Use of photodynamic therapy and photobiomodulation as alternatives for microbial control on clinical and subclinical mastitis in sheep

Use of photodynamic therapy and photobiomodulation as alternatives for microbial control on clinical and subclinical mastitis in sheep

Evaluate the effects of antimicrobial photodynamic therapy (aPDT) and photobiomodulation (PBM) as alternatives in the treatment of mastitis in sheep. A total of 100 sheep were evaluated, and four teats with clinical mastitis and 16 teats with subclinical mastitis were selected. Milk was collected fo...

Full description

Saved in:
Bibliographic Details
Published in:Lasers in medical science Vol. 37; no. 4; pp. 2305 - 2310
Main Authors: Silva, Lara Oliveira, da Silva Souza, Kedma Lorena, de Jesus Beloti, Larissa, Neto, Waldemar Mota Ramos, Núñez, Silvia Cristina, Frias, Danila Fernanda Rodrigues
Format: Journal Article
Language:English
Published: London Springer London 01-06-2022
Springer Nature B.V
Subjects:
Antiinfectives and antibacterials
Biochemical tests
Coagulase
Dentistry
E coli
Escherichia coli
Lasers
Light therapy
Mastitis
Medicine
Medicine & Public Health
Methylene blue
Microorganisms
Optical Devices
Optics
Original Article
Photodynamic therapy
Photonics
Quantum Optics
Semiconductor lasers
Sheep
Staphylococcus
Streptococcus
Teats
Low-level laser therapy
Photoinactivation
aPDT
Photosensitizer
Small ruminants
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Evaluate the effects of antimicrobial photodynamic therapy (aPDT) and photobiomodulation (PBM) as alternatives in the treatment of mastitis in sheep. A total of 100 sheep were evaluated, and four teats with clinical mastitis and 16 teats with subclinical mastitis were selected. Milk was collected for isolation and identification of microorganisms. They were grown on TSA, EMB, and MacConkey agar for 24 h, and the microorganisms were identified by Gram stain and biochemical tests. The ceilings were subdivided into four groups: G1, treatment with photosensitizer; G2, treatment with PBM (diode laser λ  = 660 nm); G3, aPDT with methylene blue, and G4, control group. Milk samples were collected before, 24 and 48 h after treatments. Cases of subclinical mastitis presented coagulase-negative Staphylococcus and Streptococcus spp, and clinical mastitis had Escherichia coli grow from the samples. The treatments decrease the total bacterial count of negative coagulase Staphylococcus , Streptococcus spp, and Escherichia coli . Comparing the treatments, aPDT stood out, as it was able to photoinactivate all bacteria. Treatment with methylene blue photosensitizer, PBM, and aPDT induced the initial microbial reduction, but aPDT was more effective 48 h after treatment.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1435-604X
0268-8921
1435-604X
DOI:10.1007/s10103-022-03506-2