Search Results - "Neoh, S H"

Quantitation of targets for PCR by use of limiting dilution by Sykes, P J, Neoh, S H, Brisco, M J, Hughes, E, Condon, J, Morley, A A

“…We describe a general method to quantitate the total number of initial targets present in a sample using limiting dilution, PCR and Poisson statistics. The DNA…”
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Outcome prediction in childhood acute lymphoblastic leukaemia by molecular quantification of residual disease at the end of induction by Brisco, M J, Condon, J, Hughes, E, Neoh, S H, Sykes, P J, Seshadri, R, Toogood, I, Waters, K, Tauro, G, Ekert, H

“…Methods to detect and quantify minimal residual disease (MRD) after chemotherapy for acute lymphoblastic leukaemia (ALL) could improve treatment by identifying…”
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Comparison of methods for assessment of minimal residual disease in childhood B-lineage acute lymphoblastic leukemia by BRISCO, M. J, SYKES, P. J, STORY, C. J, MORLEY, A. A, HUGHES, E, NEOH, S.-H, SNELL, L. E, DOLMAN, G, PENG, L.-M, TOOGOOD, I. R. G, CHENEY, K, RICE, M. S

“…The level of minimal residual disease (MRD) early in treatment of acute lymphoblastic leukemia (ALL) strongly predicts the risk of marrow relapse. As a variety…”
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Monitoring minimal residual disease in peripheral blood in B‐lineage acute lymphoblastic leukaemia by Brisco, M. J., Sykes, P. J., Hughes, E., Dolman, G., Neoh, S‐H., Peng, L‐M., Toogood, I., Morley, A. A.

“…The use of peripheral blood rather than marrow has potential advantages for monitoring minimal residual disease during the treatment of leukaemia. To determine…”
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Relationship Between Minimal Residual Disease and Outcome in Adult Acute Lymphoblastic Leukemia by Brisco, Michael J., Hughes, Elizabeth, Neoh, Sim H., Sykes, Pamela J., Bradstock, Kenneth, Enno, Arno, Szer, Jeffrey, McCaul, Kieran, Morley, Alexander A.

“…In children with acute lymphoblastic leukemia (ALL), the level of minimal residual disease (MRD) at the end of induction strongly predicts outcome, presumably…”
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Rapid detection of the factor V Leiden (1691 G > A) and haemochromatosis (845 G > A) mutation by fluorescence resonance energy transfer (FRET) and real time PCR by Neoh, S H, Brisco, M J, Firgaira, F A, Trainor, K J, Turner, D R, Morley, A A

“…A rapid method based on fluorescence resonance energy transfer (FRET) and real time polymerase chain reaction (PCR) was used to identify the haemochromatosis…”
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Effect of the Philadelphia chromosome on minimal residual disease in acute lymphoblastic leukemia by BRISCO, M. J, SYKES, P. J, MORLEY, A. A, DOLMAN, G, NEOH, S.-H, HUGHES, E, PENG, L.-M, TAURO, G, EKERT, H, TOOGOOD, I, BRADSTOCK, K

“…The Philadelphia translocation is associated with a poor prognosis in adults and children with acute lymphoblastic leukemia, even though the majority of…”
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The use of monoclonal gene rearrangement for detection of minimal residual disease in acute lymphoblastic leukemia of childhood by SYKES, P. J, SNELL, L. E, BRISCOL, M. J, NEOH, S.-H, HUGHES, E, DOLMAN, G, PENG, L.-M, BENNETT, A, TOOGOOD, I, MORLEY, A. A

“…Sensitive quantification of minimal residual disease (MRD) using the polymerase chain reaction (PCR) is strongly predictive of outcome in childhood acute…”
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Detection and quantitation of neoplastic cells in acute lymphoblastic leukaemia, by use of the polymerase chain reaction by Brisco, M J, Condon, J, Sykes, P J, Neoh, S H, Morley, A A

“…We report a simple and robust method for sensitive quantitation of leukaemic cells in acute lymphocytic leukaemia. Chain determining region 3 (CDR3) of the…”
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Leukaemia presenting as marrow hypoplasia: molecular detection of the leukaemic clone at the time of initial presentation by Morley, A. A., Brisco, M. J., Rice, M., Snell, L., Peng, L‐M., Hughes, E., Neoh, S‐H., Sykes, P. J.

