Search Results - "Nel, Andrew"

MetaNovo: An open-source pipeline for probabilistic peptide discovery in complex metaproteomic datasets by Potgieter, Matthys G, Nel, Andrew J M, Fortuin, Suereta, Garnett, Shaun, Wendoh, Jerome M, Tabb, David L, Mulder, Nicola J, Blackburn, Jonathan M

“…Microbiome research is providing important new insights into the metabolic interactions of complex microbial ecosystems involved in fields as diverse as the…”
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The cyanobacterial lectin, microvirin-N, enhances the specificity and sensitivity of lipoarabinomannan-based TB diagnostic tests by van der Horst, Megan, Karamchand, Leshern, Bauer, Westley S, Nel, Andrew J M, Blackburn, Jonathan M, Wright, David W

“…Tuberculosis (TB) is one of the top ten causes of death globally, despite being treatable. The eradication of TB disease requires, amongst others, diagnostic…”
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Comparative Reevaluation of FASP and Enhanced FASP Methods by LC–MS/MS by Nel, Andrew J. M, Garnett, Shaun, Blackburn, Jonathan M, Soares, Nelson C

“…Filter-aided sample preparation is a proteomic technique for the preparation and on column proteolysis of proteins. Recently an enhanced FASP protocol was…”
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Comparison between the proteome of Escherichia coli single colony and during liquid culture by Fortuin, Suereta, Nel, Andrew J.M., Blackburn, Jonathan M., Soares, Nelson C.

“…Most bacterial proteomic studies done to date utilise bacterial cells harvested from liquid culture media. However, it is widely accepted that many important…”
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Epitope Coverage of Anti-SARS-CoV-2 Nucleocapsid IgA and IgG Antibodies Correlates with Protection against Re-Infection by New Variants in Subsequent Waves of the COVID-19 Pandemic by Mullins, Michelle O, Smith, Muneerah, Maboreke, Hazel, Nel, Andrew J M, Ntusi, Ntobeko A B, Burgers, Wendy A, Blackburn, Jonathan M

“…The COVID-19 pandemic continues to affect individuals across the globe, with some individuals experiencing more severe disease than others. The relatively high…”
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Identification of Novel Physiological Substrates of Mycobacterium bovis BCG Protein Kinase G (PknG) by Label-free Quantitative Phosphoproteomics by Nakedi, Kehilwe C., Calder, Bridget, Banerjee, Mousumi, Giddey, Alexander, Nel, Andrew J.M., Garnett, Shaun, Blackburn, Jonathan M., Soares, Nelson C.

“…Mycobacterial Ser/Thr kinases play a critical role in bacterial physiology and pathogenesis. Linking kinases to the substrates they phosphorylate in vivo,…”
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Humoral immunoprofiling identifies novel biomarkers and an immune suppressive autoantibody phenotype at the site of disease in pancreatic ductal adenocarcinoma by Maimela, Pamela Winnie M, Smith, Muneerah, Nel, Andrew J M, Bernam, Suba Dharshanan P, Jonas, Eduard G, Blackburn, Jonathan M

“…Pancreatic ductal adenocarcinoma (PDAC) is a heterogeneous cancer, with minimal response to therapeutic intervention and with 85% of cases diagnosed at an…”
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Cell wall enrichment unveils proteomic changes in the cell wall during treatment of Mycobacterium smegmatis with sub-lethal concentrations of rifampicin by Hermann, Clemens, Giddey, Alexander D., Nel, Andrew J.M., Soares, Nelson C., Blackburn, Jonathan M.

“…Understanding the cell wall of mycobacteria is crucial for improving drug design or identifying new antigens suitable to vaccination. Yet this remains…”
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Global proteome and phosphoproteome dynamics indicate novel mechanisms of vitamin C induced dormancy in Mycobacterium smegmatis by Albeldas, Claudia, Ganief, Naadir, Calder, Bridget, Nakedi, Kehilwe C., Garnett, Shaun, Nel, Andrew J.M., Blackburn, Jonathan M., Soares, Nelson C.

“…Vitamin C has been found to affect mycobacteria in multiple ways, including increasing susceptibility to antimicrobial drugs, inducing dormancy, and having a…”
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A temporal proteome dynamics study reveals the molecular basis of induced phenotypic resistance in Mycobacterium smegmatis at sub-lethal rifampicin concentrations by Giddey, Alexander D., de Kock, Elise, Nakedi, Kehilwe C., Garnett, Shaun, Nel, Andrew J. M., Soares, Nelson C., Blackburn, Jonathan M.

“…In the last 40 years only one new antitubercular drug has been approved, whilst resistance to current drugs, including rifampicin, is spreading. Here, we used…”
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Proteomic comparison of three clinical diarrhoeagenic drug-resistant Escherichia coli isolates grown on CHROMagar™STEC media by Kalule, John Bosco, Fortuin, Suereta, Calder, Bridget, Robberts, Lourens, Keddy, Karen H., Nel, Andrew J.M., Garnett, Shaun, Nicol, Mark, Warner, Digby F., Soares, Nelson C., Blackburn, Jonathan M.

