Liver microsomal membrane fluidity and microsomal desaturase activities in adult spontaneously hypertensive rats

OBJECTIVEThe purpose of the present study was to investigate liver microsomal membrane fluidity simultaneously with membrane fatty acid composition and desaturase activities in spontaneously hypertensive rats (SHR). DESIGN AND METHODSThe membrane fluidity was determined, after electron spin resonanc...

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Published in:	Journal of hypertension Vol. 15; no. 8; pp. 863 - 869
Main Authors:	Foucher, Christelle, Narce, Michel, Nasr, Laure, Delachambre, Marie-Claire, Poisson, Jean-Pierre
Format:	Journal Article
Language:	English
Published:	Hagerstown, MD Lippincott-Raven Publishers 01-08-1997 Lippincott Williams & Wilkins
Subjects:	Animals Arterial hypertension. Arterial hypotension Biological and medical sciences Blood and lymphatic vessels Cardiology. Vascular system Electron Spin Resonance Spectroscopy Experimental diseases Fatty Acid Desaturases - metabolism Fatty Acids, Unsaturated - metabolism Female Intracellular Membranes - metabolism Male Medical sciences Membrane Fluidity Microsomes, Liver - enzymology Microsomes, Liver - metabolism Rats Rats, Inbred SHR Rats, Inbred WKY Hypertension Composition Pathophysiology Rat Digestive system Liver Rodentia Cardiovascular disease Lipids Hereditary Microsome Fatty acids Vertebrata Mammalia Enzymatic activity Fluidity Animal Membrane
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Summary:	OBJECTIVEThe purpose of the present study was to investigate liver microsomal membrane fluidity simultaneously with membrane fatty acid composition and desaturase activities in spontaneously hypertensive rats (SHR). DESIGN AND METHODSThe membrane fluidity was determined, after electron spin resonance (ESR) measurement, in SHR compared with normotensive Wistar–Kyoto (WKY) rats, by calculating the order parameter S from ESR spectra of 5-nitroxide stearate and 10-nitroxide stearate, used as spin-labelled fatty acids. Desaturase activities were measured by incubating SHR and WKY rat liver microsomes with [14C]-radiolabeled fatty acids as substrates for desaturation reactions. The fatty acid composition of liver microsomal membranes was determined by gas–liquid chromatography. RESULTSWhereas no significant difference between S of 5-nitroxide stearate was observed for SHR and WKY rats, S of 10-nitroxide stearate was significantly lower in SHR than it was in WKY rat microsomal membrane, indicating that the core microsomal membrane fluidity was higher in SHR. Significant differences between fatty acid compositions were observed for SHR and WKY rat microsomal membranes. Δ9 and n-6 Δ6 microsomal desaturase activities were significantly lower in SHR. CONCLUSIONThese results suggest that the higher liver core microsomal membrane fluidity observed in SHR might be dependent on the increased proportion of mono-unsaturated fatty acids. Such observed modifications and the alterations in Δ9 and n-6 Δ6 desaturase activities suggest that an impaired polyunsaturated fatty acid biosynthesis is related to changes in microsomal membrane fluidity in hypertension.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0263-6352 1473-5598
DOI:	10.1097/00004872-199715080-00010