“…Occasional cases of transient marrow hypoplasia in childhood evolve into acute leukaemia. We studied two children who presented with marrow hypoplasia…”
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Parotid abscess in a late premature infant: a case report by Zurina, Z, Wong, H L, Jasminder, K, Neoh, S H, Cheah, I G S

“…Parotid abscess is uncommon in neonates. It is frequently related to prematurity, prolonged gavage feeding and dehydration. We report a case of a late preterm…”
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Early resistance to therapy during induction in childhood acute lymphoblastic leukemia by BRISCO, Michael J, SYKES, Pamela J, DOLMAN, Gaynor, HUGHES, Elizabeth, NEOH, Sim-Hee, LIMING PENG, SNELL, Lesley E, TOOGOOD, Ian R. G, RICE, Michael S, MORLEY, Alexander A

“…Many patients with acute lymphoblastic leukemia (ALL) are not cured by current therapy because of the development of drug resistance. It is not clear when…”
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Minimal residual disease in childhood acute lymphoblastic leukaemia quantified by aspirate and trephine : is the disease multifocal? by SYKES, P. J, BRISCO, M. J, HUGHES, E, SNELL, L. E, DOLMAN, G, NEOH, S.-H, PENG, L.-M, TOOGOOD, I, VENABLES, W. N, MORLEY, A. A

“…The level of minimal residual disease (MRD) in marrow early in treatment strongly predicts outcome in childhood acute lymphoblastic leukaemia (ALL). Using PCR…”
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A comparison of the sensitivity of immunoperoxidase staining methods with high-sensitivity fluorescence flow cytometry-antibody quantitation on the cell surface by Coventry, BJ, Neoh, SH, Mantzioris, BX, Skinner, JM, Zola, H, Bradley, J

“…Surface molecules present in low copy numbers can be detected with high-sensitivity fluorescence flow cytometry. Many cells previously thought not to express…”
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Detection by immunofluorescence of surface molecules present in low copy numbers. High sensitivity staining and calibration of flow cytometer by Zola, H, Neoh, S H, Mantzioris, B X, Webster, J, Loughnan, M S

“…Receptors for lymphokines and growth factors are present on cell surfaces often at concentrations of 100-500 copies per cell. Although conventional…”
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The purification of mouse monoclonal antibodies from ascitic fluid by Neoh, S H, Gordon, C, Potter, A, Zola, H

“…A method is described for the purification of monoclonal antibody from mouse ascitic fluid. The fluid is clarified and the lipid removed using silicon dioxide…”
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Monoclonal antibody purification: choice of method and assessment of purity and yield by Zola, H, Neoh, S H

“…In this article we discuss our choices of monoclonal antibody separation methods for the applications which face us most frequently. We explain the rationale…”
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The p24 leucocyte membrane antigen: modulation associated with lymphocyte activation and differentiation by Zola, H, Furness, V, Barclay, S, Zowtyj, H, Smith, M, Melo, J V, Neoh, S H, Bradley, J

“…Monoclonal antibodies of the CD9 cluster recognize a 24 kD protein (p24) found on platelets and endothelium, and expressed by lymphocytes at restricted stages…”
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Markers of differentiated B cell leukaemia: CD22 antibodies and FMC7 react with different molecules by Zola, H, Neoh, S H, Potter, A, Melo, J V, De Oliveria, M S, Catovsky, D

“…FMC7, a monoclonal antibody used extensively to characterize B cell leukaemias of differentiated phenotype (prolymphocytic, hairy cell, and similar leukaemias)…”
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Monoclonal antibodies to Toxoplasma cell membrane surface antigens protect mice from toxoplasmosis by Johnson, A M, McDonald, P J, Neoh, S H

“…Groups of mice were given an intraperitoneal injection of one of six monoclonal antibodies to Toxoplasma gondii, a mixture of equal amounts of five monoclonal…”
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