Search Results - "Needham, Sarah R"

EGFR oligomerization organizes kinase-active dimers into competent signalling platforms by Needham, Sarah R., Roberts, Selene K., Arkhipov, Anton, Mysore, Venkatesh P., Tynan, Christopher J., Zanetti-Domingues, Laura C., Kim, Eric T., Losasso, Valeria, Korovesis, Dimitrios, Hirsch, Michael, Rolfe, Daniel J., Clarke, David T., Winn, Martyn D., Lajevardipour, Alireza, Clayton, Andrew H. A., Pike, Linda J., Perani, Michela, Parker, Peter J., Shan, Yibing, Shaw, David E., Martin-Fernandez, Marisa L.

“…Epidermal growth factor receptor (EGFR) signalling is activated by ligand-induced receptor dimerization. Notably, ligand binding also induces EGFR…”
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Affimer proteins are versatile and renewable affinity reagents by Tiede, Christian, Bedford, Robert, Heseltine, Sophie J, Smith, Gina, Wijetunga, Imeshi, Ross, Rebecca, AlQallaf, Danah, Roberts, Ashley Pe, Balls, Alexander, Curd, Alistair, Hughes, Ruth E, Martin, Heather, Needham, Sarah R, Zanetti-Domingues, Laura C, Sadigh, Yashar, Peacock, Thomas P, Tang, Anna A, Gibson, Naomi, Kyle, Hannah, Platt, Geoffrey W, Ingram, Nicola, Taylor, Thomas, Coletta, Louise P, Manfield, Iain, Knowles, Margaret, Bell, Sandra, Esteves, Filomena, Maqbool, Azhar, Prasad, Raj K, Drinkhill, Mark, Bon, Robin S, Patel, Vikesh, Goodchild, Sarah A, Martin-Fernandez, Marisa, Owens, Ray J, Nettleship, Joanne E, Webb, Michael E, Harrison, Michael, Lippiat, Jonathan D, Ponnambalam, Sreenivasan, Peckham, Michelle, Smith, Alastair, Ferrigno, Paul Ko, Johnson, Matt, McPherson, Michael J, Tomlinson, Darren Charles

“…Molecular recognition reagents are key tools for understanding biological processes and are used universally by scientists to study protein expression,…”
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Fluorescence and phosphorescence lifetime imaging reveals a significant cell nuclear viscosity and refractive index changes upon DNA damage by Clancy, Ellen, Ramadurai, Siva, Needham, Sarah R., Baker, Karen, Eastwood, Tara A., Weinstein, Julia A., Mulvihill, Daniel P., Botchway, Stanley W.

“…Cytoplasmic viscosity is a crucial parameter in determining rates of diffusion-limited reactions. Changes in viscosity are associated with several diseases,…”
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The use of NADH anisotropy to investigate mitochondrial cristae alignment by Smith, Holly. E., Mackenzie, Alasdair M., Seddon, Chloe, Mould, Rhys, Kalampouka, Ifi, Malakar, Partha, Needham, Sarah R., Beis, Konstantinos, Bell, Jimmy D., Nunn, Alistair, Botchway, Stanley W.

“…Life may be expressed as the flow of electrons, protons, and other ions, resulting in large potential difference. It is also highly photo-sensitive, as a large…”
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Drug-resistant EGFR mutations promote lung cancer by stabilizing interfaces in ligand-free kinase-active EGFR oligomers by Iyer, R. Sumanth, Needham, Sarah R., Galdadas, Ioannis, Davis, Benjamin M., Roberts, Selene K., Man, Rico C. H., Zanetti-Domingues, Laura C., Clarke, David T., Fruhwirth, Gilbert O., Parker, Peter J., Rolfe, Daniel J., Gervasio, Francesco L., Martin-Fernandez, Marisa L.

