Physical Mapping of 18S rRNA Gene in Green Mussel Perna viridis – An Indication of Higher Major rRNA Gene Clusters

Physical Mapping of 18S rRNA Gene in Green Mussel Perna viridis – An Indication of Higher Major rRNA Gene Clusters

The family Mytilidae generally shows a genus-specific diploid number (2 n ) of chromosomes; however, one species from each genus, with the exception of Mytilus , is known to have a variable diploid number. Perna viridis is a cytogenetically significant species because it has a diploid chromosome (2...

Full description

Saved in:
Bibliographic Details
Published in:Russian journal of marine biology Vol. 48; no. 3; pp. 195 - 201
Main Authors: Muhammed Zafar Iqbal, A. N., Khan, Mohd Sajid, Navalgund, M. A., Goswami, U.
Format: Journal Article
Language:English
Published: Moscow Pleiades Publishing 01-06-2022
Springer Nature B.V
Subjects:
Biomedical and Life Sciences
Chromosomes
Cytogenetics
Diploids
Diploidy
Fluorescence
Freshwater & Marine Ecology
Gene clusters
Gene mapping
Life Sciences
Marine molluscs
Metaphase
Mytilus
Nucleotide sequence
Original Papers
Perna viridis
Physical mapping
rRNA 18S
Silver
Species
phylogenetics
physical mapping
Mytilidae
flourescence in situ hybridization
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The family Mytilidae generally shows a genus-specific diploid number (2 n ) of chromosomes; however, one species from each genus, with the exception of Mytilus , is known to have a variable diploid number. Perna viridis is a cytogenetically significant species because it has a diploid chromosome (2 n ) number of 30, whereas the rest of species of genera Perna and Mytilus have a diploid number of 28. In the present study, we amplified the partial sequence of the 18S rRNA gene of Perna viridis , and the fluorescence (DIG-dUTP) labeled probe was hybridized to the interphase and metaphase chromosomes. The results localize the 18S rRNA gene clusters on the short arms of four chromosome pairs 1, 3, 7, and 11. This coincides with the position of the NORs by silver staining. The findings of the present study indicate a greater number of major rDNA clusters in Perna viridis than in closely related species. This is the first comprehensive molecular cytogenetic study carried out in Perna viridis.
ISSN:1063-0740
1608-3377
DOI:10.1134/S1063074022030038