The anal pore route is efficient to infect Amblyomma spp. ticks with Rickettsia rickettsii and allows the assessment of the role played by infection control targets
Adult Amblyomma sculptum and Amblyomma aureolatum ticks are partially refractory to Rickettsia rickettsii when fed on infected hosts, hindering the functional characterization of potentially protective targets in the bacterial acquisition. In the current study, we used the anal pore route to infect...
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| Published in: | Frontiers in cellular and infection microbiology Vol. 13; p. 1260390 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Frontiers Media S.A
12-10-2023
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Adult
Amblyomma sculptum
and
Amblyomma aureolatum
ticks are partially refractory to
Rickettsia rickettsii
when fed on infected hosts, hindering the functional characterization of potentially protective targets in the bacterial acquisition. In the current study, we used the anal pore route to infect adult
A. sculptum
and
A. aureolatum
ticks with
R. rickettsii
and to assess the effects of the knockdown of microplusin in infection control. The anal pore route was efficient to infect both species, resulting in a prevalence of around 100% of infected ticks. Higher loads of
R. rickettsii
were detected in microplusin-silenced
A. aureolatum
in relation to the control, as previously obtained when microplusin-silenced ticks were fed on
R. rickettsii
-infected rabbits. This is the first report showing
R
.
rickettsii
infection through the anal pore in
Amblyomma
ticks, highlighting this route as a powerful tool to assess the role played by additional targets in the control of pathogens. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Benjamin Cull, University of Minnesota Twin Cities, United States; Girish Neelakanta, University of Tennessee, United States Edited by: Isaura Simões, University of Coimbra, Portugal These authors have contributed equally to this work These authors have contributed equally to this work and share senior authorship Present addresses: Eliane Esteves, Department of Microbiology and Immunology, College of Medicine, University of South Alabama, Mobile, AL, United States; Daniel B. Pavanelo, Laboratório Central do Rio Grande do Sul, Centro Estadual de Vigilância em Saúde, Secretaria da Saúde, Porto Alegre, Brazil |
| ISSN: | 2235-2988 2235-2988 |
| DOI: | 10.3389/fcimb.2023.1260390 |