Cultured allogeneic fibroblast injection vs. fibroblasts cultured on amniotic membrane scaffold for dystrophic epidermolysis bullosa treatment
Summary Background Different methods of fibroblast application have been examined to treat recessive dystrophic epidermolysis bullosa (RDEB). Objectives To compare the effects of intradermal injection of cultured allogeneic fibroblasts in healing RDEB wounds with those of fibroblasts seeded on amnio...
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| Published in: | British journal of dermatology (1951) Vol. 179; no. 1; pp. 72 - 79 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
England
01-07-2018
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| Online Access: | Get full text |
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| Summary: | Summary
Background
Different methods of fibroblast application have been examined to treat recessive dystrophic epidermolysis bullosa (RDEB).
Objectives
To compare the effects of intradermal injection of cultured allogeneic fibroblasts in healing RDEB wounds with those of fibroblasts seeded on amniotic membrane scaffolds (FAMS) or standard wound care (SWC) with Vaseline® gauze as controls.
Methods
Seven patients were recruited, and seven wounds were assessed in each patient: three wounds were treated with injection of intradermal fibroblasts, three were treated with FAMS and one was dressed with SWC. Changes in wound size were assessed after 2 and 12 weeks of treatment. Qualitative wound scores (QWS) were used to assess wound severity. Additionally, biopsies and antigen mapping were performed to detect type VII collagen in the dermoepidermal junction.
Results
In both treated areas, the QWS and wound size were significantly decreased (P < 0·001), whereas there were no changes in the control group (P = 0·29). After 2 and 12 weeks of treatment, the wound size was significantly decreased in wounds that were treated with fibroblast injection compared with those treated with FAMS (P < 0·001); but no significant changes were found in the control group.
Conclusions
Fibroblast injection has been shown to promote healing of RDEB wounds and is superior to FAMS or the control treatment.
What's already known about this topic?
Currently, there is no definitive treatment for recessive dystrophic epidermolysis bullosa (RDEB).
Cell‐based therapy is a promising approach to treat the wounds of patients with RDEB.
Amniotic membrane has been used in dressing and promoting closure of epidermolysis bullosa wounds.
What does this study add?
In this study, the effects of intradermal injection of cultured allogeneic fibroblasts in healing RDEB wounds were compared with those of fibroblasts seeded on amniotic membrane scaffolds (FAMS).
In treated areas (using intradermal fibroblast injection or FAMS) the qualitative wound scores and wound size were significantly decreased.
After 2 and 12 weeks of treatment, wound size was significantly decreased in wounds that were treated with fibroblast injection compared with those treated with FAMS.
Plain language summary available online |
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| Bibliography: | Plain language summary available online ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
| ISSN: | 0007-0963 1365-2133 |
| DOI: | 10.1111/bjd.16338 |