - metabolism Ethanol ethanol production Fermentation flour Fundamental and applied biological sciences. Psychology gels glucan 1,4-alpha-glucosidase Glucan 1,4-alpha-Glucosidase - metabolism Glucuronic Acid Hexuronic Acids hydrolysis Kinetics mechanical properties Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Pectins pellets Saccharomyces cerevisiae Saccharomyces cerevisiae - metabolism Silica Starch Starch - metabolism syrups viability Yeasts Glucan 1,4-α-glucosidase Yeast Ethanol Support Enzyme Starch Coimmobilization Pectin Fungi Glycosidases Production Microorganism culture Hydrolases Ascomycetes Cassava meal Saccharomyces cerevisiae Thallophyta
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Summary:	This article presents a detailed study on the conditions for achieving a stable biocatalyst to be used in the production of ethanol from starch. Different pellets were used depending on which characteristic of the biocatalyst was being studied: (a) Saccharomyces cerevisiae entrapped in pectin or calcium alginate gel particles; (b) silica containing immobilized glucoamylase entrapped in pectin gel particles; or (c) pectin gel particles, with the silica-enzyme derivative and yeast coimmobilized. The influence of several variables on the mechanical resistance of the particle, on the viability of the microorganism, and on the rate of substrate hydrolysis was studied with biocatalyst. The best conditions found were 6% pectin gel, 2-mm particle diameter, and cure in 0.2M CaCl2(.)2H2O/60 mM acetate buffer, pH 4.2, for gel preparation; and 6.0 g/L of CaCl2(.)2H2O in the fermentation medium. Biocatalyst (c) was successfully tested for the production of ethanol from liquefied manioc flour syrup.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0273-2289 1559-0291
DOI:	10.1385/ABAB:84-86:1-9:643