Insertion sequences disrupting mgrB in carbapenem-resistant Klebsiella pneumoniae strains in Brazil

•Insertion sequences disrupting mgrB were frequently detected in clinical Klebsiella pneumoniae strains.•Sequences from the IS5 family (IS903/IS903B) frequently disrupted mgrB.•Multiple sites for insertional mutation were mapped on mgrB.•ISsen4::mgrB insert was observed for the first time in K. pneu...

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Bibliographic Details
Published in:	Journal of global antimicrobial resistance. Vol. 24; pp. 53 - 57
Main Authors:	Silva, Daniely Martins da, Faria-Junior, Célio, Nery, Danielly Rocha, Oliveira, Pâmela Maria de, Silva, Lilian de Oliveira Rodrigues, Alves, Everton Giovanni, Lima, Glaura Regina de Castro e Caldo, Pereira, Alex Leite
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Ltd 01-03-2021 Elsevier
Subjects:	Insertion sequence mgrB Polymyxin resistance Polymyxin resistance mgrB Insertion sequence
Online Access:	Get full text
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Summary:	•Insertion sequences disrupting mgrB were frequently detected in clinical Klebsiella pneumoniae strains.•Sequences from the IS5 family (IS903/IS903B) frequently disrupted mgrB.•Multiple sites for insertional mutation were mapped on mgrB.•ISsen4::mgrB insert was observed for the first time in K. pneumoniae. This study aimed to characterise insertional mutations disturbing themgrB gene in carbapenem-resistant Klebsiella pneumoniae (CRKp). A total of 118 clinical CRKp isolates were surveyed for polymyxin resistance and insertion sequence (IS) elements disruptingmgrB. Of the 118 isolates, 78 (66.1%) displayed polymyxin resistance, of which 54% (42/78) hadmgrB::IS inserts. Sequencing analyses showed 13 insertion sites in mgrB. mgrB::ISSen4(IS3) was observed for the first time in CRKp. Ten different IS elements disruptedmgrB, with a predominance (76%) of IS5 sequences.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2213-7165 2213-7173
DOI:	10.1016/j.jgar.2020.11.003