“…Shiga-toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) are key diarrhoea-causing foodborne pathogens. We used proteomics to…”
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HIV Nef enhances the expression of oncogenic c-MYC and activation-induced cytidine deaminase in Burkitt lymphoma cells, promoting genomic instability by Mdletshe, Nontlantla, Nel, Andrew, Shires, Karen, Mowla, Shaheen

“…Abstract Background Non-Hodgkin lymphoma is of high prevalence among HIV-infected people. In particular, the incidence of HIV-associated Burkitt lymphoma (BL)…”
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Comparative Ser/Thr/Tyr phosphoproteomics between two mycobacterial species: the fast growing Mycobacterium smegmatis and the slow growing Mycobacterium bovis BCG by Nakedi, Kehilwe C, Nel, Andrew J M, Garnett, Shaun, Blackburn, Jonathan M, Soares, Nelson C

“…Ser/Thr/Tyr protein phosphorylation plays a critical role in regulating mycobacterial growth and development. Understanding the mechanistic link between…”
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Proteogenomic Analysis of Mycobacterium smegmatis Using High Resolution Mass Spectrometry by Potgieter, Matthys G, Nakedi, Kehilwe C, Ambler, Jon M, Nel, Andrew J M, Garnett, Shaun, Soares, Nelson C, Mulder, Nicola, Blackburn, Jonathan M

“…Biochemical evidence is vital for accurate genome annotation. The integration of experimental data collected at the proteome level using high resolution mass…”
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Age, Disease Severity and Ethnicity Influence Humoral Responses in a Multi-Ethnic COVID-19 Cohort by Smith, Muneerah, Abdesselem, Houari B, Mullins, Michelle, Tan, Ti-Myen, Nel, Andrew J M, Al-Nesf, Maryam A Y, Bensmail, Ilham, Majbour, Nour K, Vaikath, Nishant N, Naik, Adviti, Ouararhni, Khalid, Mohamed-Ali, Vidya, Al-Maadheed, Mohammed, Schell, Darien T, Baros-Steyl, Seanantha S, Anuar, Nur D, Ismail, Nur H, Morris, Priscilla E, Mamat, Raja N R, Rosli, Nurul S M, Anwar, Arif, Ellan, Kavithambigai, Zain, Rozainanee M, Burgers, Wendy A, Mayne, Elizabeth S, El-Agnaf, Omar M A, Blackburn, Jonathan M

“…The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a…”
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Longitudinal IgA and IgG Response, and ACE2 Binding Blockade, to Full-Length SARS-CoV-2 Spike Protein Variants in a Population of Black PLWH Vaccinated with ChAdOx1 nCoV-19 by Smith, Muneerah, Kwatra, Gaurav, Izu, Alane, Nel, Andrew, Cutland, Clare, Ahmed, Khatja, Baillie, Vicky, Barnabas, Shaun, Bhorat, Qasim, Briner, Carmen, Lazarus, Erica, Dheda, Keertan, Fairlie, Lee, Koen, Anthonet, Madhi, Shabir, Blackburn, Jonathan M

“…Vaccines against SARS-CoV-2 have been pivotal in overcoming the COVID-19 pandemic yet understanding the subsequent outcomes and immunological effects remain…”
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Liquid chromatography mass spectrometry-based proteomics of Escherichia coli single colony by Fortuin, Suereta, Iradukunda, John, Nel, Andrew JM, Blackburn, Jonathan M, Soares, Nelson C

“…The Escherichia coli proteome is the most extensively characterized and studied of all prokaryotic proteomes. Despite this, large scale bacterial proteomics…”
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Gel-free sample preparation techniques and bioinformatic enrichment analysis to in depth characterise the cell wall proteome of mycobacteria by Hermann, Clemens, Giddey, Alexander D., Nel, Andrew J.M., Soares, Nelson C., Blackburn, Jonathan M.

“…[Display omitted] The comprehensive characterisation of the cell wall proteome of mycobacteria is of considerable relevance to both the discovery of new drug…”
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IL-4 subverts mycobacterial containment in Mycobacterium tuberculosis -infected human macrophages by Pooran, Anil, Davids, Malika, Nel, Andrew, Shoko, Aubrey, Blackburn, Jonathan, Dheda, Keertan

“…Protective immunity against is poorly understood. The role of interleukin (IL)-4, the archetypal T-helper type 2 (Th2) cytokine, in the immunopathogenesis of…”
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Design and use of mouse control DNA for DNA biomarker extraction and PCR detection from urine: Application for transrenal Mycobacterium tuberculosis DNA detection by Bordelon, Hali, Ricks, Keersten M., Pask, Megan E., Russ, Patricia K., Solinas, Francesca, Baglia, Mark L., Short, Philip A., Nel, Andrew, Blackburn, Jonathan, Dheda, Keertan, Zamudio, Carlos, Cáceres, Tatiana, Wright, David W., Haselton, Frederick R., Pettit, April C.

“…Urine samples are increasingly used for diagnosing infections including Escherichia coli, Ebola virus, and Zika virus. However, extraction and concentration of…”
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