“…The Epidermal Growth Factor Receptor (EGFR) is frequently found to be mutated in non-small cell lung cancer. Oncogenic EGFR has been successfully targeted by…”
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Measuring EGFR separations on cells with ~10 nm resolution via fluorophore localization imaging with photobleaching by Needham, Sarah R, Hirsch, Michael, Rolfe, Daniel J, Clarke, David T, Zanetti-Domingues, Laura C, Wareham, Richard, Martin-Fernandez, Marisa L

“…Detecting receptor dimerisation and other forms of clustering on the cell surface depends on methods capable of determining protein-protein separations with…”
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A systematic investigation of differential effects of cell culture substrates on the extent of artifacts in single-molecule tracking by Zanetti-Domingues, Laura C, Martin-Fernandez, Marisa L, Needham, Sarah R, Rolfe, Daniel J, Clarke, David T

“…Single-molecule techniques are being increasingly applied to biomedical investigation, notwithstanding the numerous challenges they pose in terms of…”
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Structure-function relationships and supramolecular organization of the EGFR (epidermal growth factor receptor) on the cell surface by Needham, Sarah R, Zanetti-Domingues, Laura C, Hirsch, Michael, Rolfe, Daniel J, Tynan, Christopher J, Roberts, Selene K, Martin-Fernandez, Marisa L, Clarke, David T

“…Dimerization and higher-order oligomerization are believed to play an important role in the activation of the EGFR (epidermal growth factor receptor)…”
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Determining the geometry of oligomers of the human epidermal growth factor family on cells with <10 nm resolution by Needham, Sarah R, Zanetti-Domingues, Laura C, Scherer, Kathrin M, Hirsch, Michael, Rolfe, Daniel J, Roberts, Selene K, Martin-Fernandez, Marisa L, Clarke, David T, Tynan, Christopher J

“…There is a limited range of methods available to characterize macromolecular organization in cells on length scales from 5-50 nm. We review methods currently…”
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Ultra‐Small Air‐Stable Triplet‐Triplet Annihilation Upconversion Nanoparticles for Anti‐Stokes Time‐Resolved Imaging by Zhang, Bolong, Richards, Kieran D., Jones, Beatrice E., Collins, Abigail R., Sanders, Rosie, Needham, Sarah R., Qian, Pu, Mahadevegowda, Amoghavarsha, Ducati, Caterina, Botchway, Stanley W., Evans, Rachel C.

“…Image contrast is often limited by background autofluorescence in steady‐state bioimaging microscopy. Upconversion bioimaging can overcome this by shifting the…”
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Towards cloud-enabled fully automated end-to-end single molecule imaging by Davis, Benjamin M., Needham, Sarah R., Iyer, Sumanth, Roberts, Selene K., Domingues, Laura C. Zanetti, Martin-Fernandez, Marisa, Rolfe, Daniel J.

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Back Cover: Ultra‐Small Air‐Stable Triplet‐Triplet Annihilation Upconversion Nanoparticles for Anti‐Stokes Time‐Resolved Imaging (Angew. Chem. Int. Ed. 47/2023) by Zhang, Bolong, Richards, Kieran D., Jones, Beatrice E., Collins, Abigail R., Sanders, Rosie, Needham, Sarah R., Qian, Pu, Mahadevegowda, Amoghavarsha, Ducati, Caterina, Botchway, Stanley W., Evans, Rachel C.

“…Anti‐Stokes fluorescence lifetime imaging in living cells was demonstrated by Rachel C. Evans et al. in their Research Article (e202308602). This was achieved…”
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Affimers proteins are versatile and renewable affinity reagents by Tiede, Christian, Bedford, Robert, Heseltine, Sophie J, Smith, Gina, Wijetunga, Imeshi, Ross, Rebecca, AlQallaf, Danah, Roberts, Ashley PE, Balls, Alexander, Curd, Alistair, Hughes, Ruth E, Martin, Heather, Needham, Sarah R, Zanetti-Domingues, Laura C, Sadigh, Yashar, Peacock, Thomas P, Tang, Anna A, Gibson, Naomi, Kyle, Hannah, Platt, Geoffrey W, Ingram, Nicola, Taylor, Thomas, Coletta, Louise P, Manfield, Iain, Knowles, Margaret, Bell, Sandra, Esteves, Filomena, Maqbool, Azhar, Prasad, Raj K, Drinkhill, Mark, Bon, Robin S, Patel, Vikesh, Goodchild, Sarah A, Martin-Fernandez, Marisa, Owens, Ray J, Nettleship, Joanne E, Webb, Michael E, Harrison, Michael, Lippiat, Jonathan D, Ponnambalam, Sreenivasan, Peckham, Michelle, Smith, Alastair, Ferrigno, Paul Ko, Johnson, Matt, McPherson, Michael J, Tomlinson, Darren Charles

