Getah Virus Infection Rapidly Causes Testicular Damage and Decreases Sperm Quality in Male Mice
Getah virus (GETV) is a zoonotic arbovirus that can cause infection in many animals. It can cause pyrexia and reproductive losses in animals. The objective of the study was to explore the effects of GETV on male reproductive ability. Male mice were injected with 100 × TCID /0.1 ml in a volume of 100...
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| Published in: | Frontiers in veterinary science Vol. 9; p. 883607 |
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| Main Authors: | , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Switzerland
Frontiers Media S.A
25-04-2022
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Getah virus (GETV) is a zoonotic arbovirus that can cause infection in many animals. It can cause pyrexia and reproductive losses in animals. The objective of the study was to explore the effects of GETV on male reproductive ability. Male mice were injected with 100 × TCID
/0.1 ml in a volume of 100-μL GETV in their hindquarter muscle, resulting in decreased semen quality and testicular histopathological changes, and the virus was detected in the testes. At 0.5 dpi (day post-infection), male mice showed decreased sperm density, motility, and decreased serum testosterone concentration, an increased sperm malformation rate, vacuoles in spermatogonial cells/spermatocytes in spermatogenic tubules, and the highest virus copies in testis. At 2 dpi, the sperm density and motility reached the lowest value of 3.99 × 10
/ml and 62.03%, and the malformation rate reached 43.67%. At 28 dpi, the sperm indexes of the experimental group gradually approached that of the control group, but there were still significant differences. Since then, histopathological changes have worsened, with the most severe histopathological changes at 7 dpi and gradual recovery. Up to 14 dpi, the virus was detected by qRT-PCR and immunohistochemistry, which showed that the virus was only present in the testicular interstitium. GETV infection can rapidly enter the testis of mice and reduce the semen quality of mice, which needs to be paid attention to in the prevention and control of GETV. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors have contributed equally to this work and shared co-first authorship Edited by: Zhenhai Chen, College of Veterinary Medicine, Yangzhou University, China Reviewed by: Uladzimir Karniychuk, University of Saskatchewan, Canada; Tereza Cristina Cardoso, Universidade Estadual de São Paulo, Brazil This article was submitted to Veterinary Infectious Diseases, a section of the journal Frontiers in Veterinary Science |
| ISSN: | 2297-1769 2297-1769 |
| DOI: | 10.3389/fvets.2022.883607 |