Improved synthesis of dietary triglycerides by using lipase supported on clay carriers

Background In the search for healthier and more functional foods, dietary triglycerides (TAGs) have played a prominent role in the food industry. The objective of this work was to evaluate new clay supports to immobilize lipase from Rhizopus oryzae and use it in the synthesis of TAGs. Main methods a...

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Bibliographic Details
Published in:	Biotechnology journal Vol. 17; no. 4; pp. e2100491 - n/a
Main Authors:	Pacheco, Bruna Jeanne Soares, Domingues, Otávio, Reina, Mariana Prado, Neto, Alvaro de Babtista, Andrade, Grazielle Santos Silva, de Paula, Ariela Veloso
Format:	Journal Article
Language:	English
Published:	Germany 01-04-2022
Subjects:	Clay dietary triglycerides Enzyme Stability Enzymes, Immobilized - metabolism Esterification immobilization Lipase - metabolism Rhizopus - metabolism Rhizopus oryzae lipase Triglycerides - metabolism immobilization dietary triglycerides Rhizopus oryzae lipase clay
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Summary:	Background In the search for healthier and more functional foods, dietary triglycerides (TAGs) have played a prominent role in the food industry. The objective of this work was to evaluate new clay supports to immobilize lipase from Rhizopus oryzae and use it in the synthesis of TAGs. Main methods and major results The immobilization of lipase by physical adsorption was carried out, determining the hydrolytic activity, esterification, immobilization yield, thermal stability, and kinetic and thermodynamic parameters. Afterwards, acidolysis was carried out and the product characterized as to composition, acidity index (AI), peroxide index (PI), and analysis of the sn‐2 position. The mean hydrolytic activity of free lipase was 23,568.43 ± 559.20 U g−1 and for lipase immobilized in acidified kaolin and diatomite, 1409.04 ± 51.07 and 1235.67 ± 9.56 U g–1; immobilization yield of 23.78% and 20.21% and esterification activity of 1827.99 and 1722.66 U g–1, respectively, maintaining more than 60% of the relative activity after 8 h of incubation at different temperatures. The reaction using the derivative immobilized in acidified diatomite resulted in the degree of incorporation (DI) 41.53% ± 3.99%. Conclusions and implications From the results, the potential of diatomite and kaolin as support in the immobilization of lipase for the synthesis of dietary TAGs was verified. Graphical and Lay Summary The acid treatment of clay carrier improves the esterification activity of immobilized lipase from Rhizopus oryzae (ROL). The acidified immobilized ROLs were applied in the acidolysis reaction between grape seed oil and capric acid. The higher production of MLM‐type TAGs was obtained by the immobilized ROL in acidified diatomite.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1860-6768 1860-7314
DOI:	10.1002/biot.202100491