The renal effects and initial characterization of venom from Philodryas nattereri Steindachner, 1870

The venom of the snake is a mixture of proteins and toxic peptides with several important local and systemic actions, which are similar to those occurring in snake bites. The mechanisms involved in the local and systemic actions of this venom are unknown. The aims of the work were to initial charact...

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Bibliographic Details
Published in:	Toxicology reports Vol. 1; no. C; pp. 812 - 819
Main Authors:	Nery, Marinetes Dantas de Aquino, Alves, Natacha Teresa Queiroz, de Souza Alves, Renata, de Sousa, Daniel Freire, de Menezes, Dalgimar Beserra, de Aquino Nery, Erik, de Aquino, Hermano Damasceno, de Tasso Moreira Ribeiro, Rayane, Monteiro, Helena Serra Azul
Format:	Journal Article
Language:	English
Published:	Ireland Elsevier 01-01-2014
Subjects:	Cytotoxic activity Philodryas nattereri Protein content Renal failure 2-Propenamide (PubChem CID: 6579) Serum albumin (PubChem CID: 16132) Beta-mercaptoethanol (PubChem CID: 1567) Renal failure Saline (PubChem CID 5234) Philodryas nattereri Protein content Cytotoxic activity MMT tetrazolium (PubChem CID: 64965) Tris hydrochloride (PubChem CID 93573)
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Summary:	The venom of the snake is a mixture of proteins and toxic peptides with several important local and systemic actions, which are similar to those occurring in snake bites. The mechanisms involved in the local and systemic actions of this venom are unknown. The aims of the work were to initial characterization of venom and investigate the effects of the poison in the renal perfusion system and in cultured renal tubular cells of the type MDCK (Madin-Darby canine kidney). The venom is composed majority of proteins (86.3%) and this poison promoted changes in all the evaluated renal parameters, mainly decreasing renal perfusion pressure (PP) and renal vascular resistance (RVR) and increasing urine flow (UF) and glomerular filtration rate (GFR). The most relevant result was that this venom was highly detrimental to the renal tubules independent of the PP reduction, which was shown by a decrease in sodium (Na ), potassium (K ) and chloride (Cl ) electrolyte transport in the studied concentrations. The glomeruli and tubules contain protein bodies and blood extravasation, which were observed by histological analysis. The venom of reduced viability of the MDCK cells only at high concentrations (50 and 100 μg/mL) with an IC of 169.5 μg/mL.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2214-7500 2214-7500
DOI:	10.1016/j.toxrep.2014.09.005