Bone marrow contributes to renal parenchymal turnover and regeneration

In order to establish whether extra‐renal cells contribute to the turnover and repair of renal tissues, this study examined kidneys of female mice that had received a male bone marrow transplant and kidney biopsies from male patients who had received kidney transplants from female donors. By using i...

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Bibliographic Details
Published in:	The Journal of pathology Vol. 195; no. 2; pp. 229 - 235
Main Authors:	Poulsom, Richard, Forbes, Stuart J., Hodivala-Dilke, Kairbaan, Ryan, Eoin, Wyles, Susannah, Navaratnarasah, Sobana, Jeffery, Rosemary, Hunt, Toby, Alison, Malcolm, Cook, Terence, Pusey, Charles, Wright, Nicholas A.
Format:	Journal Article
Language:	English
Published:	Chichester, UK John Wiley & Sons, Ltd 01-09-2001 Wiley
Subjects:	Animals Biological and medical sciences Biopsy bone marrow stem cells Bone Marrow Transplantation - pathology Cell Differentiation Epithelial Cells - pathology Female Hematopoietic Stem Cells - pathology Humans In Situ Hybridization Kidney - pathology Kidney - physiology Kidney Transplantation - pathology Kidney Tubules - pathology Kidneys Male Medical sciences Mice Mice, Inbred C57BL Nephrology. Urinary tract diseases podocyte Postoperative Period Regeneration tubular cells Urinary system involvement in other diseases. Miscellaneous Y Chromosome Human Immunohistochemistry Kidney disease Urinary system disease Stem cell In situ Rodentia Parenchyma Hybridization Pathology Regeneration Vertebrata Mammalia Mouse Animal Turnover Bone marrow Molecular biology
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Summary:	In order to establish whether extra‐renal cells contribute to the turnover and repair of renal tissues, this study examined kidneys of female mice that had received a male bone marrow transplant and kidney biopsies from male patients who had received kidney transplants from female donors. By using in situ hybridization to detect Y‐chromosomes it could be demonstrated that circulating stem cells frequently engraft into the kidney and differentiate into renal parenchymal cells. In the human renal grafts it was confirmed that some of the recipient‐derived cells within the kidney exhibited a tubular epithelial phenotype, by combining in situ hybridization with immunostaining for the epithelial markers CAM 5.2 and the lectin Ulex europaeus. Female mouse recipients of male bone marrow grafts showed co‐localization of Y‐chromosomes and tubular epithelial markers Ricinus communis and Lens culinaris, and a specific cytochrome P450 enzyme (CYP1A2) indicating an appropriate functional capability of clustered newly formed marrow‐derived tubular epithelial cells. Y‐chromosome‐containing cells were observed within glomeruli, with morphology and location appropriate for podocytes. Within the murine kidney, these Y‐chromosome‐positive cells were negative for the mouse macrophage marker F4/80 antigen and leukocyte common antigen, but were vimentin‐positive. The presence of bone marrow‐derived cells was noted in both histologically normal mouse kidneys and in human transplanted kidneys suffering damage from a variety of causes. These data indicate that bone marrow cells contribute to both normal turnover of renal epithelia and regeneration after damage, and it is suggested that this could be exploited therapeutically. Copyright © 2001 John Wiley & Sons, Ltd.
Bibliography:	ark:/67375/WNG-BPF41WMJ-Z istex:825DC389ECBFD557B1B4D06CCCDFBCBBCAC70358 ArticleID:PATH976 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-3417 1096-9896
DOI:	10.1002/path.976