Search Results - "Negoro, Seiji"
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X‐ray crystallographic and mutational analysis of the NylC precursor: catalytic mechanism of autocleavage and substrate hydrolysis of nylon hydrolase
Published in The FEBS journal (01-07-2023)“…Nylon hydrolase (NylC), a member of the N‐terminal nucleophile (Ntn) hydrolase superfamily, is responsible for the degradation of various aliphatic nylons,…”
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Structural and functional characterization of nylon hydrolases
Published in Methods in enzymology (2021)“…Biodegradation of synthetic polymers is recognized as a useful way to reduce their environmental load and pollution, loss of natural resources, extensive…”
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Degradation Potential of the Nonylphenol Monooxygenase of Sphingomonas sp. NP5 for Bisphenols and Their Structural Analogs
Published in Microorganisms (Basel) (19-02-2020)“…The nonylphenol-degrading bacterium sp. strain NP5 has a very unique monooxygenase that can attack a wide range of 4-alkylphenols with a branched side chain…”
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Metabolic pathway of 6-aminohexanoate in the nylon oligomer-degrading bacterium Arthrobacter sp. KI72: identification of the enzymes responsible for the conversion of 6-aminohexanoate to adipate
Published in Applied microbiology and biotechnology (01-01-2018)“…Arthrobacter sp. strain KI72 grows on a 6-aminohexanoate oligomer, which is a by-product of nylon-6 manufacturing, as a sole source of carbon and nitrogen. We…”
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Biosynthesis-inspired deracemizative production of d-luciferin by combining luciferase and thioesterase
Published in Biochimica et biophysica acta. General subjects (01-08-2017)“…Due to the strict enantioselectivity of firefly luciferase, only d-luciferin can be used as a substrate for bioluminescence reactions. Unfortunately, luciferin…”
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Function of a Glutamine Synthetase-Like Protein in Bacterial Aniline Oxidation via γ-Glutamylanilide
Published in Journal of Bacteriology (01-10-2013)“…Acinetobacter sp. strain YAA has five genes (atdA1 to atdA5) involved in aniline oxidation as a part of the aniline degradation gene cluster. From sequence…”
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Unraveling the degradation of artificial amide bonds in nylon oligomer hydrolase: from induced-fit to acylation processes
Published in Physical chemistry chemical physics : PCCP (01-01-2015)“…To elucidate how the nylon oligomer hydrolase (NylB) acquires its peculiar degradation activity towards non-biological amide bonds, we inspected the underlying…”
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Characterization of the 3-methyl-4-nitrophenol degradation pathway and genes of Pseudomonas sp. strain TSN1
Published in Journal of bioscience and bioengineering (01-09-2018)“…3-Methyl-4-nitrophenol (3M4NP) is formed in soil as a hydrolysis product of fenitrothion, one of the major organophosphorus pesticides. A Pseudomonas strain…”
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9
Structural basis of the correct subunit assembly, aggregation, and intracellular degradation of nylon hydrolase
Published in Scientific reports (27-06-2018)“…Nylon hydrolase (NylC) is initially expressed as an inactive precursor (36 kDa). The precursor is cleaved autocatalytically at Asn266/Thr267 to generate an…”
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Enzymatic hydrolysis of nylons: quantification of the reaction rate of nylon hydrolase for thin-layered nylons
Published in Applied microbiology and biotechnology (01-10-2014)“…Nylon hydrolase degrades various aliphatic nylons, including nylon-6 and nylon-66. We synthesized a nylon-66 copolymer (M w = 22,900, M ₙ = 7,400), in which a…”
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11
Mechanism of 4-Nitrophenol Oxidation in Rhodococcus sp. Strain PN1: Characterization of the Two-Component 4-Nitrophenol Hydroxylase and Regulation of Its Expression
Published in Journal of Bacteriology (01-11-2008)“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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12
Distribution of chitin/chitosan-like bioflocculant-producing potential in the genus Citrobacter
Published in Applied microbiology and biotechnology (01-11-2013)“…Some strains belonging to the genera Citrobacter and Enterobacter have been reported to produce chitin/chitosan-like bioflocculants (BFs) from acetate. In this…”
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Biosynthetic Pathway and Genes of Chitin/Chitosan-Like Bioflocculant in the Genus Citrobacter
Published in Polymers (27-02-2018)“…Chitin/chitosan, one of the most abundant polysaccharides in nature, is industrially produced as a powder or flake form from the exoskeletons of crustaceans…”
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Draft Genome Sequence of the Nylon Oligomer-Degrading Bacterium Arthrobacter sp. Strain KI72
Published in Genome announcements (Washington, DC) (27-04-2017)“…We report here the 4.6-Mb genome sequence of a nylon oligomer-degrading bacterium, sp. strain KI72. The draft genome sequence of strain KI72 consists of…”
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Mutations affecting the internal equilibrium of the reaction catalyzed by 6‐aminohexanoate‐dimer hydrolase
Published in FEBS letters (01-09-2016)“…The enzyme 6‐aminohexanoate‐dimer hydrolase catalyzes amide synthesis. The yield of this reverse reaction in 90% t‐butyl alcohol was found to vary drastically…”
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Interconversion of ketoprofen recognition in firefly luciferase-catalyzed enantioselective thioesterification reaction using from Pylocoeria miyako (PmL) and Hotaria parvura (HpL) just by mutating two amino acid residues
Published in Journal of biotechnology (01-11-2013)“…We identified the critical amino acid residues that determine the substrate recognition pattern of thioesterification reaction toward ketoprofen using two…”
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17
6-Aminohexanoate Oligomer Hydrolases from the Alkalophilic Bacteria Agromyces sp. Strain KY5R and Kocuria sp. Strain KY2
Published in Applied and Environmental Microbiology (01-11-2007)“…Alkalophilic, nylon oligomer-degrading strains, Agromyces sp. and Kocuria sp., were isolated from the wastewater of a nylon-6 factory and from activated sludge…”
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18
Molecular dynamics studies on the mutational structures of a nylon-6 byproduct-degrading enzyme
Published in Chemical physics letters (29-04-2011)“…[Display omitted] ► Wild-type and mutant structures of nylon-6 byproduct-degrading enzyme are determined. ► We sampled 25 000 data from 10–35 ns simulations to…”
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Confirmation of color determination factors for Ser286 derivatives of firefly luciferase from Luciola cruciata (LUC-G)
Published in Journal of molecular catalysis. B, Enzymatic (01-03-2013)“…We constructed a mutant library of Japanese firefly Luciola cruciata luciferase (LUC-G) at position S286 and performed conclusive characterization of these…”
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Enantiodifferentiation of ketoprofen by Japanese firefly luciferase from Luciola lateralis
Published in Journal of molecular catalysis. B, Enzymatic (01-05-2011)“…A Japanese firefly luciferase from Luciola lateralis (LUC-H) has an enantioselective acyl-CoA synthetase activity toward ketoprofen. In this article, we…”
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