“…Molecular recognition reagents are key tools for understanding biological processes and are used universally by scientists to study protein expression,…”
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The architecture of EGFR’s basal complexes reveals autoinhibition mechanisms in dimers and oligomers by Zanetti-Domingues, Laura C., Korovesis, Dimitrios, Needham, Sarah R., Tynan, Christopher J., Sagawa, Shiori, Roberts, Selene K., Kuzmanic, Antonija, Ortiz-Zapater, Elena, Jain, Purvi, Roovers, Rob C., Lajevardipour, Alireza, van Bergen en Henegouwen, Paul M. P., Santis, George, Clayton, Andrew H. A., Clarke, David T., Gervasio, Francesco L., Shan, Yibing, Shaw, David E., Rolfe, Daniel J., Parker, Peter J., Martin-Fernandez, Marisa L.

“…Our current understanding of epidermal growth factor receptor (EGFR) autoinhibition is based on X-ray structural data of monomer and dimer receptor fragments…”
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Directly imaging the localisation and photosensitization properties of the pan-mTOR inhibitor, AZD2014, in living cancer cells by Ahmed, Abdullah R., Candeo, Alessia, D'Abrantes, Sofia, Needham, Sarah R., Yadav, Rahul B., Botchway, Stanley W., Parker, Anthony W.

“…The range of cellular functions the mechanistic target of rapamycin (mTOR) protein performs makes it an attractive drug target for cancer therapy. However, the…”
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Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution by Wang, Lin, Bateman, Benji, Zanetti-Domingues, Laura C., Moores, Amy N., Astbury, Sam, Spindloe, Christopher, Darrow, Michele C., Romano, Maria, Needham, Sarah R., Beis, Konstantinos, Rolfe, Daniel J., Clarke, David T., Martin-Fernandez, Marisa L.

“…Super-resolution fluorescence microscopy plays a crucial role in our understanding of cell structure and function by reporting cellular ultrastructure with…”
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Ultra‐Small Air‐Stable Triplet‐Triplet Annihilation Upconversion Nanoparticles for Anti‐Stokes Time‐Resolved Imaging by Zhang, Bolong, Richards, Kieran D., Jones, Beatrice E., Collins, Abigail R., Sanders, Rosie, Needham, Sarah R., Qian, Pu, Mahadevegowda, Amoghavarsha, Ducati, Caterina, Botchway, Stanley W., Evans, Rachel C.

“…Image contrast is often limited by background autofluorescence in steady‐state bioimaging microscopy. Upconversion bioimaging can overcome this by shifting the…”
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Rücktitelbild: Ultra‐Small Air‐Stable Triplet‐Triplet Annihilation Upconversion Nanoparticles for Anti‐Stokes Time‐Resolved Imaging (Angew. Chem. 47/2023) by Zhang, Bolong, Richards, Kieran D., Jones, Beatrice E., Collins, Abigail R., Sanders, Rosie, Needham, Sarah R., Qian, Pu, Mahadevegowda, Amoghavarsha, Ducati, Caterina, Botchway, Stanley W., Evans, Rachel C.

“…Anti‐Stokes fluorescence lifetime imaging in living cells was demonstrated by Rachel C. Evans et al. in their Research Article (e202308602). This was achieved…”
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A tale of the epidermal growth factor receptor: The quest for structural resolution on cells by Tynan, Christopher J., Lo Schiavo, Valentina, Zanetti-Domingues, Laura, Needham, Sarah R., Roberts, Selene K., Hirsch, Michael, Rolfe, Daniel J., Korovesis, Dimitrios, Clarke, David T., Martin-Fernandez, Marisa L.

“…•We describe how key concepts in EGFR research have evolved over the preceding decades.•Atomic resolution structures provided by X-ray crystallography provide…”
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Nanometric molecular separation measurements by single molecule photobleaching by Webb, Stephen E.D., Hirsch, Michael, Needham, Sarah R., Coles, Benjamin C., Scherer, Kathrin M., Roberts, Selene K., Zanetti-Domingues, Laura C., Tynan, Christopher J., Martin-Fernandez, Marisa L., Rolfe, Daniel J.

“…•Fluorophore localisation imaging with photobleaching can measure 5–50nm molecular separations.•Unliganded epidermal growth factor receptors exist as oligomers…”
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