Characterization of Multidrug-Resistant Staphylococcus aureus Isolates and Comparison of Methods of Susceptibility to Vancomycin

Characterization of Multidrug-Resistant Staphylococcus aureus Isolates and Comparison of Methods of Susceptibility to Vancomycin

S. aureus are among the main bacteria causing problems related to multidrug resistance in nosocomial infections. Therefore, it is necessary to carry out a reliable and rapid diagnosis for the identification of the bacteria and characterization of its susceptibility profile, especially vancomycin, wh...

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Published in:Current microbiology Vol. 79; no. 12; p. 374
Main Authors: Rosa, Danillo S., Oliveira, Samily A. S., Souza, Renata F. S., Neto, Amaro A. S., B. Cunha, Letícia S., Fernandes, Antônio W. C., Soares, Ianka F. S., Teixeira, Sarah K. R., Aquino, Samuel R., Gouveia, Gisele V., Almeida, Jackson R. G. S., Naue, Carine R., Costa, Mateus M.
Format: Journal Article
Language:English
Published: New York Springer US 01-11-2022
Springer Nature B.V
Subjects:
Antibiotics
Antimicrobial agents
Bacteria
Biofilms
Biomedical and Life Sciences
Biotechnology
Evaluation
Genes
Life Sciences
Microbiology
Minimum inhibitory concentration
Multidrug resistance
Multidrug resistant organisms
Nosocomial infection
Penicillin
Short Communication
Siderophores
Susceptibility
Vancomycin
Virulence
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Summary:S. aureus are among the main bacteria causing problems related to multidrug resistance in nosocomial infections. Therefore, it is necessary to carry out a reliable and rapid diagnosis for the identification of the bacteria and characterization of its susceptibility profile, especially vancomycin, which is an alternative treatment against multidrug-resistant (MDR) S. aureus . Thus, the goal of this study was to characterize isolates of S. aureus regarding the resistance and virulence and to check the susceptibility to vancomycin, through different methods, for comparative purposes. Seventeen antimicrobials were tested to assess the susceptibility profile. It was evaluated the presence of identification ( nuc ), resistance ( mecA and blaZ ), biofilm ( icaA and icaD ) and siderophore ( sfaD and sbnD ) genes. The susceptibility to vancomycin was evaluated by Minimum Inhibitory Concentration (MIC) by broth microdilution (BMD), E-test, commercial panel (Kit), and Phoenix equipment. Most S. aureus (93,33%) was classified as MDR. These isolates were 100% positive for nuc, mecA , icaA , icaD, and sfaD genes; 96.67% for sbnD and 33.33% for blaZ . In relation to BMD, all methods correctly classified the susceptibility of the isolates; however, regarding the exact MIC value for vancomycin, Phoenix showed agreement of 63.33%, E-test (33.33%) and Kit (26.66%). In conclusion, most of S. aureus was considered MDR. Also, they presented resistance, biofilm production, and siderophores genes, showing the pathogenic potential of these bacteria. Besides, the Phoenix test was considered the most effective, as it presents advantages, such as identification of the microorganism and a greater number of antimicrobials tested at a time.
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ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-022-03070-6