In vitro leishmanicidal activity of N -dodecyl-1,2-ethanediamine

In vitro leishmanicidal activity of N -dodecyl-1,2-ethanediamine

Abstract Polyamine biosynthesis and inhibition in parasites have been an attractive chemotherapeutic approach in the design of novel antiparasitic drugs. We study in this work the effect of N -dodecyl-1,2-ethylenediamine (NDDE) on the morphology and replication of Leishmania using macrophages cultur...

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Bibliographic Details
Published in:Biomedicine & pharmacotherapy Vol. 66; no. 3; pp. 180 - 186
Main Authors: Silva, Alexandre L. Neves, Adade, Camila M, Shoyama, Fernanda M, Neto, Cecílio Purcino S, Padrón, Thaís S, de Almeida, Mauro V, Rezende, Carlos A. de Magalhães, Silva, Claudio V, Souza, Maria A
Format: Journal Article
Language:English
Published: France Elsevier SAS 01-04-2012
Subjects:
Animals
Antiprotozoal Agents - pharmacology
Cell Line, Tumor
Cells, Cultured
Diamine
DNA Replication - drug effects
Ethylenediamines - pharmacology
Humans
Internal Medicine
Leishmania
Leishmania - drug effects
Leishmania - ultrastructure
Leishmanicidal activity
Macrophages, Peritoneal - drug effects
Medical Education
Mice
Mice, Inbred BALB C
Parasites - drug effects
Leishmanicidal activity
Diamine
Leishmania
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Summary:Abstract Polyamine biosynthesis and inhibition in parasites have been an attractive chemotherapeutic approach in the design of novel antiparasitic drugs. We study in this work the effect of N -dodecyl-1,2-ethylenediamine (NDDE) on the morphology and replication of Leishmania using macrophages cultured from the peritoneal exudate of mice infected in vitro with three species of Leishmania : Leishmania (Leishmania) amazonensis , Leishmania (Viannia) brasiliensis and Leishmania (Leishmania) chagasi . The results showed that NDDE inhibited Leishmania amastigotes multiplication into inflammatory peritoneal cells in concentrations which were not toxic to mammalian cells (0.5–1 μg/mL). An intracellular disorganization of the promastigote forms was observed by transmission electron microscopy after 3 to 24 h of treatment with 1 μg/mL NDDE, suggesting that this compound affects the viability of the parasite by an autophagy pathway.
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ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2011.10.005